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Invitrogen
Phospho-ELK1 (Ser383) Monoclonal Antibody (S.110.6)
高级验证
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
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Phospho-ELK1 (Ser383) Antibody (MA5-15225) in WB
Western blot was performed using Anti-Phospho-ELK1 (Ser383) Monoclonal Antibody (S.110.6) (Product # MA5-15225) and a 50 kDa band corresponding to phospho ELK1 (Ser383) was observed upon EGF and Wortmanin treatment. Whole cell extracts (30 µg lysate) of T-47D (Lane 1), T-47D serum starved for 24 Hours (Lane 2), serum starved T-47D treated with EGF (1nM for 30 mins) (Lane 3) and serum starved T-47D treated w... View More
产品信息
MA5-15225
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
S.110.6
抗原
Synthetic phosphopeptide corresponding to residues around pSer383 of human Elk-1
偶联物
形式
Liquid
浓度
905 µg/mL
纯化类型
Affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 50% glycerol, 100µg/mL BSA, 0.15M NaCl
内含物
<0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_10980435
产品详细信息
It is not recommended to aliquot this antibody.
靶标信息
ELK1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. ELK1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by ELK1. ELK1 appears to be a direct target of activated MAP kinase. Biochemical studies indicate that ELK1 is a good substrate for MAP kinase, the kinetics of ELK1 phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block ELK1 activation in vivo. ELK1 is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for ELK1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ELK 1; ELK1, member of ETS oncogene family; ETS domain-containing protein Elk-1; ETS-like gene 1; Oncogene Elk1; tyrosine kinase (ELK1) oncogene
基因别名: ELK1
UniProt ID: (Human) P19419
Entrez Gene ID: (Human) 2002
RNA polymerase II core promoter proximal region sequence-specific DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
winged helix/forkhead transcription factor
transcription from RNA polymerase II promoter
response to light stimulus
cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cellular response to testosterone stimulus
cellular response to gamma radiation
response to fibroblast growth factor
positive regulation of neuron death
