用于 Illumina™ 系统的 Collibri™ 链式 RNA 文库制备试剂盒及人/小鼠/大鼠 rRNA 去除试剂盒和 UD 索引

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用于 Illumina™ 系统的 Collibri™ 链式 RNA 文库制备试剂盒及人/小鼠/大鼠 rRNA 去除试剂盒和 UD 索引

引用和文献 (4)

Invitrogen™

用于 Illumina™ 系统的 Collibri™ 链式 RNA 文库制备试剂盒及人/小鼠/大鼠 rRNA 去除试剂盒和 UD 索引

Invitrogen Collibri 链式 RNA 文库制备试剂盒（用于 Illumina 系统）设计用于在 Illumina了解更多信息

货号	反应次数	包括
A38996096	96 Reactions	RNA Prep and UD Index

货号 A38996096

价格（CNY)

反应次数:

96 Reactions

RNA Prep and UD Index

请求批量或定制报价

Invitrogen Collibri 链式 RNA 文库制备试剂盒（用于 Illumina 系统）设计用于在 Illumina 下一代测序 (NGS) 系统上进行链特异性 RNA 测序，以实现稳定的 cDNA 文库构建。试剂盒汇集了 SuperScript IV 逆转录酶、Dynabeads 磁粒子和 Platinum SuperFi DNA 聚合酶的卓越特点，使用户可以获得高质量的测序可用的文库

Collibri 链式 RNA 文库制备试剂盒可提供：
•适合自动化的短工作流程 — 总 RNA 可在6.5小时内转化为测序可用的文库
• 试剂中的惰性染料 — 显示文库生成进度而对质量没有任何不利影响
• 出色的 rRNA 去除
• 均一的转录本覆盖、高灵敏度的转录本检测
•高灵敏度的差别基因表达检测
• >98% 链特异性
• 非编码 RNA 的高效检测
• 3‘ 末端序列信息的保留

此 Collibri 链式 RNA 文库制备试剂盒设计用于以起始上样量为 100 ng—1 µg 的总人、小鼠或大鼠 RNA 快速、方便地制备文库。单管接头连接和逆转录反应以及磁珠法 rRNA 去除和文库纯化步骤使整个工作流程可在约 6.5 小时内完成。该试剂盒适用于各种质量 RNA 样品，包括 FFPE 样品。

过程完整性的视觉提示
为了实现极大的便利性，在整个文库制备工作流程中包括过程完整性的视觉提示。使用关键试剂中染料的视觉反馈监测文库生成进度。反应混合物在每个步骤中添加关键组分的反应颜色改变，以确保文库制备具有较高的成功率。惰性染料不会干扰酶促反应且不影响测序结果。

工作原理
Collibri 链式 RNA 文库制备试剂盒及人/小鼠/大鼠 rRNA 去除试剂盒包括核糖体 RNA (rRNA) 去除试剂，通过出色去除人、小鼠或大鼠 rRNA 获得对转录组的全面了解。通过使用 Platinum SuperFi DNA 聚合酶的 PCR 扩增提供全长 Illumina 兼容接头，以构建兼容单端或双端测序的单索引或独特的双索引文库。该试剂盒含有 24 或 96 个带条形码的单索引 PCR 引物 (i7)，预混合通用 i5 引物或独特的双索引 PCR 引物，可多路复用多达 96 个文库。优化的纯化步骤能够高效去除残留引物和接头/引物二聚体，同时保留高文库产量。

在进行测序之前，建议使用相关产品 Collibri 文库定量试剂盒进行基于 qPCR 的文库定量。

应用
建议将 Collibri 链式 RNA 文库制备试剂盒用于：
•基因表达研究
•选择性剪接分析
•非编码 RNA 检测和发现
•选择性聚腺苷酸化位点的鉴别
•基因融合检测
•转录本亚型检测

仅供科研使用。不可用于诊断程序。

描述The product contains modules A39117096, A39119196, A39114096

适用于（设备）Illumina NGS 仪器

包括RNA Prep and UD Index

库cDNA 文库

反应次数96 Reactions

产品线Collibri

数量96 reactions

测序类型转录组测序

运输条件经批准可置于湿冰或干冰上运输

工作流程步骤文库生成

样品类型RNA

Unit SizeEach

内容与储存

• Collibri 文库制备试剂盒，在 -20°C 下储存
• Collibri 文库纯化试剂盒，在 4°C 下储存

常见问题解答 (FAQ)

Is the elution buffer, included in the Collibri Stranded RNA Library Prep Kit for Illumina Systems, "Tris buffered" or "Tris-Tween"?

Collibri Stranded RNA Library Prep Kit for Illumina Systems elution buffer is based on Tris-HCl, with no EDTA or EGTA.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Is there an RNA control for optimizing fragment length included with the Collibri Stranded RNA Library Prep Kit for Illumina Systems?

The Collibri Stranded RNA Library Prep Kit for Illumina Systems does not include an RNA control. Recommendations for RNA controls can be found in the Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina User Guide, page 16 "Guidelines for RNA Controls".

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the recommended RNA input sample types and respective sample amounts for Collibri Stranded RNA Library Prep Kit for Illumina Systems?

Information for RNA sample type and amount can be found in the Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina User Guide, page 15 "Guidelines for RNA sample type and amount".

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the differences between all Collibri Stranded RNA Library Prep Kit for Illumina Systems catalog numbers?

Information regarding the different catalog numbers can be found in the Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina User Guide, page 3.
The different catalog numbers allow for the option to purchase different sizes of the library kit and with or without Unique Dual Indexes.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What additional materials are required when using Collibri Stranded RNA Library Prep Kit for Illumina Systems?

A list of required materials that are not supplied with the kit can be found in the Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina User Guide, page 11-12.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

引用和文献 (4)

引用和文献

Abstract

Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease.

Authors:Karvelis T, Druteika G, Bigelyte G, Budre K, Zedaveinyte R, Silanskas A, Kazlauskas D, Venclovas C, Siksnys V

Journal:Nature

PubMed ID:34619744

'Transposition has a key role in reshaping genomes of all living organisms'

Evaluation of cisplatin-induced injury in human kidney organoids.

Authors:Digby JLM, Vanichapol T, Przepiorski A, Davidson AJ, Sander V

Journal:Am J Physiol Renal Physiol

PubMed ID:32150447

Acute kidney injury (AKI) remains a major global healthcare problem, and there is a need to develop human-based models to study AKI in vitro. Toward this goal, we have characterized induced pluripotent stem cell-derived human kidney organoids and their response to cisplatin, a chemotherapeutic drug that induces AKI and preferentially ... More

In situ approaches show the limitation of the spoilage potential of Juniperus phoenicea L. essential oil against cold-tolerant Pseudomonas fluorescens KM24.

Authors:Myszka K, Tomas N, Wolko L, Szwengiel A, Grygier A, Nuc K, Majcher M

Journal:Appl Microbiol Biotechnol

PubMed ID:33988734

The present study aimed to elucidate the effect of subinhibitory concentrations (sub-MICs) of juniper essential oil (EO), a-pinene, and sabinene on the quorum-sensing (QS)-mediated proteolytic and lipolytic properties of Pseudomonas fluorescens KM24. These activities were verified under in situ conditions, in which sub-MICs of the agents altered the morphology of ... More

TDP-43 prevents endogenous RNAs from triggering a lethal RIG-I-dependent interferon response.

Authors:Dunker W, Ye X, Zhao Y, Liu L, Richardson A, Karijolich J

Journal:Cell Rep

PubMed ID:33852834

RIG-I-like receptors (RLRs) are involved in the discrimination of self versus non-self via the recognition of double-stranded RNA (dsRNA). Emerging evidence suggests that immunostimulatory dsRNAs are ubiquitously expressed but are disrupted or sequestered by cellular RNA binding proteins (RBPs). TDP-43 is an RBP associated with multiple neurological disorders and is ... More