The Ion AmpliSeq Mouse TCR Beta SR Assay is a robust, targeted next-generation sequencing (NGS) assay designed to accurately identify and measure the clonal expansion of T lymphocytes by targeting the complementarity-determining region 3 (CDR3) of the T-cell receptor (TCR) gene locus from total RNA input. The assay can be used for basic and translational research to identify T-cell clones since the nucleotide sequence of the CDR3 region is unique to each T cell and codes for the part of the TCR beta chain that is involved in antigen recognition. For DNA samples please see Cat. No. A45488.

Benefits of the Ion AmpliSeq Mouse TCR Beta SR Assay, RNA, include:
• Compatibility with a vast array of research sample types, including FFPE tissue, fresh-frozen (FF) tissue, whole blood, PBLs, and PBMCs
• High sensitivity and low limit of detection (LoD) for rare clone identification through dual-barcode indexing
• Efficient workflow with 48 hour sample-to-results time
• Flexible input requirements ranging from 25 ng to 1 μg
• Streamlined and user-friendly informatics solution with with multi-sample analysis functionality

The Ion AmpliSeq Mouse TCR Beta SR Kit, RNA, provides a single pool of multiplex PCR primers and library reagents to generate 90-bp amplicons that can be sequenced on all chip types supported by the Ion GeneStudio S5 sequencing systems, allowing you to pick the best multiplexing configuration for your unique sample batching needs and throughput requirements. The entire workflow from library preparation to analysis of samples can be accomplished in two days using the Ion Chef templating system and Ion GeneStudio S5 system.

The Ion AmpliSeq Mouse TCR Beta SR RNA Assay, with its high sensitivity and specificity, supports key applications in the field of immunology, immuno-oncology, hemato-oncology, and vaccine research. The high-sensitivity dual-barcode indexing, flexible input requirements (100 ng–1 μg), high-depth sequencing, and high-throughput capability make this assay ideal for testing a variety of hypotheses for basic or translational biomarker research. A small fragment size requirement from the input materials for library creation and high multiplexing on the Ion 530 Chip of the Ion GeneStudio S5 sequencers enables researchers to focus on testing a variety of use cases in mouse models including the role of T cells in generating immune response to immunotherapies such as checkpoint blockade inhibitors, cancer vaccines, or chimeric antigen receptor (CAR) T-cell therapies.

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Note: Information about data analysis using Ion Reporter Software v5.12 can be found in the User Guide below.