Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer

Identify, quantify and confirm more compounds rapidly and with confidence using the Thermo Scientific™ Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer. This benchtop LC-MS/MS system combines quadruple precursor ion selection with high-resolution, accurate-mass (HRAM) Orbitrap detection to deliver exceptional performance and versatility. The Q Exactive Mass Spectrometer is equally useful for untargeted or targeted screening and a broad range of qualitative and quantitative applications in drug discovery, proteomics, environmental and food safety, clinical research and forensic toxicology.

Genotoxic impurity analysis in pharmaceutical products

The Food and Drug Administration (FDA) released a liquid chromatography-high resolution mass spectrometry (LC-HRMS) method for the quantitation of N-nitrosodimethylamine (NDMA) in Ranitidine drug substance and drug products using the Q Exactive hybrid quadrupole-Orbitrap mass spectrometer.

High-Confidence Results, Quickly

• HRAM and full-scan capabilities capture all sample data, all the time, enabling retrospective data analysis without need to re-run samples
• Resolving power up to 140,000 FWHM eliminates isobaric interferences, increasing confidence in results when analyzing samples in complex matrices
• Better than 1 ppm mass accuracy in full-scan and all-ion fragmentation (AIF) modes ensures confident compound identification
• Fast scanning at 12 hz and spectral multiplexing suited to UHPLC applications
• Rapid polarity switching maximizes information obtained
• AIF and multiple dissociation techniques—in-source CID and HCD—aid in compound identification
• Extended mass range to 6000 m/z enhances detection of singly charged small molecules and biomolecules
• More than four orders of magnitude intrascan dynamic range, along with femtogram-level sensitivity, allow detection of trace-level and high-abundance compounds in the same scan

Greater Confidence for Drug Discovery and Metabolism Studies

• Triple quadruple-equivalent ability to quantify parent compounds and metabolites along with identity confirmation provides greater throughput
• Fast HRAM Orbitrap technology eliminates interferences for high-confidence metabolite structural identification and large-scale profiling of metabolomics and lipidomics samples
• Fast scan-to-scan polarity switching in both MS and MS/MS modes maximizes metabolite detection and identification from a single injection

More Information for Superior Proteomics

• HCD yields superior fragmentation and higher-quality MS/MS spectra compared to CID, improving identifications
• High spectral quality and a wide dynamic range make it possible to identify and quantify proteins other instruments cannot find.
• Quan/qual capability make it easier to transition from proteomic discovery experiments to targeted protein quantification

Highly Specific Environmental and Food Safety Analyses

• Specificity exceeding triple quadruple Q-TOF or Q-Trap levels make this mass spectrometer the preferred quan/qual screening platform
• Data-dependent, full-scan MS/MS capabilities and HRAM data ensure the highest possible confidence in every identification

High-Confidence Quanfirmation for Clinical Research and Forensic Toxicology

• The combination of SRM and HRAM capabilities provides high-confidence quanfirmation of endogenous steroids, drugs of abuse and pain-management therapeutics with ease.

Application-Specific Software for Greater Throughput

• Thermo Scientific™ ExactFinder™, LCQuan™, Mass Frontier™, MetQuest™, MetWorks™, SIEVE™ and TraceFinder™ software are available to speed data analysis.