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CIAP（小牛肠碱性磷酸酶），1 U/μL
Calf Intestinal Alkaline Phosphatase (CIAP) is a phosphomonoesterase that removes 3´ and 5´ phosphates from DNA and RNA.Concentration: 1 unit/μLApplications: 了解更多信息
货号 18009027
价格（CNY)/ 1,000 units
价格649.00
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价格（CNY)/ 1,000 units
价格649.00
CIAP（小牛肠碱性磷酸酶），1 U/μL
货号18009027
价格（CNY)/ 1,000 units
价格649.00
-
Calf Intestinal Alkaline Phosphatase (CIAP) is a phosphomonoesterase that removes 3´ and 5´ phosphates from DNA and RNA.
Concentration: 1 unit/μL
Applications: Dephosphorylation of 5´-phosphorylated termini of vector DNA to prevent self-ligation (1). Dephosphorylation of 5´ termini of nucleic acids prior to forward reaction with kinase.
Source: Purified from calf intestinal mucosa.
Performance and Quality Testing: Endodeoxyribonuclease, 3´ exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay.
Unit Definition: One unit hydrolyzes 1 μmol of 4-nitrophenyl phosphate in 1 min. at 37°C.
Unit Reaction Conditions: 1 M diethanolamine buffer, 10 mM 4-nitrophenyl phosphate, 0.25 mM MgCl2 (pH 9.8) in 900 μl for 10 min. at 37°C.
Concentration: 1 unit/μL
Applications: Dephosphorylation of 5´-phosphorylated termini of vector DNA to prevent self-ligation (1). Dephosphorylation of 5´ termini of nucleic acids prior to forward reaction with kinase.
Source: Purified from calf intestinal mucosa.
Performance and Quality Testing: Endodeoxyribonuclease, 3´ exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay.
Unit Definition: One unit hydrolyzes 1 μmol of 4-nitrophenyl phosphate in 1 min. at 37°C.
Unit Reaction Conditions: 1 M diethanolamine buffer, 10 mM 4-nitrophenyl phosphate, 0.25 mM MgCl2 (pH 9.8) in 900 μl for 10 min. at 37°C.
仅供科研使用。不可用于诊断程序。
规格
浓度1 U/μL
运输条件经批准可置于湿冰或干冰上运输
酶CIP
兼容缓冲液二乙醇胺缓冲液
数量1000 U
产品类型碱性磷酸酶
Unit Size1,000 units
内容与储存
小牛肠碱性磷酸酶（1 单位/µL）配备一瓶 10X 去磷酸化缓冲液 [500 mM Tris-HCl（pH 值 8.5）、1 mM EDTA] 和一瓶稀释缓冲液 [50% 甘油、25 mM Tris-HCl（pH 值 7.6）、1 mM MgCl2 和 0.1 mM ZnCl2]。小牛肠碱性磷酸酶储存在 -20°C 下。