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Citations & References (64)
Invitrogen™
TC-FlAsH™ II In-Cell Tetracysteine Tag Detection Kit (Green Fluorescence), for live-cell imaging
TC-FlAsH™ II In-Cell Tetracysteine Tag Detection Kit contains an expression tag-based fluorescence labeling reagent for live-cell labeling. Mammalian cell linesRead more
| Catalog Number | Quantity |
|---|---|
| T34561 | 1 Kit |
Catalog number T34561
Price (CNY)
13,588.00
飞享价
Ends: 31-Dec-2025
18,302.00Save 4,714.00 (26%)
1 kit
Quantity:
1 Kit
Price (CNY)
13,588.00
飞享价
Ends: 31-Dec-2025
18,302.00Save 4,714.00 (26%)
1 kit
TC-FlAsH™ II In-Cell Tetracysteine Tag Detection Kit contains an expression tag-based fluorescence labeling reagent for live-cell labeling. Mammalian cell lines expressing a protein fused to a tetracysteine tag (CCPGCC) can be labeled with the green-fluorescent FlAsH-EDT2 reagent contained in the kit. The tagged protein is fluorescent only when the labeling reagent is added. BAL wash buffer replaces the previously supplied Disperse Blue 3 and EDT-based wash buffer as an olfactorily more agreeable reagent that yields superior signal to noise.
The kit contains FlAsH-EDT2 labeling reagent (store at -20°C, protected from light) and BAL wash buffer (stored at 4°C). When stored as directed, the kit is stable for 6 months.
The kit contains FlAsH-EDT2 labeling reagent (store at -20°C, protected from light) and BAL wash buffer (stored at 4°C). When stored as directed, the kit is stable for 6 months.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
AssayTetracysteine Tag Detection
ColorGreen
Detection MethodFluorescence
For Use With (Equipment)Fluorescence Microscope
Label or DyeFlAsH
Product LineTC-FlAsH II
Product TypeTetracysteine Tag Detection Kit
Quantity1 Kit
Shipping ConditionWet Ice
FormatKit
Unit Size1 kit
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.
Citations & References (64)
Citations & References
Abstract
Resolution of de novo HIV production and trafficking in immature dendritic cells.
Journal:Nat Methods
PubMed ID:18059278
'The challenge in observing de novo virus production in human immunodeficiency virus (HIV)-infected dendritic cells (DCs) is the lack of resolution between cytosolic immature and endocytic mature HIV gag protein. To track HIV production, we developed an infectious HIV construct bearing a diothiol-resistant tetracysteine motif (dTCM) at the C terminus
Dynamic fluorescent imaging of human immunodeficiency virus type 1 gag in live cells by biarsenical labeling.
Journal:J Virol
PubMed ID:15767407
'Human immunodeficiency virus type 1 (HIV-1) Gag is the primary structural protein of the virus and is sufficient for particle formation. We utilized the recently developed biarsenical-labeling method to dynamically observe HIV-1 Gag within live cells by adding a tetracysteine tag (C-C-P-G-C-C) to the C terminus of Gag in both
Identification of an intracellular trafficking and assembly pathway for HIV-1 gag.
Journal:Traffic
PubMed ID:16683918
'Retroviral Gag proteins are membrane-bound polyproteins that are necessary and sufficient for virus-like particle (VLP) formation. It is not known how Gag traffics through the cell or how the site of particle production is determined. Here we use two techniques, biarsenical/tetracysteine (TC) labeling and release from a cycloheximide block, to
Real-time visualization of HIV-1 GAG trafficking in infected macrophages.
Journal:PLoS Pathog
PubMed ID:18369466
'HIV-1 particle production is driven by the Gag precursor protein Pr55(Gag). Despite significant progress in defining both the viral and cellular determinants of HIV-1 assembly and release, the trafficking pathway used by Gag to reach its site of assembly in the infected cell remains to be elucidated. The Gag trafficking
Correlated three-dimensional light and electron microscopy reveals transformation of mitochondria during apoptosis.
Journal:Nat Cell Biol
PubMed ID:17721514
'In addition to their role in cellular bioenergetics, mitochondria also initiate common forms of programmed cell death (apoptosis) through the release of proteins such as cytochrome c from the intermembrane and intracristal spaces. The release of these proteins is studied in populations of cells by western blotting mitochondrial and cytoplasmic