The pPICZ A, B, & C Pichia Vectors are designed for simple cloning and selection, high-level expression, and rapid detection and purification of the recombinant protein. These vectors contain the Zeocin™ resistance gene for direct selection of multi-copy integrant strains. By selecting with increasing amounts of Zeocin™, strains with multiple copies of your gene of interest integrated into the genome are obtained. Increasing the number of copies of the gene of interest in a recombinant Pichia strain can result in higher expression levels. The vectors are included in the EasySelect™ Pichia Expression Kit (Cat. No. K1740-01).
Features of the pPICZ vectors include:
• Inducible AOX1 promoter for high-level expression in Pichia pastoris c-myc epitope tag for convenient detection with an Anti-myc Antibody • C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody • Zeocin™ resistance for direct selection of multi-copy integrants
For Research Use Only. Not for use in diagnostic procedures.
规格
促进剂
AOX1
产品类型
毕赤酵母表达载体
耐抗生素细菌
Zeocin™ (ZeoR)
蛋白标记
His 标签 (6x)、c-Myc 抗原决定簇标签, c-Myc Epitope Tag
克隆方法
限制性内切酶/MCS
载体
pPIC
内容与储存
各 20 µg pPICZ A, B, & C,混悬于 10 mM Tris-HCl、1 mM EDTA(pH 值 8.0)中。还包括 GS115/pPICZ/lacZ 阳性对照菌株。