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Invitrogen™
pcDNA™3.1 Directional TOPO™ Expression Kit
The pcDNA™3.1 Directional TOPO™ Expression Kit uses linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO™ for five-minute directional cloning and subsequent high-level expression.Directional Cloning Read more
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| Catalog Number | Quantity |
|---|---|
| K490001 | 20 Reactions |
| K490040 | 40 Reactions |
Catalog number K490001
Price (CNY)/ 20 reactions
Price:14,574.00
Online offer:12,388.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
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Quantity:
20 Reactions
Price (CNY)/ 20 reactions
Online offer:12,388.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
pcDNA™3.1 Directional TOPO™ Expression Kit
Catalog numberK490001
Price (CNY)/ 20 reactions
Online offer:12,388.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
-
The pcDNA™3.1 Directional TOPO™ Expression Kit uses linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO™ for five-minute directional cloning and subsequent high-level expression.
Directional Cloning technology facilitates expression experiments because:
• A proofreading enzyme is used for fewer errors in cloned genes
• Greater than 90% of the clones are in the correct orientation for expression, reducing time spent colony screening for clone orientation
In addition, pcDNA3.1D/V5-His-TOPO™ provides strong expression levels from the CMV promoter and the option of a C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody and rapid purification on nickel-chelating resin.
Directional Cloning technology facilitates expression experiments because:
• A proofreading enzyme is used for fewer errors in cloned genes
• Greater than 90% of the clones are in the correct orientation for expression, reducing time spent colony screening for clone orientation
In addition, pcDNA3.1D/V5-His-TOPO™ provides strong expression levels from the CMV promoter and the option of a C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody and rapid purification on nickel-chelating resin.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible SystemConstitutive
Delivery TypeTransfection
PromoterCMV
Product TypeTOPO Expression Kit
Selection Agent (Eukaryotic)Geneticin™ (G-418)
Protein TagHis Tag (6x), V5 Epitope Tag
Cloning MethodDirectional TOPO
Quantity20 Reactions
VectorDirectional TOPO Vectors, pcDNA
Product LineTOPO™, pcDNA™
For Use With (Application)Constitutive Expression
Unit Size20 reactions
Contents & Storage
Box 1 (Store at -20°C):
• 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl)
• 10 μl dNTP Mix
• 50 μl Salt Solution
• 1 ml Sterile Water
• 20 μl T7 Sequencing Primer (0.1 μg/μl)
• 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl)
• 10 μl Control PCR Template (0.1 μg/μl)
• 10 μl Control PCR Primers (0.1 μg/μl each)
• 10 μl Expression Plasmid (0.5 μg/μl)
Box 2 (Store at -80°C):
• 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)
• 21 × 50 μl TOP10 Cells
• 50 μl pUC19 Control DNA
• 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl)
• 10 μl dNTP Mix
• 50 μl Salt Solution
• 1 ml Sterile Water
• 20 μl T7 Sequencing Primer (0.1 μg/μl)
• 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl)
• 10 μl Control PCR Template (0.1 μg/μl)
• 10 μl Control PCR Primers (0.1 μg/μl each)
• 10 μl Expression Plasmid (0.5 μg/μl)
Box 2 (Store at -80°C):
• 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)
• 21 × 50 μl TOP10 Cells
• 50 μl pUC19 Control DNA