CultureCEPT™ Supplement (1000X)

Gibco CultureCEPT Supplement reduces cellular stress and improves viability of stem cells, differentiated cells, and primary cells during a wide variety of handling and processing steps where cell damage and death can limit the success of a cell culture workflow. It is a unique formulation that provides superior cell protection compared to traditional ROCK inhibitors, such as Y-27632, and other commercially available cell culture reagents.

The formulation is based on advancements from the Stem Cell Translation Laboratory at the NIH's National Center for Advancing Translational Sciences (NCATS), where a screen of over 15,000 compounds identified a combination of small molecules (referred to as CEPT) that work together to provide unmatched cytoprotection in passaging pluripotent stem cells, embryoid body creation, organoid formation, single-cell cloning, gene-editing, differentiation, and recovery of cryopreserved cells including PSCs, cardiomyocytes, neurons, astrocytes, and hepatocytes. CultureCEPT Supplement contains the CEPT cocktail molecules, chroman 1, emricasan, polyamines, and trans-ISRIB, to provide improved cell viability.

Achieve unparalleled cell survival in numerous applications
CultureCEPT Supplement will benefit scientists looking for:
• Improved viability of PSCs, differentiated cells, or primary cells following recovery from cryopreservation
• Improved viability during the passaging of stem cells
• Improved viability during electroporation or transfection
• Improved clonal efficiency during gene-editing and clonal selection with PSCs
• Improved embryoid body and organoid formation

CultureCEPT Supplement harnesses the power of CEPT
• Innovative small molecules comprising the CEPT cocktail have been extensively tested by the NIH's NCATS (Chen et al., 2021)
• Growing number of publications highlights utility and exceptional performance of CEPT small molecules

Compatible with trusted Gibco media systems
• Evaluated in StemScale PSC Suspension Medium, StemFlex Medium, Essential 8 Medium, Essential 8 Flex Medium, Neurobasal Medium, and Neurobasal Plus Medium
• For over 60 years Gibco reagents have enabled successful cell culture

Ready-to-use
• Fully formulated 1000X
• Simply add directly to media
• No weighing, no resuspending, no displacing media, no hassle

Citations and references

Chen Y, et al. A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. Nat Methods. 2021 May;18(5):528-541. 

Tristan CA, et al. Efficient and safe single-cell cloning of human pluripotent stem cells using the CEPT cocktail. Nat Protoc. 2023 Jan;18(1):58-80. 

Ryu S, et al. Stress-free cell aggregation by using the CEPT cocktail enhances embryoid body and organoid fitness. Biofabrication. 2023 Dec;16(1):10.1088/1758-5090/ad0d13.

Deng T, et al. Scalable generation of sensory neurons from human pluripotent stem cells. Stem Cell Reports. 2023 Apr 11;18(4):1030-1047.

Jovanovic VM, et al. A defined roadmap of radial glia and astrocyte differentiation from human pluripotent stem cells. Stem Cell Reports. 2023 Aug;18(8):1701-1720.

McIntire E, et al. Guided Differentiation of Pluripotent Stem Cells for Cardiac Cell Diversity. Preprint. bioRxiv. 2024 Feb;2023.07.21.550072.

Jin M, et al. Generation of iPSC-based human-mouse microglial brain chimeras to study senescence of human microglia. STAR Protoc. 2022 Dec;3(4):101847.

Liu L, et al. Modeling post-implantation stages of human development into early organogenesis with stem-cell-derived peri-gastruloids. Cell. 2023 Aug;186(18):3776-3792.e16.

Kang SY, et al. A Pillar and Perfusion Plate Platform for Robust Human Organoid Culture and Analysis. Adv Healthc Mater. Published online August 24, 2023.

Deng C, et al. Massively parallel characterization of psychiatric disorder-associated and cell-type-specific regulatory elements in the developing human cortex. Preprint. bioRxiv. 2023 Feb;2023.02.15.528663.

Tristan CA, et al. Robotic high-throughput biomanufacturing and functional differentiation of human pluripotent stem cells. Stem Cell Reports. 2021 Dec 14;16(12):3076-3092.  

Ryu S, et al. Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small Molecules. Methods Mol Biol. 2022;2454:811-827. 

Held A, Adler M, Marques C, et al. iPSC motor neurons, but not other derived cell types, capture gene expression changes in postmortem sporadic ALS motor neurons. Cell Rep. 2023 Sep;42(9):113046.