CyQUANT™ NF 细胞增殖检测

引用和文献 (24)

Invitrogen™

CyQUANT™ NF 细胞增殖检测

Name: CyQUANT™ NF 细胞增殖检测
SKU: C35007
Price: 2902.00 CNY

CyQUANT™ NF 细胞增殖检测提供了在微孔板内对群体内细胞进行定量的快速灵敏方法。CyQUANT™ NF 细胞增殖检测的特点为：•比 MTT 或 AlamarBlue™ 测定试剂盒更灵敏•线性检测范围为每孔100至20,000个细胞了解更多信息

更改视图

货号	细胞类型	数量
C35007	NF Cell	200 Assays
C35011	Direct Cell	10 Microplates
C35013	Direct Red Cell	10块微孔板
C35012	Direct Cell	100 Microplates
C7026	For cells in culture	1000 次检测
C35006	NF Cell	1000 Assays
C7027	Cell Lysis Buffer	50 mL

7 选项

货号 C35007

价格（CNY)

2,902.00

Each

添加至购物车

细胞类型:

NF Cell

数量:

200 Assays

价格（CNY)

2,902.00

Each

添加至购物车

CyQUANT™ NF 细胞增殖检测提供了在微孔板内对群体内细胞进行定量的快速灵敏方法。

CyQUANT™ NF 细胞增殖检测的特点为：

•比 MTT 或 AlamarBlue™ 测定试剂盒更灵敏
•线性检测范围为每孔100至20,000个细胞（96孔微孔板）
•测定试剂盒可以在一小时内完成

快速简单的细胞增殖检测方法
CyQUANT™ NF 细胞增殖测定试剂盒不需要细胞裂解、长时间孵育、放射性标记或从细胞中去除染色剂。CyQUANT™ NF 检测无需进行原始 CyQUANT™ 细胞增殖检测的冻融细胞裂解步骤，代之以一种细胞通透性的 DNA 结合染料和一种质膜通透性的试剂。CyQUANT™ NF 细胞增殖测定试剂盒可以在96孔或384孔微孔板内使用，有两种包装形式：200次测定试剂盒 (C35007)，适用于较小样本量，以及1000次测定试剂盒 (C35006)，适用于高通量应用。

AlamarBlue™ 是 TREK Diagnostic Systems 公司的注册商标。

仅供科研使用。不可用于诊断程序。

规格

细胞类型NF Cell

检测方法荧光

染料类型其他标记或染料

激发/发射480/520 nm

产品规格384 孔板、96 孔板

数量200 Assays

运输条件室温

适用于（应用）增殖测定试剂盒

适用于（设备）微孔板读数仪, HTS Reader

产品线CyQUANT

产品类型细胞增殖测定试剂盒

Unit SizeEach

内容与储存

•CyQUANT™ NF dye reagent
•Dye delivery reagent
•5X HBSS buffer (Hank’s balanced salt solution)

It is possible to observe precipitate in HBSS buffer. The precipitate does not have an impact on the use of this kit, nor the results that are gathered from its use.

常见问题解答 (FAQ)

你们提供测定神经元细胞健康的产品吗？

可采用PrestoBlue细胞活力染色剂和CyQUANT细胞增殖检测试剂盒。我们还提供神经突生长染色试剂盒（货号A15001）。更多有关我们的神经元细胞健康检测试剂的信息请见此处（https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html）。

What is the difference between the original CyQuant assay and the CyQuant NF assay?

The original CyQUANT assay provides sensitive detection of cells over a 1000-fold linear dynamic range. In this assay, a freeze-thaw cell lysis step is required to facilitate the interaction of the CyQUANT GR dye with DNA. The CyQUANT NF assay avoids this freeze-thaw step by using a DNA binding dye in combination with a plasma membrane permeabilization reagent. The CyQUANT NF protocol requires only aspiration of growth medium (for adherent cells), replacement with dye binding solution, incubation for 30-60 minutes, and then measurement of fluorescence in a microplate reader. The assay is designed to produce a linear analytical response from at least 100-20,000 cells per well in most cell lines in a 96-well microplate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer any products to measure neuronal cell health?

PrestoBlue Cell Viability Stain and CyQUANT Cell Proliferation Assay Kit can be used. We also offer a Neurite Outgrowth Staining Kit (Cat. No. A15001). More information about our different assays for neuronal cell health can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (24)

引用和文献

Abstract

KIF14 messenger RNA expression is independently prognostic for outcome in lung cancer.

Authors:Corson TW,Zhu CQ,Lau SK,Shepherd FA,Tsao MS,Gallie BL

Journal:Clinical cancer research : an official journal of the American Association for Cancer Research

PubMed ID:17545527

Functional symbiosis between endothelium and epithelial cells in glomeruli.

Authors:Hirschberg R, Wang S, Mitu GM,

Journal:Cell Tissue Res

PubMed ID:17999087

'In some capillary beds, pericytes regulate endothelial growth. Capillaries with high filtration capacity, such as those in renal glomeruli, lack pericytes. Glomerular endothelium lies adjacent to visceral epithelial cells (podocytes) that are anchored to and cover the anti-luminal surface of the basement membrane. We have tested the hypothesis that podocytes ... More

Flex-Hets differentially induce apoptosis in cancer over normal cells by directly targeting mitochondria.

Authors:Liu T, Hannafon B, Gill L, Kelly W, Benbrook D

Journal:Mol Cancer Ther

PubMed ID:17575110

'Flex-Het drugs induce apoptosis in multiple types of cancer cells, with little effect on normal cells. This apoptosis occurs through the intrinsic mitochondrial pathway accompanied by generation of reactive oxygen species (ROS). The objective of this study was to determine if direct or indirect targeting of mitochondria is responsible for ... More

Cytosolic delivery of membrane-impermeable molecules in dendritic cells using pH-responsive core-shell nanoparticles.

Authors:Hu Y, Litwin T, Nagaraja AR, Kwong B, Katz J, Watson N, Irvine DJ,

Journal:Nano Lett

PubMed ID:17887715

'Polycations that absorb protons in response to the acidification of endosomes can theoretically disrupt these vesicles via the "proton sponge" effect. To exploit this mechanism, we created nanoparticles with a segregated core-shell structure for efficient, noncytotoxic intracellular drug delivery. Cross-linked polymer nanoparticles were synthesized with a pH-responsive core and hydrophilic ... More

Decrease of endogenous vascular endothelial growth factor may not affect glioma cell proliferation and invasion.

Authors:Hong X, Jiang F, Kalkanis SN, Zhang ZG, Zhang X, Zheng X, Mikkelsen T, Jiang H, Chopp M

Journal:J Exp Ther Oncol

PubMed ID:17552362

'Vascular endothelial growth factor (VEGF) is abundantly produced by glioma cells especially glioblastoma, the most malignant form of astrocytoma. VEGF, a well known angiogenic factor, acts in a paracrine fashion on endothelial cells to develop tumor vasculature. However, recent studies have found that several tumor cells express VEGF receptors, and ... More

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