The MitoProbe™ DiOC2(3) Assay Kit provides solutions of the membrane-potential-sensitive cyanine dye DiOC2(3), and a mitochondrial membrane-potential disrupter, CCCP, forRead more
The MitoProbe™ DiOC2(3) Assay Kit provides solutions of the membrane-potential-sensitive cyanine dye DiOC2(3), and a mitochondrial membrane-potential disrupter, CCCP, for the study of mitochondrial membrane potential. DiOC2(3) penetrates the cytosol of eukaryotic cells and accumulates primarily in mitochondria with active membrane potentials, producing bright, red fluorescence. DiOC2(3) staining intensity decreases with decreased mitochondrial membrane potential, accompanied by a shift from red to green fluorescence.