NeuroTrace™ BDA-10,000 Neuronal Tracer Kit

Citations & References (6)

Invitrogen™

NeuroTrace™ BDA-10,000 Neuronal Tracer Kit

The NeuroTrace™ BDA-10,000 Neuronal Tracer Kit contains the neuronal tracer, biotin dextran amine (BDA), and the detection reagents, avidin-horseradish peroxidaseRead more

Catalog Number	Quantity
N7167	1 kit

Catalog number N7167

Price (CNY)

6,895.00

Add to cart

Quantity:

1 kit

Price (CNY)

6,895.00

Add to cart

The NeuroTrace™ BDA-10,000 Neuronal Tracer Kit contains the neuronal tracer, biotin dextran amine (BDA), and the detection reagents, avidin-horseradish peroxidase (avidin-HRP) and diaminobenzedine (DAB) for subsequent visualization by light or electron microscopy.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection MethodColorimetric

For Use With (Equipment)Electron Microscope, Light Microscope

Product TypeNeuronal Tracer Kit

Quantity1 kit

Shipping ConditionWet Ice

Sub Cellular LocalizationCytoplasm & Cytosol

Product LineNEUROTRACE

Unit SizeEach

Contents & Storage

Store in freezer (-5 to -30°C) and protect from light.

Frequently asked questions (FAQs)

I can't see the structural details of neurons when I inject my fluorescent dextran. What can I do to improve the detailed structure?

If you want to see the most detailed structure you should use the low molecular weight conjugated dextrans such as the 3,000 MW dextrans.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why isn't my fluorescently conjugated dextran signal retained after fixation?

Ensure that the dextran you are using is the fixable form (i.e., contains a primary amine). Dextrans that do not contain a primary amine will not be fixed. Another factor could be that the concentration of the dextran is too low, and the concentration use can be increased up to 10 mg/mL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the charges of the dextrans?

We do not determine the net charge of the dextran conjugates. The net charge depends on the fluorophore used to label the dextran and the method of preparing the conjugate. We label some dextrans as neutral or anionic based on the fluorophore used, however the net charge of the dextran may not always be the same as the dye. The Alexa Fluor, Cascade Blue, Lucifer Yellow, fluorescein, and Oregon Green dextrans are intrinsically anionic, whereas most of the dextrans labeled with the zwitterionic Rhodamine B, tetramethylrhodamine and Texas Red dyes are essentially neutral.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What size dextran is best for neuronal tracing?

Dextrans with molecular weights from 3,000 to 70,000 have been used, however the 3,000 and 10,000 MW dextrans are most commonly used for neuronal tracing. The 3,000 MW dextrans are used for more detailed tracing of fine neuronal projections, investigating gap junctions, and diffuse more quickly; while the 10,000 MW dextrans have slower distribution, longer cellular retention, and do not cross gap junctions.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you have a neuronal tracing protocol?

The NeuroTrace BDA-10,000 Neuronal Tracer Kit (Cat. No. N7167) manual has a good protocol for injection procedures and neuronal tracing using the10,000 MW lysine-fixable biotin dextran amine (BDA). This protocol could potentially be applied to other fluorescent dextrans.

Please review Tables 1a and 1b on pages 4 and 5 - https://tools.thermofisher.com/content/sfs/manuals/mp07167.pdf

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all NeuroTrace™ BDA-10,000 Neuronal Tracer Kit FAQs

Citations & References (6)

Citations & References

Abstract

Complex brain circuits studied via simultaneous and permanent detection of three transported neuroanatomical tracers in the same histological section.

Authors:Lanciego JL, Wouterlood FG, Erro E, Arribas J, Gonzalo N, Urra X, Cervantes S, Giménez-Amaya JM

Journal:J Neurosci Methods

PubMed ID:11074102

'Experimental neuroanatomical tracing methods lie at the basis of the study of the nervous system. When the scientific question is relatively straightforward, it may be sufficient to derive satisfactory answers from experiments in which a single neuroanatomical tracing method is applied. In various scientific paradigms however, for instance when the ... More

Giant neurons in the macaque pulvinar: a distinct relay subpopulation.

Authors:Imura K, Rockland KS,

Journal:Front Neuroanat

PubMed ID:18958196

Calbindin positive (CB+) giant neurons are known to occur within the pulvinar nucleus in subhuman primates. Here, we demonstrate by combined retrograde tracing and immunocytochemistry that at least some of these are pulvinocortical relay neurons, and further report several distinctive features. First, in contrast with non-giant relay neurons, the giant ... More

Selective anterograde tracing of the individual serotonergic and nonserotonergic components of the dorsal raphe nucleus projection to the vestibular nuclei.

Authors:Halberstadt AL, Balaban CD

Journal:Neuroscience

PubMed ID:17507165

It is well known that the dorsal raphe nucleus (DRN) sends serotonergic and nonserotonergic projections to target regions in the brain stem and forebrain, including the vestibular nuclei. Although retrograde tracing studies have reported consistently that there are differences in the relative innervation of different target regions by serotonergic and ... More

Novel applications of common stereology software to represent the complete distribution, density and spatial organization of anterogradely labelled fibers in neuroanatomical tract-tracing studies.

Authors:Leite-Almeida H, Almeida A

Journal:J Neurosci Methods

PubMed ID:17368789

In a recent study we have adapted for the first time available software used in spatial neuronal organization studies to represent the complete fiber distribution of a brain projection [Leite-Almeida H, Valle-Fernandes A, Almeida A. Brain projections from the medullary dorsal reticular nucleus: an anterograde and retrograde tracing study in ... More

ALK5 signaling pathway mediates neurogenesis and functional recovery after cerebral ischemia/reperfusion in rats via Gadd45b.

Authors:

Journal:Cell Death Dis

PubMed ID:31043581

View all NeuroTrace™ BDA-10,000 Neuronal Tracer Kit citations