Invitrogen

c-Fos Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 篇参考文献

查看其他44个c-Fos抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (3)
高级验证 (1)

c-Fos Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 4

c-Fos Antibody (PA1-830) in ICC/IF

Immunofluorescent analysis of c-FOS (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing c-FOS (Product # PA1-830), at a dilution of 1:500 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody at a dilution of ... View More

Product Image Gallery

c-Fos Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot of c-Fos using c-Fos Polyclonal Antibody (Product # PA1-830), shows expression of 40KDa isoform of c-Fos in HeLa cell line upon TPA treatment. {TM}

产品信息

PA1-830

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:500

产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues S(4) G F N A D Y E A S S S R C(17) of human cFos.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

PBS

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2106777

产品详细信息

PA1-830 detects cFos from mouse and human cells.

PA1-830 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~60 kDa protein representing cFos in NIH 3T3 serum stimulated cells.

The PA1-830 immunogen is a synthetic peptide corresponding to residues S(4) G F N A D Y E A S S S R C(17) of human cFos. This sequence is completely conserved in mouse cFos.

Reconstitute with 200 µL of PBS (1 mg/mL).

靶标信息

FBJ murine osteosarcoma viral oncogene homolog is commonly known as Cellular protooncogene Fos (c-Fos). It belongs to the fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14. c-Fos heterodimerizes with c-Jun to form the Activator Protein -1 (AP-1) transcription factor which regulates transcription of several genes that play an important role in cellular signal transduction, cell proliferation and differentiation. c-Fos has been shown to be phosphorylated by various stimuli including growth factors and insulin at atleast seven different sites. The best known upstream kinase for phosphorylation at Threonine 232 is ERK MAPK which regulates localization of c-Fos to the nucleus and is thus important for c-Fos induced transcriptional activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Optogenetics stimulates nerve reorganization in the contralesional anterolateral primary motor cortex in a mouse model of ischemic stroke.

Neural regeneration research

Gao BY,Cao YX,Fu PF,Xing Y,Liang D,Jiang S,Xie YX,Li M

PA1-830 was used in Immunocytochemistry, Western Blot, Immunohistochemistry to suggest that optogenetic cALM stimulation promotes neural reorganization in the primary motor cortex of the ischemic hemisphere, and that optogenetic cALM inhibition and activation have different effects on neural plasticity.

Fri Jul 01 00:00:00 EDT 2022

25-Hydroxycholesterol acts as an amplifier of inflammatory signaling.

Proceedings of the National Academy of Sciences of the United States of America

Gold ES,Diercks AH,Podolsky I,Podyminogin RL,Askovich PS,Treuting PM,Aderem A

PA1-830 was used in ChIP assay and western blot to investigate the role of oxysterol 25-hydroxycholesterol during infection with influenza

Tue Jul 22 00:00:00 EDT 2014

生物信息学

蛋白别名: activator protein 1; cellular oncogene c-fos; Cellular oncogene fos; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS); FBJ murine osteosarcoma viral oncogene homolog; FBJ osteosarcoma oncogene; Fos proto-oncogene, AP-1 trancription factor subunit; Fos proto-oncogene, AP-1 transcription factor subunit; G0/G1 switch regulatory protein 7; Protein c-Fos; Proto-oncogene c-Fos; proto-oncogene protein c-fos; Transcription factor AP-1 subunit c-Fos

查看更多收起

基因别名: AP-1; C-FOS; cFos; D12Rfj1; FOS; G0S7; p55

查看更多收起

UniProt ID: (Human) P01100, (Mouse) P01101

查看更多收起

Entrez Gene ID: (Human) 2353, (Mouse) 14281

查看更多收起

功能