Thermo Scientific Pierce SIA is a very short (1.5 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.
Features of SIA:
• Reactive groups:NHS ester and iodoacetyl • Reactive toward: amino and sulfhydryl groups • Shortest sulfhydryl-reactive and amine-reactive heterobifunctional crosslinker available; compare to SIAB • NHS ester reacts with primary amines at pH 7-9 to form a stable amide bond • Iodoacetyl group reacts with sulfhydryl groups at pH > 7.5 to form stable thioether bond • Non-cleavable • Water-insoluble (dissolve first in DMF or DMSO)
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
For Research Use Only. Not for use in diagnostic procedures.