LysoLight™ Antibody Labeling Kits
LysoLight™ Antibody Labeling Kits
Invitrogen™

LysoLight™ Antibody Labeling Kits

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LysoLight™ Deep Red Antibody Labeling Kits offer an efficient method for conjugating antibodies with LysoLight™ Deep Red dye. This process allows for specific and sensitive detection of internalized antibodies within lysosomes using fluorescence microscopy, flow cytometry, or high content imaging platforms.
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货号产品类型颜色数量
L36004Amine Reactive DyeDeep Red500 μg
L36001Antibody Labeling KitDeep Red3 x 100 μg
L36002Antibody Labeling KitDeep Red3 x 1 mg
L36003Amine Reactive DyeDeep Red3 x 100 μg
L36005Antibody Labeling KitGreen3 x 100 μg
L36006Antibody Labeling KitGreen3 x 1 mg
L36007Amine Reactive DyeGreen3 x 100 μg
L36008Amine Reactive DyeGreen500 μg
货号 L36004
价格(CNY)/ Each
价格7,689.00
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产品类型:
Amine Reactive Dye
颜色:
Deep Red
数量:
500 μg
价格(CNY)/ Each
价格7,689.00
添加至购物车
LysoLight™ Antibody Labeling Kits
货号L36004
价格(CNY)/ Each
价格7,689.00
-
添加至购物车
LysoLight Deep Red Antibody Labeling Kits provide a powerful tool for visualizing the lysosomal localization and catabolic degradation of antibodies and proteins. These kits utilize a unique fluorophore that is only active upon enzymatic cleavage by the lysosomal specific enzyme, Cathepsin B. Outside of the lysosome, LysoLight Deep Red has little to no observable background fluorescence, and only upon localization to the protease rich environment of the Lysosome, does LysoLight become activated and brightly fluoresce.

The labeling protocol provided in the kits involves a simple and rapid procedure, ensuring minimal hands-on time and reliable results. The kits include all necessary reagents, including the LysoLight Deep Red dye, buffers, and purification columns, to facilitate the antibody labeling process.

The LysoLight Deep Red SDP ester contained in these kits readily reacts with lysine residues in antibodies or proteins to yield a covalently attached fluorogenic sensor. The spin columns included in the kits are used for purifying the labeled antibody from excess dye with yields of 70–95%. The kits contain sufficient reagents for three labeling reactions of 100 μg or 1 mg of antibody. The user guide describes a general protocol for using the amine-reactive, SDP ester form of the LysoLight Deep Red dye for labeling either 100 μg or 1 mg of IgG.

仅供研究使用。
规格
颜色Deep Red
描述LysoLight™ Deep Red Amine Reactive Dye
检测方法荧光
适用于(应用)Antibody and Protein Labeling
适用于(设备)Flow Cytometer, Fluorescence Microscope, High Content Analysis Instrument
产品类型Amine Reactive Dye
环保功能Enhanced stability
产品线LysoLight™
数量500 μg
溶解度DMSO(二甲亚砜)
激发/发射650/668 nm
Unit SizeEach