Expressway™ Mini Cell-Free Expression System

The Expressway™ Mini Cell-Free Expression System utilizes an efficient coupled transcription and translation reaction to produce active recombinant protein. This system eliminates the time-consuming steps of cell-based protein production including transformation, cell culture, and expression optimization. In just two hours, you can produce protein suitable for an array of downstream applications and functional studies. The Expressway™ Mini Cell-Free Expression System offers flexibility in experimental design, allowing you to
• Synthesize proteins from both circular plasmid and linear templates (e.g. PCR products)
• Perform high-throughput synthesis of proteins
• Express genes toxic to in vivo systems

How it works:
The Expressway™ Mini Cell-Free Expression System provides all the components you need for optimal cell-free protein production. The kit includes an E. coli extract containing the cellular machinery required to drive transcription and translation. An IVPS (in vitro protein synthesis) reaction buffer and an amino acid module are also included in the kit to provide the required amino acids and an ATP-regenerating system. Simply mix the reaction buffer, amino acid mix, methionine (labeled or unlabeled), T7 Enzyme Mix, and your DNA template (containing a T7 promoter) with the E. coli extract. As the DNA template is transcribed, the 5’ end of the mRNA is bound by ribosomes and undergoes translation as the 3’ end of the template is still being transcribed (Figure 1).


Functional protein made easy:

The Expressway™ Mini Cell-Free Expression System has been optimized to produce high yields of full-length, functional protein from E. coli cell lysates (Figure 2). A proprietary feed buffer formulation is provided with the system for increased protein yields.


Contents and Storage:
The Expressway™ Mini Cell-Free Expression System is designed to perform twenty 50-μl reactions or one 1-ml reaction. This system includes the IVPS E.coli Extract, IVPS reaction buffer, feed buffer, 19 amino acid mix, methionine, DNase/RNase-free water, RNase A, T7 Enzyme Mix, 2-ml reaction tubes, and a positive expression control vector. Suggested vectors for use with the Expressway™ Mini Cell-Free Expression System are listed below. Store vectors at -20 °C. Store other kit components at -80 °C.