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Pierce™ F(ab')2 微型制备试剂盒
The Thermo Scientific Pierce F(ab')2 Micro Preparation Kit uses immobilized pepsin protease to selectively digest whole human and other IgG 了解更多信息
货号 44688
价格（CNY)/ 1 kit
价格7,097.00
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价格（CNY)/ 1 kit
价格7,097.00
Pierce™ F(ab')2 微型制备试剂盒
货号44688
价格（CNY)/ 1 kit
价格7,097.00
-
The Thermo Scientific Pierce F(ab')2 Micro Preparation Kit uses immobilized pepsin protease to selectively digest whole human and other IgG antibodies to make F(ab')2 fragments that retain antigen binding activity.
Features of the F(ab')2 Micro Preparation Kit:
• Ideal when sample size is limited—Use the micro kit for 25 to 250 μg IgG.
• Enzyme-free digestion products—Immobilized Pepsin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the proteolytic enzyme
• Suitable for human and other species of IgG—the kit procedure is optimized for human, rabbit and mouse IgG. Pepsin-based digestion is effective for other species and subclasses of IgG, although the purification step requires that the antibody effectively binds to Protein A. (Note: for best results with mouse IgG1, use Part No. 44980.)
• Complete—kits include all reagents needed to prepare and purify antibody fragments
• Fast—spin format greatly reduces sample processing time
• Flexible—protocols are included for multiple species and IgG subclasses, as well as sample size and concentration.
• Efficient—enhanced yield and sample purity
These F(ab')2 Preparation Kits are suitable for human, rabbit, mouse and other species and subclasses of IgG except mouse IgG1. The antibody digestion reaction with pepsin agarose is performed in convenient disposable spin columns that allow efficient removal of the immobilized protease and maximum recovery of the IgG fragments. Also included in the kits are Thermo Scientific NAb Protein A Spin Columns and buffers to efficiently purify the resulting fragments. Protein A binds the Fc fragments and undigested IgG, allowing the pure F(ab')2 fragments to be recovered in the flow-through fraction. The kits also include Thermo Scientific Zeba Desalting Spin Columns for preparing the IgG sample quickly without dilution instead of utilizing time-consuming dialysis steps.
The kits use pepsin, a nonspecific endopeptidase, to enzymatically digest the Fc portion of whole IgG to yield the fragment known as F(ab')2. This fragment is composed of a pair of Fab' units connected by two disulfide bonds. Because the pepsin protease is supplied in immobilized form as beaded agarose resin, the digestion reaction is easily stopped by removing the resin from the IgG solution; the result is digest products that are enzyme-free.
Related Products
Pierce™ F(ab')2 Preparation Kit
Pierce™ Fab Micro Preparation Kit
Pierce™ Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit
Features of the F(ab')2 Micro Preparation Kit:
• Ideal when sample size is limited—Use the micro kit for 25 to 250 μg IgG.
• Enzyme-free digestion products—Immobilized Pepsin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the proteolytic enzyme
• Suitable for human and other species of IgG—the kit procedure is optimized for human, rabbit and mouse IgG. Pepsin-based digestion is effective for other species and subclasses of IgG, although the purification step requires that the antibody effectively binds to Protein A. (Note: for best results with mouse IgG1, use Part No. 44980.)
• Complete—kits include all reagents needed to prepare and purify antibody fragments
• Fast—spin format greatly reduces sample processing time
• Flexible—protocols are included for multiple species and IgG subclasses, as well as sample size and concentration.
• Efficient—enhanced yield and sample purity
These F(ab')2 Preparation Kits are suitable for human, rabbit, mouse and other species and subclasses of IgG except mouse IgG1. The antibody digestion reaction with pepsin agarose is performed in convenient disposable spin columns that allow efficient removal of the immobilized protease and maximum recovery of the IgG fragments. Also included in the kits are Thermo Scientific NAb Protein A Spin Columns and buffers to efficiently purify the resulting fragments. Protein A binds the Fc fragments and undigested IgG, allowing the pure F(ab')2 fragments to be recovered in the flow-through fraction. The kits also include Thermo Scientific Zeba Desalting Spin Columns for preparing the IgG sample quickly without dilution instead of utilizing time-consuming dialysis steps.
The kits use pepsin, a nonspecific endopeptidase, to enzymatically digest the Fc portion of whole IgG to yield the fragment known as F(ab')2. This fragment is composed of a pair of Fab' units connected by two disulfide bonds. Because the pepsin protease is supplied in immobilized form as beaded agarose resin, the digestion reaction is easily stopped by removing the resin from the IgG solution; the result is digest products that are enzyme-free.
Related Products
Pierce™ F(ab')2 Preparation Kit
Pierce™ Fab Micro Preparation Kit
Pierce™ Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit
仅供科研使用。不可用于诊断程序。
规格
描述Pierce F(ab')2 微型制备试剂盒
数量10份抗体样品，每份含 25 至 250µg IgG
产品线Pierce™
靶标基因ERK5/BMK1 (pan)
Unit Size1 kit
内容与储存
足够用于：10 份抗体样品，每份含 25 至 250 µg IgG
• Pierce 胃蛋白酶琼脂糖，0.5 mL
•，F(ab')2 酶切缓冲液，55 mL
• NAb 蛋白 A Plus 离心柱，0.2 mL，2 根柱
• PBS 包（每个可制备 500 mL），2 包
• IgG 洗脱缓冲液，50 mL
• Zeba 脱盐离心柱，7K MWCO，0.5 mL，10 根柱
• 离心柱，0.8 mL，10 根柱
• 微量离心管，2 mL，30 个管
储存在 4°C 下
• Pierce 胃蛋白酶琼脂糖，0.5 mL
•，F(ab')2 酶切缓冲液，55 mL
• NAb 蛋白 A Plus 离心柱，0.2 mL，2 根柱
• PBS 包（每个可制备 500 mL），2 包
• IgG 洗脱缓冲液，50 mL
• Zeba 脱盐离心柱，7K MWCO，0.5 mL，10 根柱
• 离心柱，0.8 mL，10 根柱
• 微量离心管，2 mL，30 个管
储存在 4°C 下