CellSensor™ LEF/TCF-bla FreeStyle™ 293F 细胞系
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Invitrogen™

CellSensor™ LEF/TCF-bla FreeStyle™ 293F 细胞系

Wnt signaling via beta - catenin plays a central role in development and homeostasis. This pathway is invariably disrupted in了解更多信息
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货号 K1677
价格(CNY)/ 1 vial
价格164,759.00
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价格(CNY)/ 1 vial
价格164,759.00
添加至购物车
CellSensor™ LEF/TCF-bla FreeStyle™ 293F 细胞系
货号K1677
价格(CNY)/ 1 vial
价格164,759.00
-
添加至购物车
Wnt signaling via beta - catenin plays a central role in development and homeostasis. This pathway is invariably disrupted in colorectal tumors and commonly affected by mutation in other cancers. Binding of the Wnt protein to the Frizzled transmembrane receptors initiates a signaling cascade that activates the Dishevelled protein, which then inhibits the serine/threonine kinase Glycogen Synthase - 3 Beta (GSK - 3B). This signal leads to functional inactivation and dissociation of a multiprotein beta - catenin destruction complex made up of the tumor suppressor protein Adenomatous Polyposis Coli (APC), GSK - 3B, and a scaffold of Axin. This results in dephosphorylation and dissociation of beta - catenin. Unphosphorylated beta - catenin is stabilized and accumulates in the cytoplasm of the cell. Beta - catenin then associates with the Lymphoid Enhancer Factor (LEF)/T - Cell Factor (TCF) family of transcription factors in the nucleus, leading to transcription and expression of target genes, such as c - Myc, c - jun, Fra, and cyclin D1. The CellSensor® LEF/TCF - bla FreeStyle™ 293 Cell Line contains a beta - lactamase reporter gene under control of the LEF/TCF stably integrated into FreeStyle™ 293 cells. This cell line is validated for EC50 and Z' - factor under optimized conditions using mWnt3a. This cell line has also been tested under various experimental conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time. CellSensor® LEF/TCF - bla FreeStyle™ 293F cells were stimulated with mWnt3a over the indicated concentration range in a 384 - well format. Cells were incubated for 5 hrs with agonist and 0.5% DMSO and then combined with LiveBLAzer™ - FRET B/G Substrate (CCF4 - AM) for 2.5 hrs. Fluorescence emission values at 460 nm and 530 nm were obtained using a standard fluorescence plate reader. The 460/530 ratios are shown plotted against the indicated concentrations of mWnt3a. Academic and non-profit customers, please inquire for special pricing.
仅供科研使用。不可用于诊断程序。
规格
检测条目基于细胞的 β 内酰胺酶报告基因
细胞状态分裂细胞
产品线CellSensor™
数量1 vial
目标条目β-连环蛋白、Wnt/β-连环蛋白通路
系统类型CellSensor™
成药靶标信号通路
Unit Size1 vial
内容与储存
CellSensor™ 细胞系在干冰上运输。接收后应立即储存于液氮中,或解冻后立即使用。