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Catalog Number | Unit Size | Price (CNY) | Availability | Quantity | |
---|---|---|---|---|---|
M1632 | 100 reactions | Price: 1,262.00 Online offer: 631.00 (ends 31-Dec-2024) Your price: | - | ||
M1631 | 50 reactions | Price: 710.00 Online offer: 355.00 (ends 31-Dec-2024) Your price: | - |
Thermo Scientific RevertAid Master Mix is a complete system for efficient synthesis of first-strand cDNA from mRNA or total RNA templates. The mix includes RevertAid Reverse Transcriptase (RT), which has lower RNase H activity compared to AMV reverse transcriptase. RevertAid Master Mix provides all cDNA synthesis reaction components in a convenient two-tube master mix. It is optimized for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications. The two-tube formulation has a shorter reaction time and requires fewer pipetting steps than the RevertAid RT Reverse Transcription Kit, while maintaining high performance.
Highlights
• Fast—short reaction time (typically 15–30 min)
• Convenient—easy workflow with few pipetting steps
• Reliable—reverse transcription of both abundant and limited targets
• Flexible—optimized 2-step protocol, enabling multiple PCR reactions from a single reverse-transcription reaction
Applications
First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis
• 20X Enzyme mix (2 x 50 μL)
• 2X RT Buffer Mix (2 x 500 μL)
Store at –15 to –25°C.
2X RT Buffer Mix can be stored at 2–8°C for up to 6 months.