Contents & Storage
Each pcDNA™6.2-TOPO™ Expression Vector Kit contains two boxes. The TOPO™ box contains linearized and topoisomerase I-activated pcDNA™6.2-TOPO™ vector, control template, primers for sequencing, PCR reaction components, and an expression control plasmid. The TOPO™ box should be stored at -20°C. The One Shot™ box contains transformation reagents including single-use 50 μl aliquots of One Shot™ TOP10 chemically competent E. coli, S.O.C. medium, and pUC19 supercoiled plasmid control. Store competent E. coli at -80°C. All reagents are guaranteed stable for 6 months when properly stored.