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Z'-LYTE™ Kinase Assay Kit - Ser/Thr 1 Peptide
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is Read more
Catalog number PV3174
Price (CNY)/ 800 x 20 µL assays
Price:11,857.00
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Price (CNY)/ 800 x 20 µL assays
Price:11,857.00
Z'-LYTE™ Kinase Assay Kit - Ser/Thr 1 Peptide
Catalog numberPV3174
Price (CNY)/ 800 x 20 µL assays
Price:11,857.00
-
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon a porcine pyruvate liver kinase derived peptide used to study PKA substrate specificity, and containing an R-R-X-S consensus sequence, where X is any residue.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product LineZ´-LYTE™
TargetSerine/Threonine Kinases
For Use With (Application)FRET
AssaySerine/Threonine Kinase Assay
Assay EntryBiochemical phospho-specific activity (no antibody)
Label or DyeMethylcoumarin
Product TypeKinase Assay Kit
Quantity800 x 20 μL Assays
Format384-well plate
Sample Typeporcine pyruvate liver kinase (LRRASLG)
Excitation/Emission405⁄465, 535
No. of Reactions800 Reactions
ReferencesBBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978)
ReadoutEnd Point
Shipping ConditionDry Ice
SubstratePeptide-Based Substrate
Target EntryPRKX, PRKG2, RPS6KA5, PRKACA, PRKG1, RPS6KA4, AURKB, AURKA
Validated KinasesAURKA, AURKB, PRKACA, PRKG1, PRKG2, PRKX, RPS6KA4, RPS6KA5
Unit Size800 x 20 µL assays
Contents & Storage
Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.