ExoSAP-IT™ Express PCR 纯化产品
ExoSAP-IT&trade; <i>Express</i> PCR 纯化产品
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Applied Biosystems™

ExoSAP-IT™ Express PCR 纯化产品

只需 5 分钟,ExoSAP-IT™ Express PCR Product Cleanup Reagent 即可从 PCR了解更多信息
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货号描述
75001.40.ULExoSAP-IT™ Express PCR Product Clean-up Reagent, 20 Reactions, Recombinant, Dry Ice, Removes Excess Primers and Unincorporated Nucleotides from a PCR Reaction, Use with PCR Purification
75001.4X.1.MLExoSAP-IT™ PCR Product Clean-up Reagent,2000 次反应,重组,干冰,去除 PCR 反应中多余的引物和未结合的核苷酸,可与 PCR 纯化配合使用,储存温度为 -5° 至 30°C
75001.1.EAExoSAP-IT™ PCR Product Clean-up Reagent,480 次反应,重组,干冰,去除 PCR 反应中多余的引物和未结合的核苷酸,可与 PCR 纯化配合使用,储存温度为 -5° 至 30°C
75001.10.MLExoSAP-IT™ PCR Product Cleanup Reagent,5000 次反应,重组,干冰,去除 PCR 反应中多余的引物和未结合的核苷酸,可与 PCR 纯化配合使用,储存温度为 -5° 至 30°C
75001.200.ULExoSAP-IT™ PCR Product Clean-up Reagent,100 次反应,重组,干冰,去除 PCR 反应中多余的引物和未结合的核苷酸,可与 PCR 纯化配合使用,储存温度为 -5° 至 30°C
75001.1.MLExoSAP-IT™ PCR Product Clean-up Reagent,500 次反应,重组,干冰,去除 PCR 反应中多余的引物和未结合的核苷酸,可与 PCR 纯化配合使用,储存温度为 -5° 至 30°C
货号 75001.40.UL
价格(CNY)/ 40 µL
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描述:
ExoSAP-IT™ Express PCR Product Clean-up Reagent, 20 Reactions, Recombinant, Dry Ice, Removes Excess Primers and Unincorporated Nucleotides from a PCR Reaction, Use with PCR Purification
只需 5 分钟,ExoSAP-IT™ Express PCR Product Cleanup Reagent 即可从 PCR 反应中除去过量的引物和未结合的核苷酸。与替代纯化方法(如离心柱或微珠)相比,该酶法纯化方法是一种更精确、产量更高和周转时间更快的方法。

• Remove unincorporated primers and nucleotides in about 5 minutes
• Prepare DNA sequencing samples with one pipetting step
• Conserve precious PCR products with up to 100% recovery, regardless of amplicon length
• Minimize spin column or bead cleanup

ExoSAP-IT Express PCR Product Cleanup Reagent consists of two hydrolytic enzymes: a novel exonuclease I and shrimp alkaline phosphatase (SAP), in a specially formulated buffer. 反应设置仅需一个移液步骤即可完成,其后是两次短暂孵育。第一次孵育可以水解过量的引物并使核苷酸去磷酸化。第二次高温孵育在约一分钟内完全不可逆地灭活酶。将 ExoSAP-IT Express 试剂直接加入 PCR 产物中,可省略向管、孔或柱的转移步骤并节省 PCR 扩增子,从而有助于降低交叉污染的可能性;此外,也无需进一步处理。样品即可用于 DNA 测序或 SNP 分析。

仅供研究使用。不可用于诊断程序。
规格
描述ExoSAP-IT™ Express PCR Product Clean-up Reagent, 20 Reactions, Recombinant, Dry Ice, Removes Excess Primers and Unincorporated Nucleotides from a PCR Reaction, Use with PCR Purification
产品规格8联管, 96孔板, 单管
反应次数20 Reactions
测试时间5 min。
Unit Size40 µL
内容与储存
Shipped on dry ice. Store at -20°C.