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Z'-LYTE™ Kinase Assay Kit - Ser/Thr 4 Peptide
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide isRead more
Catalog number PV3177
Price (CNY)/ Each
Price:11,881.00
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Price (CNY)/ Each
Price:11,881.00
Z'-LYTE™ Kinase Assay Kit - Ser/Thr 4 Peptide
Catalog numberPV3177
Price (CNY)/ Each
Price:11,881.00
-
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon glycogen synthase, containing Ser-7 and a consensus seq Hyd-X-R-X-X-S/T-X-X, where Hyd is bulky hydrophobic, F > L > V >> A, and X is any residue.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product LineZ ́-LYTE™
AssaySerine/Threonine Kinase Assay
Assay EntryBiochemical phospho-specific activity (no antibody)
Excitation/Emission405/465, 535 nm
Format384-well Plate
For Use With (Application)FRET
Label or DyeMethylcoumarin
No. of Reactions800 Reactions
Quantity800 x 20 μL Assays
ReadoutEnd Point
ReferencesBiochem J. vol 296, pp. 843-9 (1993), J. Biol Chem, vol. 260, pp 14471-6 (1985)
Shipping ConditionDry Ice
SourceSer 7 Glycogen Synthase (KKLNRTLSVA) Based
Substrate PropertiesPeptide-Based Substrate
Target EntryMAPK8, MAPKAPK2, MAPKAPK5, CAMK2D, MAPK9, STK22B, STK22D, CAMK2A, MAPK14, MAPK10, MAPKAPK3
Target EnzymeSerine/Threonine Kinases
Validated KinasesMAPKAPK2, MAPKAPK3, MAPKAPK5, STK22B, STK22D, CAMK2A, CAMK2D, MAPK8, MAPK9, MAPK10, MAPK14
Unit SizeEach
Contents & Storage
Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.