Visualizing the distribution and transport of mRNAs in living cells.
Authors:Bratu DP, Cha BJ, Mhlanga MM, Kramer FR, Tyagi S
Journal:Proc Natl Acad Sci U S A
PubMed ID:14583593
We have visualized the movements of native mRNAs in living cells. Using nuclease-resistant molecular beacons, we imaged the transport and localization of oskar mRNA in Drosophila melanogaster oocytes. When the localization pattern was altered by genetic manipulation of the mRNA's 3' untranslated region, or by chemical perturbation of the intracellular ... More