Invitrogen

STAT3 Monoclonal Antibody (9D8)

高级验证

IP-mass spectrometry

12个已发表图片

22 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

STAT3 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 28

STAT3 Antibody (MA1-13042) in ICC/IF

Immunofluorescence analysis of Signal transducer and activator of transcription 3 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 5 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with STAT3 Monoclonal Antibody (9D8) (Product # MA1-13042) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Seconda... View More

STAT3 Antibody in Immunohistochemistry (IHC)

STAT3 Antibody in Immunoprecipitation (IP)

STAT3 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Product Image Gallery

STAT3 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of STAT3 was observed in HeLa, Hep G2, SK-N-MC, HaCaT and Jurkat but not in PC-3 using STAT3 Monoclonal Antibody (9D8) (Product # MA1-13042) in western blot. {RE}

产品信息

MA1-13042

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5000-1:10,000

查看16篇已发表文章 16篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:1,600

免疫细胞化学 (ICC/IF)

1:100

查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

2 µg

染色质免疫沉淀实验 (ChIP)

1-3 µL

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

9D8

抗原

Residues 655-770 from human recombinant protein expressed in bacteria

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10985240

产品详细信息

MA1-13042 detects STAT3 from human, mouse, monkey and rat samples.

MA1-13042 has been successfully used in Western blot, ChIP, immunofluorescence, immunohistochemistry (paraffin), and immunoprecipitation procedures. By Western blot, this antibody detects a 88 kDa protein
corresponding to STAT3. Positive control: HepG2 cells.

The MA1-13042 antigen is a recombinant protein corresponding to residues 655-770 of human STAT3.

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

靶标信息

STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Remifentanil modulates the TLR4‑mediated MMP‑9/TIMP1 balance and NF‑κB/STAT3 signaling in LPS‑induced A549 cells.

Experimental and therapeutic medicine

Chang J,Zhang W

MA1-13042 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show that remifentanil downregulated TLR4 to reduce MMP-9/TIMP1 imbalance to inhibit inflammatory dysfunction in LPS-treated A549 cells, by regulating NF-κB/STAT3 signaling.

Wed Feb 01 00:00:00 EST 2023

Diospyros lotus leaf extract and its main component myricitrin regulate pruritus through the inhibition of astrocyte activation.

Experimental and therapeutic medicine

Shin JY,Cho BO,Park JH,Kang ES,Kim YS,Jang SI

MA1-13042 was used in Western Blotting to suggest that Diospyros lotus leaf extract and myricetin can be used as oral therapeutic agents for the treatment and management of pruritus.

Sat Jul 01 00:00:00 EDT 2023

Blocking STAT3/5 through direct or upstream kinase targeting in leukemic cutaneous T-cell lymphoma.

EMBO molecular medicine

Sorger H,Dey S,Vieyra-Garcia PA,Pölöske D,Teufelberger AR,de Araujo ED,Sedighi A,Graf R,Spiegl B,Lazzeri I,Braun T,Garces de Los Fayos Alonso I,Schlederer M,Timelthaler G,Kodajova P,Pirker C,Surbek M,Machtinger M,Graier T,Perchthaler I,Pan Y,Fink-Puches R,Cerroni L,Ober J,Otte M,Albrecht JD,Tin G,Abdeldayem A,Manaswiyoungkul P,Olaoye OO,Metzelder ML,Orlova A,Berger W,Wobser M,Nicolay JP,André F,Nguyen VA,Neubauer HA,Fleck R,Merkel O,Herling M,Heitzer E,Gunning PT,Kenner L,Moriggl R,Wolf P

Published figure using STAT3 monoclonal antibody (Product # MA1-13042) in Western Blot

Wed Dec 07 00:00:00 EST 2022

Abi1 mediates airway smooth muscle cell proliferation and airway remodeling via Jak2/STAT3 signaling.

iScience

Wang R,Wang Y,Liao G,Chen B,Panettieri RA,Penn RB,Tang DD

MA1-13042 was used in Immunocytochemistry to identify Abi1 as a molecular switch that activates Jak2 kinase and STAT3 in ASM cells and demonstrate that a dysfunctional Abi1-associated pathway contributes to the progression of asthma.

Fri Feb 18 00:00:00 EST 2022

Abi1 mediates airway smooth muscle cell proliferation and airway remodeling via Jak2/STAT3 signaling.

iScience

Wang R,Wang Y,Liao G,Chen B,Panettieri RA,Penn RB,Tang DD

Fri Feb 18 00:00:00 EST 2022

Abi1 mediates airway smooth muscle cell proliferation and airway remodeling via Jak2/STAT3 signaling.

iScience

Wang R,Wang Y,Liao G,Chen B,Panettieri RA,Penn RB,Tang DD

Fri Feb 18 00:00:00 EST 2022

Prevention of Testicular Damage by Indole Derivative MMINA via Upregulated StAR and CatSper Channels with Coincident Suppression of Oxidative Stress and Inflammation: In Silico and In Vivo Validation.

Antioxidants (Basel, Switzerland)

Afsar T,Razak S,Trembley JH,Khan K,Shabbir M,Almajwal A,Alruwaili NW,Ijaz MU

MA1-13042 was used in Western Blotting to reveal that MMINA boosted the antioxidant defense mechanism, restored the levels of fertility hormones, and suppressed histomorphological alterations.

Wed Oct 19 00:00:00 EDT 2022

Antioxidants (Basel, Switzerland)

Afsar T,Razak S,Trembley JH,Khan K,Shabbir M,Almajwal A,Alruwaili NW,Ijaz MU

MA1-13042 was used in Western Blotting to reveal that MMINA boosted the antioxidant defense mechanism, restored the levels of fertility hormones, and suppressed histomorphological alterations.

Wed Oct 19 00:00:00 EDT 2022

Role of Oncostatin M in the Pathogenesis of Vernal Keratoconjunctivitis: Focus on the Barrier Function of the Epithelium and Interleukin-33 Production by Fibroblasts.

Investigative ophthalmology & visual science

Ninomiya I,Yamatoya K,Mashimo K,Matsuda A,Usui-Ouchi A,Araki Y,Ebihara N

Published figure using STAT3 monoclonal antibody (Product # MA1-13042) in Western Blot

Thu Dec 01 00:00:00 EST 2022

Penta-O-Galloyl-β-D-Glucose in Pistacia integerrima Targets AMPK-ULK1 and ERK/STAT3 Signaling Axes to Induce ROS-Independent Autophagic Cell Death in Human Lung Cancer Cells.

Frontiers in pharmacology

Balkrishna A,Mulay VP,Verma S,Srivastava J,Lochab S,Varshney A

MA1-13042 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to propose PGG as a potential AMPK activator and STAT3 inhibitor that can be exploited further in developing adjuvant chemotherapeutics against lung cancer.

Sat Aug 06 00:00:00 EDT 2022

Novel TPR::ROS1 Fusion Gene Activates MAPK, PI3K and JAK/STAT Signaling in an Infant-type Pediatric Glioma.

Cancer genomics & proteomics

Deland L,Keane S,Bontell TO,Fagman H,Sjögren H,Lind AE,Carén H,Tisell M,Nilsson JA,Ejeskär K,Sabel M,Abel F

MA1-13042 was used in Western Blotting to identify and characterize a novel TPR::ROS1 fusion gene in an infant-type hemispheric glioma, shedding light on the activated oncogenic pathways and potential targeted therapies.

Thu Nov 03 00:00:00 EDT 2022

Myelin repair is fostered by the corticosteroid medrysone specifically acting on astroglial subpopulations.

EBioMedicine

Silva Oliveira Junior M,Schira-Heinen J,Reiche L,Han S,de Amorim VCM,Lewen I,Gruchot J,Göttle P,Akkermann R,Azim K,Küry P

MA1-13042 was used in Immunohistochemistry to analyse demyelination and remyelination related effects on astrocytes and stem cell niches and study the consequences of medrysone application on myelin repair, and astrocyte polarization.

Thu Sep 01 00:00:00 EDT 2022

miRNAs Potentially Involved in Post Lung Transplant-Obliterative Bronchiolitis: The Role of miR-21-5p.

Cells

Bozzini S,Pandolfi L,Rossi E,Inghilleri S,Zorzetto M,Ferrario G,Di Carlo S,Politano G,De Silvestri A,Frangipane V,Porzio M,Kessler R,Calabrese F,Meloni F,Morbini P

Published figure using STAT3 monoclonal antibody (Product # MA1-13042) in Western Blot

Sat Mar 20 00:00:00 EDT 2021

Discovery of a novel potentially transforming somatic mutation in CSF2RB gene in breast cancer.

Cancer medicine

Rashid M,Ali R,Almuzzaini B,Song H,AlHallaj A,Abdulkarim AA,Mohamed Baz O,Al Zahrani H,Mustafa Sabeena M,Alharbi W,Hussein M,Boudjelal M

MA1-13042 was used in Western Blotting to report the discovery of a mutation in the CSF2RB gene of a breast cancer patient and the cell line KAIMRC1.

Mon Nov 01 00:00:00 EDT 2021

Discovery of a novel potentially transforming somatic mutation in CSF2RB gene in breast cancer.

Cancer medicine

Rashid M,Ali R,Almuzzaini B,Song H,AlHallaj A,Abdulkarim AA,Mohamed Baz O,Al Zahrani H,Mustafa Sabeena M,Alharbi W,Hussein M,Boudjelal M

MA1-13042 was used in Western Blotting to report the discovery of a mutation in the CSF2RB gene of a breast cancer patient and the cell line KAIMRC1.

Mon Nov 01 00:00:00 EDT 2021

Inhibition of IL-6 in the LCWE Mouse Model of Kawasaki Disease Inhibits Acute Phase Reactant Serum Amyloid A but Fails to Attenuate Vasculitis.

Frontiers in immunology

Porritt RA,Chase Huizar C,Dick EJ,Kumar S,Escalona R,Gomez AC,Marek-Iannucci S,Noval Rivas M,Patterson J,Forsthuber TG,Arditi M,Gorelik M

MA1-13042 was used in Immunohistochemistry to show that both STAT3 and IL-6 were dispensable for disease development indicating they may be bystanders of inflammation.

Mon Sep 20 00:00:00 EDT 2021

Inhibition of IL-6 in the LCWE Mouse Model of Kawasaki Disease Inhibits Acute Phase Reactant Serum Amyloid A but Fails to Attenuate Vasculitis.

Frontiers in immunology

Porritt RA,Chase Huizar C,Dick EJ,Kumar S,Escalona R,Gomez AC,Marek-Iannucci S,Noval Rivas M,Patterson J,Forsthuber TG,Arditi M,Gorelik M

MA1-13042 was used in Immunohistochemistry to show that both STAT3 and IL-6 were dispensable for disease development indicating they may be bystanders of inflammation.

Mon Sep 20 00:00:00 EDT 2021

The CDK4/6-EZH2 pathway is a potential therapeutic target for psoriasis.

The Journal of clinical investigation

Müller A,Dickmanns A,Resch C,Schäkel K,Hailfinger S,Dobbelstein M,Schulze-Osthoff K,Kramer D

MA1-13042 was used in Chromatin immunoprecipitation to find a hyperactivation of the CDK4/6-EZH2 pathway in human and mouse psoriatic skin lesions.

Mon Nov 02 00:00:00 EST 2020

MicroRNA-325-3p Facilitates Immune Escape of Mycobacterium tuberculosis through Targeting LNX1 via NEK6 Accumulation to Promote Anti-Apoptotic STAT3 Signaling.

mBio

Fu B,Xue W,Zhang H,Zhang R,Feldman K,Zhao Q,Zhang S,Shi L,Pavani KC,Nian W,Lin X,Wu H

MA1-13042 was used in Western Blotting to contribution to the understanding of the immune escape of M. tuberculosis, and they provide a theoretical basis for the development of therapeutic approaches for drug-resistant TB.

Tue Jun 02 00:00:00 EDT 2020

MicroRNA-325-3p Facilitates Immune Escape of Mycobacterium tuberculosis through Targeting LNX1 via NEK6 Accumulation to Promote Anti-Apoptotic STAT3 Signaling.

mBio

Fu B,Xue W,Zhang H,Zhang R,Feldman K,Zhao Q,Zhang S,Shi L,Pavani KC,Nian W,Lin X,Wu H

Tue Jun 02 00:00:00 EDT 2020

Periostin Activation of Integrin Receptors on Sensory Neurons Induces Allergic Itch.

Cell reports

Mishra SK,Wheeler JJ,Pitake S,Ding H,Jiang C,Fukuyama T,Paps JS,Ralph P,Coyne J,Parkington M,DeBrecht J,Ehrhardt-Humbert LC,Cruse GP,Bäumer W,Ji RR,Ko MC,Olivry T

MA1-13042 was used in Western Blotting to show that periostin, abundantly expressed in the skin of patients with atopic dermatitis (AD), induces itch in mice, dogs, and monkeys.

Tue Apr 07 00:00:00 EDT 2020

Fatty acids and cancer-amplified ZDHHC19 promote STAT3 activation through S-palmitoylation.

Nature

Niu J,Sun Y,Chen B,Zheng B,Jarugumilli GK,Walker SR,Hata AN,Mino-Kenudson M,Frank DA,Wu X

Published figure using STAT3 monoclonal antibody (Product # MA1-13042) in Western Blot

Sun Sep 01 00:00:00 EDT 2019

Fatty acids and cancer-amplified ZDHHC19 promote STAT3 activation through S-palmitoylation.

Nature

Niu J,Sun Y,Chen B,Zheng B,Jarugumilli GK,Walker SR,Hata AN,Mino-Kenudson M,Frank DA,Wu X

Published figure using STAT3 monoclonal antibody (Product # MA1-13042) in Western Blot

Sun Sep 01 00:00:00 EDT 2019

Effects of cell-cell crosstalk on gene expression patterns in a cell model of renal cell carcinoma lung metastasis.

International journal of oncology

Kaminska K,Czarnecka AM,Khan MI,Fendler W,Klemba A,Krasowski P,Bartnik E,Szczylik C

MA1-13042 was used in Western Blotting to study the contribution of cell-cell communication in the development of the metastatic niche in renal cell carcinoma.

Thu Mar 01 00:00:00 EST 2018

Whole-genome sequencing reveals oncogenic mutations in mycosis fungoides.

Blood

McGirt LY,Jia P,Baerenwald DA,Duszynski RJ,Dahlman KB,Zic JA,Zwerner JP,Hucks D,Dave U,Zhao Z,Eischen CM

MA1-13042 was used in western blot to identify factors that contribute to mycosis fungoides pathogenesis

Thu Jul 23 00:00:00 EDT 2015

An Autocrine Cytokine/JAK/STAT-Signaling Induces Kynurenine Synthesis in Multidrug Resistant Human Cancer Cells.

PloS one

Campia I,Buondonno I,Castella B,Rolando B,Kopecka J,Gazzano E,Ghigo D,Riganti C

MA1-13042 was used in western blot to analyze multidrug resistant human cancer cells and autocrime cytokine/JAK/STAT-signaling induces kynurenin synthesis

Tue Feb 02 00:00:00 EST 2016

FGF2 regulates melanocytes viability through the STAT3-transactivated PAX3 transcription.

Cell death and differentiation

Dong L,Li Y,Cao J,Liu F,Pier E,Chen J,Xu Z,Chen C,Wang RA,Cui R

MA1-13042 was used in western blot to identify the regulation and function of the paired box 3 gene.

Sun Apr 01 00:00:00 EDT 2012

生物信息学

蛋白别名: acute phase response factor; Acute-phase response factor; DNA-binding protein APRF; FLJ20882; MGC16063; Signal transducer and activator of transcription 3; signal transducer and activator of transcription 3 (acute-phase response factor); STAT

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基因别名: 1110034C02Rik; ADMIO; ADMIO1; APRF; AW109958; HIES; STAT3

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UniProt ID: (Human) P40763, (Rat) P52631, (Mouse) P42227

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Entrez Gene ID: (Human) 6774, (Rat) 25125, (Mouse) 20848

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功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II repressing transcription factor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding signal transducer activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding transcription factor binding protein kinase binding protein phosphatase binding chromatin DNA binding CCR5 chemokine receptor binding glucocorticoid receptor binding identical protein binding transcription regulatory region DNA binding protein dimerization activity RNA polymerase II transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding DNA-binding transcription factor

参与通路

STAT3 Monoclonal Antibody (9D8)

STAT3 Antibody (MA1-13042) in ICC/IF

产品信息

MA1-13042

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

STAT3 Monoclonal Antibody (9D8)

STAT3 Antibody (MA1-13042) in ICC/IF

产品信息

MA1-13042

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)