GeneBLAzer™ER beta DA(Division Arrested) cells and ER beta-UAS-bla GripTite™ 293 cells contain the ligand-binding domain (LBD) of the human Estrogen了解更多信息
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货号 K1399
价格(CNY)/ 1 plate
价格16,619.00
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价格(CNY)/ 1 plate
价格16,619.00
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GeneBLAzer™ ER β DA 检测试剂盒
货号K1399
价格(CNY)/ 1 plate
价格16,619.00
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GeneBLAzer™ER beta DA(Division Arrested) cells and ER beta-UAS-bla GripTite™ 293 cells contain the ligand-binding domain (LBD) of the human Estrogen receptor beta (ER beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla GripTite™ 293 cell line. GeneBLAzer™UAS-bla GripTite™ 293 cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both ER beta DA cells and ER beta-UAS-bla GripTite™ 293 cells are functionally validated for Z' and EC50 concentrations of 17-beta Estradiol. In addition, ER beta-UAS-bla GripTite™ 293 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time. Additional testing data using alternate stimuli are also available.
For Research Use Only. Not for use in diagnostic procedures.
规格
交替受体激动剂胆酸、脱氧胆酸
拮抗剂(z)-没药甾酮
检测条目基于细胞的 β 内酰胺酶报告基因
细胞状态分裂停滞细胞
检测方法荧光
基因 ID (Entrez)2100
基因符号ERβ
主要受体激动剂鹅去氧胆酸 (CDCA)
产品线GeneBLAzer™
数量1 plate
报告基因BLA(β-内酰胺酶)
目标条目ER β、ESR2、ERB、NR3A2
细胞系293T (HEK)
系统类型GeneBLAzer™
成药靶标核受体
Unit Size1 plate
内容与储存
运输: 干冰储存:-80°C/液氮足够的分裂停滞细胞和底物以测定一个 384 孔板。包括:ER β HEK 293 T DA 细胞 (K1399A)LiveBLAzer™-FRET B/G 上样试剂盒,70 µg