Invitrogen

RAC1 Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

8个已发表图片

23 篇参考文献

查看其他8个RAC1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

RAC1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 20

RAC1 Antibody (PA1-091) in ICC/IF

Immunofluorescence analysis of RAC1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RAC1 Polyclonal Antibody (Product # PA1-091) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790),... View More

RAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAC1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

RAC1 Antibody — Figure 1 Rac1 is overexpressed in breast cancer cells. (a) Upper panel: normal mammary epithelial cells (76N) and breast cancer cells of subtype luminal A (MCF-7 and T47D), and luminal B (BT-474 and ZR-75-1), triple-negative (MDA-MB-231 and MDA-MB-468) and HER2 positive (SkBr3, HCC1954, 21MT-1) were analyzed for Rac1 protein expression by Western blot analysis. As a protein loading control, the level of GAPDH in cell lysates was assessed. Lower panel: immunoblot densities of Rac1 and GAPDH were quantified using ImageJ analytical program (NIH) and relative Rac1 expression versus GAPDH determined. (b) Representative IHC analysis shows a distinct increase in immunostaining of Rac1 in malignant breast tumor tissues compared to normal breast tissues. (c) Box plot shows composite score of Rac1 expression in normal breast/benign tumor tissues (NT) and cancerous breast tissue (Cancer), analyzed by IHC. {TM}

产品信息

PA1-091

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看16篇已发表文章 16篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20-1:50

免疫细胞化学 (ICC/IF)

1:20-1:100

查看4篇已发表文章 4篇已发表文章

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Full-length Human Protein

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2539856

靶标信息

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Proteomic Profiling of Chemotherapy Responses in FOLFOX-Resistant Colorectal Cancer Cells.

International journal of molecular sciences

Tam SY,Islam Khan MZ,Chen JY,Yip JH,Yan HY,Tam TY,Law HK

PA1-091 was used in Western Blotting to compare the difference of chemotherapy responses between FOLFOX-resistant and wild-type colorectal cancer cells by proteomic profiling to suggest novel treatment targets.

Thu Jun 08 00:00:00 EDT 2023

Regulation of serine palmitoyl-transferase and Rac1-Nox2 signaling in diabetic retinopathy.

Scientific reports

Alka K,Mohammad G,Kowluru RA

Published figure using RAC1 polyclonal antibody (Product # PA1-091) in Immunocytochemistry

Thu Oct 06 00:00:00 EDT 2022

Neutrophils interact with cholangiocytes to cause cholestatic changes in alcoholic hepatitis.

Gut

Takeuchi M,Vidigal PT,Guerra MT,Hundt MA,Robert ME,Olave-Martinez M,Aoki S,Khamphaya T,Kersten R,Kruglov E,de la Rosa Rodriguez R,Banales JM,Nathanson MH,Weerachayaphorn J

PA1-091 was used in Western Blotting to examine whether bile ducts participate in the pathogenesis of Alcoholic hepatitis.

Mon Feb 01 00:00:00 EST 2021

RAC1 plays an essential role in estrogen receptor alpha function in breast cancer cells.

Oncogene

Sun J,Gaidosh G,Xu Y,Mookhtiar A,Man N,Cingaram PR,Blumenthal E,Shiekhattar R,Goka ET,Nimer SD,Lippman ME

Published figure using RAC1 polyclonal antibody (Product # PA1-091) in Western Blot

Fri Oct 01 00:00:00 EDT 2021

RAC1 plays an essential role in estrogen receptor alpha function in breast cancer cells.

Oncogene

Sun J,Gaidosh G,Xu Y,Mookhtiar A,Man N,Cingaram PR,Blumenthal E,Shiekhattar R,Goka ET,Nimer SD,Lippman ME

Published figure using RAC1 polyclonal antibody (Product # PA1-091) in Western Blot

Fri Oct 01 00:00:00 EDT 2021

Periostin/Filamin-A: A Candidate Central Regulatory Axis for Valve Fibrogenesis and Matrix Compaction.

Frontiers in cell and developmental biology

Misra S,Ghatak S,Moreno-Rodriguez RA,Norris RA,Hascall VC,Markwald RR

PA1-091 was used in Western Blot to examine whether filamin A has a cross-talk with periostin/signaling that promotes remodeling of postnatal heart valves into mature leaflets.

Thu Feb 09 00:00:00 EST 2023

Periostin/Filamin-A: A Candidate Central Regulatory Axis for Valve Fibrogenesis and Matrix Compaction.

Frontiers in cell and developmental biology

Misra S,Ghatak S,Moreno-Rodriguez RA,Norris RA,Hascall VC,Markwald RR

PA1-091 was used in Western Blot to examine whether filamin A has a cross-talk with periostin/signaling that promotes remodeling of postnatal heart valves into mature leaflets.

Thu Feb 09 00:00:00 EST 2023

Endoplasmic Reticulum Stress Induces CAP2 Expression Promoting Epithelial-Mesenchymal Transition in Liver Cancer Cells.

Molecules and cells

Yoon S,Shin B,Woo HG

PA1-091 was used in Western Blot to suggest that ER stress induces CAP2 expression promoting EMT in liver cancers cells.

Tue Aug 31 00:00:00 EDT 2021

NecroX-5 Can Suppress Melanoma Metastasis by Reducing the Expression of Rho-Family GTPases.

Journal of clinical medicine

Moon GT,Lee JH,Jeong SH,Jin SW,Park YM

PA1-091 was used in Western Blotting to demonstrate that NX-5 can suppress melanoma metastasis by reducing the expression of Rho-family GTPases.

Fri Jun 25 00:00:00 EDT 2021

NecroX-5 Can Suppress Melanoma Metastasis by Reducing the Expression of Rho-Family GTPases.

Journal of clinical medicine

Moon GT,Lee JH,Jeong SH,Jin SW,Park YM

PA1-091 was used in Western Blotting to demonstrate that NX-5 can suppress melanoma metastasis by reducing the expression of Rho-family GTPases.

Fri Jun 25 00:00:00 EDT 2021

EMT-Induced Cell-Mechanical Changes Enhance Mitotic Rounding Strength.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Hosseini K,Taubenberger A,Werner C,Fischer-Friedrich E

Published figure using RAC1 polyclonal antibody (Product # PA1-091) in Western Blot

Thu Oct 01 00:00:00 EDT 2020

Adaptor Protein p66Shc: A Link Between Cytosolic and Mitochondrial Dysfunction in the Development of Diabetic Retinopathy.

Antioxidants & redox signaling

Mishra M,Duraisamy AJ,Bhattacharjee S,Kowluru RA

PA1-091 was used in Immunoprecipitation to investigate the role of p66Shc in the development of diabetic retinopathy.

Wed May 01 00:00:00 EDT 2019

Functional Regulation of an Oxidative Stress Mediator, Rac1, in Diabetic Retinopathy.

Molecular neurobiology

Mohammad G,Duraisamy AJ,Kowluru A,Kowluru RA

PA1-091 was used in Immunocytochemistry-immunoflourescence to investigate the mechanisms of Rac1 activation in the development of diabetic retinopathy.

Sun Dec 01 00:00:00 EST 2019

Activation of endothelial ras-related C3 botulinum toxin substrate 1 (Rac1) improves post-stroke recovery and angiogenesis via activating Pak1 in mice.

Experimental neurology

Bu F,Min JW,Munshi Y,Lai YJ,Qi L,Urayama A,McCullough LD,Li J

PA1-091 was used in Immunohistochemistry-immunofluorescence to examine the functional role of endothelial Ras-related C3 botulinum toxin substrate 1(Rac1) in post-stroke recovery and angiogenesis.

Sun Dec 01 00:00:00 EST 2019

Comparative analysis of viral entry for Asian and African lineages of Zika virus.

Virology

Rinkenberger N,Schoggins JW

PA1-091 was used in Western Blotting to contribute new insight into the ZIKV entry process and suggest that divergent ZIKV strains enter cells in a highly conserved manner.

Mon Jul 01 00:00:00 EDT 2019

Cannabinoid receptor 2 agonist attenuates blood‑brain barrier damage in a rat model of intracerebral hemorrhage by activating the Rac1 pathway.

International journal of molecular medicine

Wang Z,Li Y,Cai S,Li R,Cao G

PA1-091 was used in Western Blotting to study the effect of cannabinoid receptor 2 agonist (CNR2) JWH133 on blood brain barrier integrity and its underlying mechanism.

Thu Nov 01 00:00:00 EDT 2018

AKT-mediated stabilization of histone methyltransferase WHSC1 promotes prostate cancer metastasis.

The Journal of clinical investigation

Li N,Xue W,Yuan H,Dong B,Ding Y,Liu Y,Jiang M,Kan S,Sun T,Ren J,Pan Q,Li X,Zhang P,Hu G,Wang Y,Wang X,Li Q,Qin J

PA1091 was used in immunohistochemistry to demonstrate that WHSC1 drives indolent PTEN-null tumors to become metastatic prostate cancer

Mon Apr 03 00:00:00 EDT 2017

AKT-mediated stabilization of histone methyltransferase WHSC1 promotes prostate cancer metastasis.

The Journal of clinical investigation

Li N,Xue W,Yuan H,Dong B,Ding Y,Liu Y,Jiang M,Kan S,Sun T,Ren J,Pan Q,Li X,Zhang P,Hu G,Wang Y,Wang X,Li Q,Qin J

PA1091 was used in immunohistochemistry to demonstrate that WHSC1 drives indolent PTEN-null tumors to become metastatic prostate cancer

Mon Apr 03 00:00:00 EDT 2017

Di-n-butyl phthalate exposure negatively influences structural and functional neuroplasticity via Rho-GTPase signaling pathways.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Ding Y,Lu L,Xuan C,Han J,Ye S,Cao T,Chen W,Li A,Zhang X

PA1091 was used in western blot to develop and examine a mouse model of chronic di-n-butyl phthalate exposure

Sat Jul 01 00:00:00 EDT 2017

RHOG-DOCK1-RAC1 Signaling Axis Is Perturbed in DHEA-Induced Polycystic Ovary in Rat Model.

Reproductive sciences (Thousand Oaks, Calif.)

Ubba V,Soni UK,Chadchan S,Maurya VK,Kumar V,Maurya R,Chaturvedi H,Singh R,Dwivedi A,Jha RK

PA1091 was used in flow cytometry and western blot to determine the expression and localization of RHOG in normal and polycystic rat ovaries

Mon May 01 00:00:00 EDT 2017

C9ORF72 interaction with cofilin modulates actin dynamics in motor neurons.

Nature neuroscience

Sivadasan R,Hornburg D,Drepper C,Frank N,Jablonka S,Hansel A,Lojewski X,Sterneckert J,Hermann A,Shaw PJ,Ince PG,Mann M,Meissner F,Sendtner M

PA1-091X was used in western blot to elucidate the mechanism by which intronic hexanucleotide expansions in C9ORF72 contribute to amyotrophic lateral sclerosis

Thu Dec 01 00:00:00 EST 2016

RAC1 GTPase promotes the survival of breast cancer cells in response to hyper-fractionated radiation treatment.

Oncogene

Hein AL,Post CM,Sheinin YM,Lakshmanan I,Natarajan A,Enke CA,Batra SK,Ouellette MM,Yan Y

PA1-091 was used in western blot to study promotion of survival of breast cancer cells in response to hyper-fractionated radiation treatment by RAC1 GTPase

Thu Dec 08 00:00:00 EST 2016

MicroRNA-27a Inhibits Cell Migration and Invasion of Fibroblast-Like Synoviocytes by Targeting Follistatin-Like Protein 1 in Rheumatoid Arthritis.

Molecules and cells

Shi DL,Shi GR,Xie J,Du XZ,Yang H

PA1-091 was used in western blot to investigate the effects of miR-27a on cell migration and invasion in fibroblast-like synoviocytes from rheumatoid arthritis patients