PureLink™ Genomic DNA Mini Kit
PureLink™ Genomic DNA Mini Kit
Invitrogen™

PureLink™ Genomic DNA Mini Kit

The PureLink™ Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types.Read more
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Catalog NumberQuantity
K182002250 Preps
K18200010 Preps
K18200150 Preps
Catalog number K182002
Price (CNY)/ Each
Price:7,147.00
Online offer:5,632.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
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Quantity:
250 Preps
Price (CNY)/ Each
Online offer:5,632.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
Add to cart
PureLink™ Genomic DNA Mini Kit
Catalog numberK182002
Price (CNY)/ Each
Online offer:5,632.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
-
Add to cart

The PureLink™ Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format. Features of the PureLink™ Genomic DNA Mini Kit include:

Kit flexibility—one kit works with a variety of sample types and sizes
Ultraclean gDNA—minimal contamination of DNA product means successful downstream application
Improved design—optimized spin-column design and buffer formulation for better yield and purity

Multiple sample sources, one kit
The PureLink™ Genomic DNA Mini Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual.

Optimized components and protocols
The PureLink™ Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use (see figure). Columns are newly configured to bind and release DNA more efficiently (see figure). Each sample source uses a specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination. A chaotropic salt buffer promotes stable binding of DNA to the column resin, and powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.

Higher yields and purity
With PureLink™ Genomic DNA Kits you can expect high yields of high-purity gDNA (determined by A260/A280 measurements), no matter the sample type, from bacteria to tissues to blood to cultured cells (see figures). The enhanced PureLink™ Lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ∼1.9 for both A260/A280 and A260/A230, indicating no detectable contamination from carryover of protein or guanidine (see figure).

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Isolation TechnologySilica Spin Column
ScaleMini
Sample TypeBacteria, Cells, Dried Blood Spots, FFPE (Formalin-fixed paraffin-embedded), Mouse/Rat Tail, Saliva, Buccal Swab, Tissue, Whole Blood, Yeast
Elution Volume25 to 200 μL
Final Product TypegDNA
For Use With (Application)PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Quantity250 Preps
Shipping ConditionRoom Temperature
Test TimeUp to 15 min. after cell lysis
Yield≤40 μg
Starting Material AmountBacteria: ≤109 cells
Cells: ≤106
Tissue: ≤25 mg
Mouse Tail: 1 cm
Rat Tail: 0.5 cm
Whole Blood: ≤1 mL
Yeast: ≤107 cells
FFPE: ≤8 sections of 5-15 μM thickness
Oragene™ Preserved Saliva: ≤4 mL
Blood Spots: ≤5 punches
Unit SizeEach
Contents & Storage
• 50 mL PureLink Genomic Lysis/Binding Buffer; room temperature
• 45 mL PureLink Genomic Digestion Buffer; room temperature
• 50 mL PureLinkGenomic Wash Buffer 1; room temperature
• 37.5 mL PureLink Genomic Wash Buffer 2; room temperature
• 50 mL PureLink Genomic Elution Buffer; room temperature
• 5 mL RNase A (20 mg/mL); room temperature
• 5 mL Proteinase K (20 mg/mL); room temperature, or 4°C for long-term storage
• 5 x 50 PureLink Spin Columns with Collection Tubes; room temperature
• 5 x 100 PureLink Collection Tubes; room temperature