DNA Polymerase I/DNase I is an optimized mixture of both enzymes for efficient nick translation of DNA. Application: Labeling DNA with either radiolabeled or biotinylated nucleotides. Source: DNase I is purified from bovine pancreas; DNA Polymerase I from E. coli λ lysogen NM 964. Performance and Quality Testing: Performance tested in nick translation reaction. Unit Definition: One unit of DNA Polymerase I in the absence of DNase I incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template•primer. Unit Reaction Conditions: 50 mM potassium phosphate (pH 7.5), 6.7 mM MgCl2 , 1 mM 2-mercaptoethanol, 80 μg/ml template•primer, 32 μM dTTP, 69 nM [3H]dTTP, and enzyme in 100 μl for 30 min. at 37°C.
For Research Use Only. Not for use in diagnostic procedures.
规格
描述
DNA 聚合酶
聚合酶
DNA 聚合酶 I
外切核酸酶活性
3' - 5',5' - 3'
数量
250 U
运输条件
湿冰
热启动
否
Unit Size
250 units
内容与储存
DNA 聚合酶 I/DNase I 每微升含有 0.5 单位 DNA 聚合酶 I 和 0.4 毫单位 DNase I。储存在 -20°C 下。