细胞传感器™ CRE-bla FreeStyle™ 293F 细胞系
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Thermo Scientific™

细胞传感器™ CRE-bla FreeStyle™ 293F 细胞系

The CellSensor™ CRE-bla FreeStyle™ 293F cell line contains a beta-lactamase reporter gene under control of the CRE response element stably了解更多信息
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货号 K1636
价格(CNY)/ 1 vial
价格160,738.00
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价格(CNY)/ 1 vial
价格160,738.00
添加至购物车
细胞传感器™ CRE-bla FreeStyle™ 293F 细胞系
货号K1636
价格(CNY)/ 1 vial
价格160,738.00
-
添加至购物车

The CellSensor™ CRE-bla FreeStyle™ 293F cell line contains a beta-lactamase reporter gene under control of the CRE response element stably integrated into FreeStyle™ 293F cells. To construct this cell line, the CRE -bla construct was transduced into FreeStyle™ 293F cells by lentivirus. Flow cytometry was used to isolate cells responsive to forskolin. Assay development experiments performed on this cell line included DMSO tolerance, cell number, stimulation time and substrate loading time. Functional validation of this cell line under optimized conditions included and Z' and EC50 determinations using forskolin as a primary agonist. The CellSensor™ CRE-bla FreeStyle™ 293F cell line is responsive to forskolin and can be used to build specific G-protein coupled receptor (GPCR) assays after transfection of additional genes of interest or to detect changes in cellular cAMP levels. This cell line can be adapted for high-throughput screening for agonists or antagonists with compound libraries. Candidate drugs can be tested for dose response against this cell line. Academic and non-profit customers, please inquire for special pricing.

仅供科研使用。不可用于诊断程序。
规格
检测条目基于细胞的 β 内酰胺酶报告基因
细胞状态分裂细胞
产品线细胞传感器™
数量1 Vial
目标条目PKA、cAMP、cAMP/PKA 通路
系统类型CellSensor™
成药靶标信号通路
Unit Size1 vial
内容与储存
CellSensor™ 细胞系在干冰上运输。接收后应立即储存于液氮中,或解冻后立即使用。