Chargeswitch™ Pro 过滤柱收集管

The Chargeswitch™-Pro Filter Collection Tube is a 50 mL conical tube that will accommodate the centrifuge protocol of Chargeswitch™ Pro了解更多信息

货号	数量
CS32000	25 支管

货号 CS32000

价格（CNY)

384.00

添加至购物车

25 支管

价格（CNY)

384.00

添加至购物车

The Chargeswitch™-Pro Filter Collection Tube is a 50 mL conical tube that will accommodate the centrifuge protocol of Chargeswitch™ Pro Filter Plasmid Midi and Maxi kits.

仅供科研使用。不可用于诊断程序。

数量25 支管

运输条件室温

管类型收集管

Unit SizeEach

内容与储存

25 x 50 mL 锥形管。室温保存

常见问题解答 (FAQ)

使用ChargeSwitch试剂盒时我用水替代洗脱缓冲液，但是得到非常低的质粒产量。这是为什么？

我们强烈建议使用试剂盒提供的洗脱缓冲液，并且不建议用水进行洗脱。如果你需要使用任何其它的缓冲液进行洗脱，请确保使用pH 8.5–9.0的缓冲液以实现DNA的高效洗脱。

ChargeSwitch Pro试剂盒和Charge-Switch Pro filter试剂盒的区别是什么？

带filter的试剂盒是基于一个双巢式柱的设计，它能够有助于通过最快最简便的步骤获得高质量的质粒（ (<10 EU/µg DNA）。经过改良的缓冲液1可去除更多内毒素。

ChargeSwitch Pro Filter Plasmid Mini 或 Midi试剂盒纯化质粒的常规产量是多少？

通常而言ChargeSwitch Pro mini试剂盒从5mL菌液可得到25 µg质粒，midi试剂盒从25 mL菌液可得到200µg质粒，而 maxi试剂盒从100 mL菌液可得到800 µg质粒。试剂盒可以纯化的质粒大小范围通常是3–9 kb。

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.

What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

View all Chargeswitch™ Pro 过滤柱收集管 FAQs