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Pierce™ 小鼠胚胎成纤维细胞分离试剂盒
The Thermo Scientific Pierce Mouse Embryonic Fibroblast Isolation Kit provides isolation and culturing reagents for the optimal yield and viability 了解更多信息
货号 88279
价格（CNY)/ 1 L
价格4,996.00
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价格（CNY)/ 1 L
价格4,996.00
Pierce™ 小鼠胚胎成纤维细胞分离试剂盒
货号88279
价格（CNY)/ 1 L
价格4,996.00
-
The Thermo Scientific Pierce Mouse Embryonic Fibroblast Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of fibroblasts from mouse embryos.
Features of the Mouse Embryonic Fibroblast Isolation Kit:
• Optimized – procedure and reagents optimized for viability, yield, purity, and ease-of-use
• Yield – provides a 2-fold increase in yield compared to do-it-yourself methods and other kits
• Functional – cultured fibroblasts express the appropriate biochemical markers
Includes:
• Complete kit includes Fibroblast Isolation Enzyme, Hanks' Balanced Salt Solution (HBSS), and DMEM for Primary Cell Isolation
The Mouse Embryonic Fibroblast Isolation Kit has been designed to obtain high viability and purity of embryonic fibroblasts using validated reagents and an optimized procedure. The kit contains a tissue-specific dissociation enzyme and media formulated for primary cell culture that promotes the isolation growth of functional fibroblasts. In addition, MEF isolated and cultured with the Pierce MEF Isolation Kit can be cryopreserved for future use with very minimal loss of viability.
Applications:
• Generation of immortalized cell lines from transgenic mice
• Feeder layer for stem cells
• IHC and IF studies
Because it uses an optimized tissue-specific dissociation enzyme, this kit isolates fibroblasts with greater yield and cell viability than cells isolated by existing methods. Isolated MEF express fibroblast-specific protein markers, confirming their viability and unperturbed metabolic function. After isolation, the mouse fibroblasts may be immortalized by continuous passage for the establishment of unique cell lines. They may also be used as a cell culture model for a diverse range of studies such as gene regulation and stem cell research [1,2,3,4].
More Product Data
• An improved method for highly efficient isolation of primary mouse embryonic fibroblasts
Related Products
Mouse Embryonic Fibroblast Isolation Enzyme 1 (with papain) for Pierce™ Primary Cell Isolation Kits
Features of the Mouse Embryonic Fibroblast Isolation Kit:
• Optimized – procedure and reagents optimized for viability, yield, purity, and ease-of-use
• Yield – provides a 2-fold increase in yield compared to do-it-yourself methods and other kits
• Functional – cultured fibroblasts express the appropriate biochemical markers
Includes:
• Complete kit includes Fibroblast Isolation Enzyme, Hanks' Balanced Salt Solution (HBSS), and DMEM for Primary Cell Isolation
The Mouse Embryonic Fibroblast Isolation Kit has been designed to obtain high viability and purity of embryonic fibroblasts using validated reagents and an optimized procedure. The kit contains a tissue-specific dissociation enzyme and media formulated for primary cell culture that promotes the isolation growth of functional fibroblasts. In addition, MEF isolated and cultured with the Pierce MEF Isolation Kit can be cryopreserved for future use with very minimal loss of viability.
Applications:
• Generation of immortalized cell lines from transgenic mice
• Feeder layer for stem cells
• IHC and IF studies
Because it uses an optimized tissue-specific dissociation enzyme, this kit isolates fibroblasts with greater yield and cell viability than cells isolated by existing methods. Isolated MEF express fibroblast-specific protein markers, confirming their viability and unperturbed metabolic function. After isolation, the mouse fibroblasts may be immortalized by continuous passage for the establishment of unique cell lines. They may also be used as a cell culture model for a diverse range of studies such as gene regulation and stem cell research [1,2,3,4].
More Product Data
• An improved method for highly efficient isolation of primary mouse embryonic fibroblasts
Related Products
Mouse Embryonic Fibroblast Isolation Enzyme 1 (with papain) for Pierce™ Primary Cell Isolation Kits
仅供科研使用。不可用于诊断程序。
规格
产品类型小鼠胚胎成纤维细胞分离试剂盒
细胞类型成纤维细胞
目标种属小鼠
产品线Pierce™
适用于（应用）干细胞、免疫组织化学技术和免疫荧光
足够用于50 份样品
数量1 个试剂盒
Unit Size1 L
内容与储存
足够用于：从50个胚胎小鼠体细胞组织中分离 MEF
• 用于原代细胞分离的 DMEM，500 mL
• MEF 分离酶（含木瓜蛋白酶），5瓶
• Hanks 平衡盐溶液（HBSS，不含 Ca2+ 和 Mg2+），500 mL
储存于 4°C。
• 用于原代细胞分离的 DMEM，500 mL
• MEF 分离酶（含木瓜蛋白酶），5瓶
• Hanks 平衡盐溶液（HBSS，不含 Ca2+ 和 Mg2+），500 mL
储存于 4°C。