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Thermo Scientific Biotin-11-dUTP (biotin-epsilon-aminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5'-triphosphate]) is supplied as 1 mM aqueous solution titrated to pH 7.0 with NaOH and is designed for enzymatic non-radioactive labelling of DNA.
Applications
• Enzymatic non-radioactive labeling of DNA by PCR
• Nick-translation
• cDNA synthesis
• Random primed labeling
• Primer extension
Biotin-11-dUTP can be enzymatically incorporated into DNA with:
• Reverse transcriptases
• Taq DNA Polymerase
• phi29 DNA Polymerase
• Klenow Fragment, exo
• Klenow Fragment
• DNA Polymerase I