pHrodo™ Green Maleimide
pHrodo™ Green Maleimide
Invitrogen™

pHrodo™ Green Maleimide

The thiol-reactive pH-sensitive pHrodo™ Green Maleimide dye is suitable for the creation of bioconjugates to study endocytosis and phagocytosis. pHrodo™Read more
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Catalog number P35370
Price (CNY)/ Each
Price:9,677.00
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Quantity:
1 mg
Price (CNY)/ Each
Price:9,677.00
Add to cart
pHrodo™ Green Maleimide
Catalog numberP35370
Price (CNY)/ Each
Price:9,677.00
-
Add to cart
The thiol-reactive pH-sensitive pHrodo™ Green Maleimide dye is suitable for the creation of bioconjugates to study endocytosis and phagocytosis. pHrodo™ Green dramatically increases fluorescence as the pH of its surroundings become more acidic.
• Use pH-sensitive pHrodo™ Green Maleimide to make pH-sensitive bioconjugates of your choice
• Get faster, more accurate results than with any other endocytosis or phagocytosis assay—no need for wash steps or quenchers
• Multiplex with red fluorescent markers such as RFP, pHrodo™ Red, and many others

The increase in fluorescence of pHrodo™ Green as pH changes from basic to acidic correlates with the acidification of intracellular vesicles, making it an ideal tool to study endocytosis or phagocytosis and their regulation by environmental factors, drugs, or pathogens. The spectral properties of pHrodo™ Green makes it useful for multi-color experiments. pHrodo™ Green can be detected using most standard green fluorescent filter sets and has been validated for use on a variety of platforms including flow cytometry, fluorescent microscopy, and high content screening (HCS). The lack of fluorescence of pHrodo™ Green in a typical extracellular environment eliminates the need for wash steps or quencher dyes in the experimental workflow.

pHrodo™ Green Maleimide is a thiol-reactive dye that can be used to create pHrodo™ Green bioconjugates in aqueous buffer. The maleimide reacts with free sulphydryl groups produced by the reduction of cysteines in proteins or peptides. Maleimides are particularly useful for labeling antibodies as the dye will not attach to the antibody binding site. This reaction will result in a stable conjugate that can be used in live cell assays or stored for later use.

pHrodo™ Green is also available in an amine-reactive form (see pHrodo™ Green STP), as well as a selection of ready-to-use conjugates (e.g., E. coli, S. aureus, and dextran). In addition, pHrodo™ Red reactive dyes and ready-to-use conjugates are available as a color alternative with the same properties.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatDried Down
Quantity1 mg
Product TypeMaleimide
SolubilityDMSO (Dimethylsulfoxide)
Chemical ReactivityThiol
Detection MethodFluorescence
EC Extinction Coefficient75000
Reactive GroupThiol
Label or DyepHrodo™ Green
ColorGreen
Product LinepHrodo™
Emission525
Excitation505
Unit SizeEach
Contents & Storage
1 vial , store ≤ -20 °C, dessicate and protected from light.