Invitrogen

mTOR Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

19个已发表图片

20 篇参考文献

查看其他46个mTOR抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

mTOR Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 33

mTOR Antibody (PA5-34663) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of mTOR was performed in MCF-7 cells fixed in ice-cold MeOH for 5 min. Green: mTOR Polyclonal Antibody (Product # PA5-34663) diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

mTOR Antibody in Immunohistochemistry (Paraffin) (IHC (P))

mTOR Antibody in Immunoprecipitation (IP)

mTOR Antibody in Immunohistochemistry (IHC)

mTOR Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

mTOR Antibody — Antibody specificity was demonstrated by shRNA mediated knockdown of the target protein. 293T cells were transfected with mTOR shRNA and decrease in signal intensity was observed in western blot application using anti mTOR Polyclonal Antibody (Product # PA5-34663). {KD}

产品信息

PA5-34663

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看11篇已发表文章 11篇已发表文章

免疫组化 (IHC)

查看4篇已发表文章 4篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

1:100-1:500

查看1篇已发表文章 1篇已发表文章

染色质免疫沉淀实验 (ChIP)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 2386 and 2549 of mTOR (Uniprot ID#P42345)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.05 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2552015

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain, Flag-human mTOR transfected 293T.

Predicted reactivity: Mouse (99%), Rat (100%), Zebrafish (90%), Sheep (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with numerous forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are currently used as immunosuppressants and cancer therapeutics. Mutations affecting the gene results in Smith-Kingsmore syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Silibinin Downregulates Types I and III Collagen Expression via Suppression of the mTOR Signaling Pathway.

International journal of molecular sciences

Choi S,Ham S,Lee YI,Kim J,Lee WJ,Lee JH

PA5-34663 was used in Western Blot to suggest the potential of silibinin as a promising therapeutic agent for preventing and treating keloid scars.

Thu Sep 21 00:00:00 EDT 2023

Three-dimensional growth reveals fine-tuning of 5-lipoxygenase by proliferative pathways in cancer.

Life science alliance

Göbel T,Goebel B,Hyprath M,Lamminger I,Weisser H,Angioni C,Mathes M,Thomas D,Kahnt AS

PA5-34663 was used in Western Blotting to show that tumor cells fine-tune 5-LO and LT biosynthesis in response to environmental changes repressing the enzyme during proliferation while making use of the enzyme under cell stress conditions, implying that tumor-derived 5-LO plays a role in the manipulation of the tumor stroma to quickly restore cell proliferation.

Mon May 01 00:00:00 EDT 2023

Immunohistochemical Expression Pattern of FGFR1, FGFR2, RIP5, and HIP2 in Developing and Postnatal Kidneys of (yotari) Mice.

International journal of molecular sciences

Kelam N,Racetin A,Katsuyama Y,Vukojević K,Kostić S

Published figure using mTOR polyclonal antibody (Product # PA5-34663) in Immunohistochemistry

Fri Feb 11 00:00:00 EST 2022

Immunohistochemical Expression Pattern of FGFR1, FGFR2, RIP5, and HIP2 in Developing and Postnatal Kidneys of (yotari) Mice.

International journal of molecular sciences

Kelam N,Racetin A,Katsuyama Y,Vukojević K,Kostić S

Published figure using mTOR polyclonal antibody (Product # PA5-34663) in Immunohistochemistry

Fri Feb 11 00:00:00 EST 2022

LINC00960 regulates cell proliferation and glycolysis in pancreatic cancer through the miR-326-3p/TUFT1/AKT-mTOR axis.

The Kaohsiung journal of medical sciences

Zhou B,Lei JH,Wang Q,Qu TF,Cha LC,Zhan HX,Liu SL,Hu X,Sun CD,Cao JY,Qiu FB,Guo WD

Published figure using mTOR polyclonal antibody (Product # PA5-34663) in Western Blot

Thu Dec 01 00:00:00 EST 2022

Inhibition of the immunoproteasome modulates innate immunity to ameliorate muscle pathology of dysferlin-deficient BlAJ mice.

Cell death & disease

Farini A,Tripodi L,Villa C,Napolitano F,Strati F,Molinaro D,Facciotti F,Cassani B,Torrente Y

Published figure using mTOR polyclonal antibody (Product # PA5-34663) in Western Blot

Sat Nov 19 00:00:00 EST 2022

Diane-35 and Metformin Induce Autophagy and Apoptosis in Polycystic Ovary Syndrome Women with Early-Stage Endometrial Carcinoma.

Genes

Liu Y,Wang Y,Yao D,Chen X,Zhang F,Feng Y,Li X

PA5-34663 was used in Western Blot to support the use of Diane-35 and metformin combination therapy for patients with PCOS and early EC.

Wed Jan 12 00:00:00 EST 2022

Ontogenetic Changes in the Expression of the Lin28 Protein in the Rat Hypothalamic Tuberal Nuclei.

International journal of molecular sciences

Anfimova PA,Pankrasheva LG,Moiseev KY,Shirina ES,Porseva VV,Masliukov PM

PA5-34663 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show for the first time the presence of Lin28-immunoreactive (IR) neurons in the ventromedial nucleus (VMH) and their absence in the dorsomedial and arcuate nuclei in all studied animals.

Thu Nov 03 00:00:00 EDT 2022

ACSL4 contributes to sevoflurane-induced ferroptotic neuronal death in SH-SY5Y cells via the 5' AMP-activated protein kinase/mammalian target of rapamycin pathway.

Annals of translational medicine

Cheng L,Zhu X,Liu Y,Zhu K,Lin K,Li F

Published figure using mTOR polyclonal antibody (Product # PA5-34663) in Western Blot

Wed Sep 01 00:00:00 EDT 2021

ACSL4 contributes to sevoflurane-induced ferroptotic neuronal death in SH-SY5Y cells via the 5' AMP-activated protein kinase/mammalian target of rapamycin pathway.

Annals of translational medicine

Cheng L,Zhu X,Liu Y,Zhu K,Lin K,Li F

Published figure using mTOR polyclonal antibody (Product # PA5-34663) in Western Blot

Wed Sep 01 00:00:00 EDT 2021

Implications of cell division cycle associated 4 on the Wilm's tumor cells viability via AKT/mTOR signaling pathway.

Renal failure

Li S,Qin C,Chen Y,Wei D,Tan Z,Meng J

Published figure using mTOR polyclonal antibody (Product # PA5-34663) in Western Blot

Wed Dec 01 00:00:00 EST 2021

Selective Deletion of the Mechanistic Target of Rapamycin From the Renal Collecting Duct Principal Cell in Mice Down-Regulates the Epithelial Sodium Channel.

Frontiers in physiology

Chen B,Fluitt MB,Brown AL,Scott S,Gadicherla A,Ecelbarger CM

Published figure using mTOR polyclonal antibody (Product # PA5-34663) in Immunohistochemistry

Wed Dec 20 00:00:00 EST 2023

Selective Deletion of the Mechanistic Target of Rapamycin From the Renal Collecting Duct Principal Cell in Mice Down-Regulates the Epithelial Sodium Channel.

Frontiers in physiology

Chen B,Fluitt MB,Brown AL,Scott S,Gadicherla A,Ecelbarger CM

Published figure using mTOR polyclonal antibody (Product # PA5-34663) in Immunohistochemistry

Wed Dec 20 00:00:00 EST 2023

MM-129 as a Novel Inhibitor Targeting PI3K/AKT/mTOR and PD-L1 in Colorectal Cancer.

Cancers

Hermanowicz JM,Pawlak K,Sieklucka B,Czarnomysy R,Kwiatkowska I,Kazberuk A,Surazynski A,Mojzych M,Pawlak D

Published figure using mTOR polyclonal antibody (Product # PA5-34663) in Western Blot

Sat Jun 26 00:00:00 EDT 2021

MM-129 as a Novel Inhibitor Targeting PI3K/AKT/mTOR and PD-L1 in Colorectal Cancer.

Cancers

Hermanowicz JM,Pawlak K,Sieklucka B,Czarnomysy R,Kwiatkowska I,Kazberuk A,Surazynski A,Mojzych M,Pawlak D

Published figure using mTOR polyclonal antibody (Product # PA5-34663) in Western Blot

Sat Jun 26 00:00:00 EDT 2021

Connexin Expression Is Altered in Liver Development of Yotari (dab1 -/-) Mice.

International journal of molecular sciences

Paštar V,Lozić M,Kelam N,Filipović N,Bernard B,Katsuyama Y,Vukojević K

PA5-34663 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to emphasize connexins as a goal-keeper in the maintenance of liver homeostasis and Cxs and Panxs could serve as potential biomarkers and drug targets in liver pathology and diagnostics.

Sat Oct 02 00:00:00 EDT 2021

Actionable Cytopathogenic Host Responses of Human Alveolar Type 2 Cells to SARS-CoV-2.

Molecular cell

Hekman RM,Hume AJ,Goel RK,Abo KM,Huang J,Blum BC,Werder RB,Suder EL,Paul I,Phanse S,Youssef A,Alysandratos KD,Padhorny D,Ojha S,Mora-Martin A,Kretov D,Ash PEA,Verma M,Zhao J,Patten JJ,Villacorta-Martin C,Bolzan D,Perea-Resa C,Bullitt E,Hinds A,Tilston-Lunel A,Varelas X,Farhangmehr S,Braunschweig U,Kwan JH,McComb M,Basu A,Saeed M,Perissi V,Burks EJ,Layne MD,Connor JH,Davey R,Cheng JX,Wolozin BL,Blencowe BJ,Wuchty S,Lyons SM,Kozakov D,Cifuentes D,Blower M,Kotton DN,Wilson AA,Mühlberger E,Emili A

PA5-34663 was used in Functional network inference to perform a quantitative phosphoproteomic survey of induced pluripotent stem cell-derived AT2s infected with SARS-CoV-2 at air-liquid interface, to understand the mechanisms driving infection and pathology.

Thu Dec 17 00:00:00 EST 2020

Interaction of YAP1 and mTOR promotes bladder cancer progression.

International journal of oncology

Xu M,Gu M,Zhou J,Da J,Wang Z

Published figure using mTOR polyclonal antibody (Product # PA5-34663) in Western Blot

Wed Jan 01 00:00:00 EST 2020

Interaction of YAP1 and mTOR promotes bladder cancer progression.

International journal of oncology

Xu M,Gu M,Zhou J,Da J,Wang Z

Published figure using mTOR polyclonal antibody (Product # PA5-34663) in Western Blot

Wed Jan 01 00:00:00 EST 2020

Inhibition of glucose metabolism selectively targets autoreactive follicular helper T cells.

Nature communications

Choi SC,Titov AA,Abboud G,Seay HR,Brusko TM,Roopenian DC,Salek-Ardakani S,Morel L

Published figure using mTOR polyclonal antibody (Product # PA5-34663) in Immunofluorescence

Mon Oct 22 00:00:00 EDT 2018

In situ characterization of the mTORC1 during adipogenesis of human adult stem cells on chip.

Proceedings of the National Academy of Sciences of the United States of America

Wu X,Schneider N,Platen A,Mitra I,Blazek M,Zengerle R,Schüle R,Meier M

Published figure using mTOR polyclonal antibody (Product # PA5-34663) in Western Blot

Tue Jul 19 00:00:00 EDT 2016

In situ characterization of the mTORC1 during adipogenesis of human adult stem cells on chip.

Proceedings of the National Academy of Sciences of the United States of America

Wu X,Schneider N,Platen A,Mitra I,Blazek M,Zengerle R,Schüle R,Meier M

Published figure using mTOR polyclonal antibody (Product # PA5-34663) in Western Blot

Tue Jul 19 00:00:00 EDT 2016

In situ characterization of the mTORC1 during adipogenesis of human adult stem cells on chip.

Proceedings of the National Academy of Sciences of the United States of America

Wu X,Schneider N,Platen A,Mitra I,Blazek M,Zengerle R,Schüle R,Meier M

Published figure using mTOR polyclonal antibody (Product # PA5-34663) in Western Blot

Tue Jul 19 00:00:00 EDT 2016

生物信息学

蛋白别名: angiopoietin-like factor CDT6; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FKBP-rapamycin associated protein (FRAP); FKBP-rapamycin-associated protein FRAP; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; m-TOR; Mammalian target of rapamycin; Mechanistic target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mTOR; mTORC1; Rapamycin and FKBP12 target 1; rapamycin and FKBP12 target-1 protein; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1; Serine/threonine-protein kinase mTOR

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基因别名: 2610315D21Rik; AI327068; flat; FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; SKS

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UniProt ID: (Human) P42345, (Mouse) Q9JLN9, (Rat) P42346

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Entrez Gene ID: (Human) 2475, (Mouse) 56717, (Rat) 56718

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功能

RNA polymerase III type 1 promoter DNA binding RNA polymerase III type 2 promoter DNA binding RNA polymerase III type 3 promoter DNA binding TFIIIC-class transcription factor binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding kinase activity protein kinase binding protein domain specific binding ribosome binding phosphoprotein binding nucleotide binding drug binding transferase activity transferase activity, transferring phosphorus-containing groups phosphotransferase activity, alcohol group as acceptor protein dimerization activity non-receptor serine/threonine protein kinase protein modifying enzyme

参与通路

mTOR Polyclonal Antibody

mTOR Antibody (PA5-34663) in ICC/IF

产品信息

PA5-34663

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

mTOR Polyclonal Antibody

mTOR Antibody (PA5-34663) in ICC/IF

产品信息

PA5-34663

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)