iBright™ Prestained Protein Ladder
iBright™ Prestained Protein Ladder
Invitrogen™

iBright™ Prestained Protein Ladder

iBright Prestained Protein Ladder contains twelve recombinant proteins, ten (11 to 250 kDa) that are blue-stained and fluor-labeled for directRead more
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Catalog number LC5615
Price (CNY)/ Each
Price:1,995.00
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Quantity:
2 x 250 μL
Price (CNY)/ Each
Price:1,995.00
Add to cart
iBright™ Prestained Protein Ladder
Catalog numberLC5615
Price (CNY)/ Each
Price:1,995.00
-
Add to cart
iBright Prestained Protein Ladder contains twelve recombinant proteins, ten (11 to 250 kDa) that are blue-stained and fluor-labeled for direct and near-IR fluorescent visualization and protein sizing, and two (30 kDa and 80 kDa) that are unstained and contain IgG binding sites to bind the primary and secondary antibodies used for chemiluminescent and fluorescent detection of the target protein. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Product features
• Fluorescent visualization—detect the 10 stained proteins using the 670 nm red laser or 700 nm channel of a fluorescence imager
• Western blot confirmation—detect the two unstained proteins (30 kDa and 80 kDa) using the detection system for the target protein

Applications
• Western blotting: detection of the two unstained bands via the detection method used for the target protein. Compatible with chemiluminescent substrates and fluorescent secondary antibodies.
• Protein sizing on SDS-polyacrylamide gels, visualization of proteins during electrophoresis and transfer, and visualization of proteins during fluorescence imaging (ten blue-stained bands)
• Protein size estimation: protein sizing on SDS-polyacrylamide gels and on western blot using near-infrared (NIR) fluorescence imagers

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodColorimetric, NIR Fluorescence, and User defined detection system (2 bands)
Number of Markers12
Product TypeProtein Ladder
Ready to LoadYes
Size Range11 to 250 kDa
Stain Type1 color: Blue
Gel CompatibilityNovex™ Midi Gels, Novex™ Mini Gels, Novex™Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Gels, NuPAGE™ MES Gels, NuPAGE™ MOPS Gels, NuPAGE™ Tris-Acetate Gels, Precise™ Tris-Glycine Gels, SDS-PAGE Gels, Precise™ Tris-HEPES Gels
Molecular Weight (g/mol)250, 130, 95, 80, 70, 55, 43, 34, 30, 26, 15, 11 kDa
Product LineiBright™
Quantity2 x 250 μL
Shipping ConditionDry Ice
System TypeWestern Blotting, SDS-PAGE
Unit SizeEach
Contents & Storage
Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% (w/v) SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol

Upon receipt, store at –20°C. Product is shipped on ice.