Thermo Scientific Pierce TSAT is tris-(succinimidyl) aminotriacetate, a novel trifunctional crosslinker that contains three short spacers arms linked to a central nitrogen atom, each ending in an amine-reactive NHS-ester group.
Features of TSAT:
• Reactive groups:NHS ester (three ends) • Reactive towards: amino groups (primary amines) • NHS ester couples to amines at pH 7-9 form stable amide bonds • Trifunctional amine-reactive crosslinking agent • Non-cleavable • Water-insoluble (dissolve first in DMF or DMSO)
Applications: • Preparation of multicomponent aggregates • Polyerization of protein interactions for nearest-neighbor studies • Core molecule for the construction of dendritic polymers
Product References: Crosslinker Application Guide -- search for recent literature references for this product