Invitrogen

Pan Ras Monoclonal Antibody (Ras10)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

7个已发表图片

14 篇参考文献

查看其他8个Pan Ras抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Pan Ras Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 23

Pan Ras Antibody (MA1-012) in ICC/IF

Immunofluorescent analysis of Ras (green) in U20S cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing Ras (Product # MA1-012), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Product # 35502) at a ... View More

Pan Ras Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Pan Ras Antibody in Immunoprecipitation (IP)

Pan Ras Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

产品信息

MA1-012

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看11篇已发表文章 11篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:500

免疫细胞化学 (ICC/IF)

1:50-1:200

流式细胞分析 (Flow)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

2 µg

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse, Non-human primate

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

Ras10

抗原

Purified recombinant Ras protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2536664

产品详细信息

This antibody recognizes normal p21, activated p21, and p21 from each of the Harvey, Kirsten and N-ras families.

靶标信息

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

PD-1 blockade delays tumor growth by inhibiting an intrinsic SHP2/Ras/MAPK signalling in thyroid cancer cells.

Journal of experimental & clinical cancer research : CR

Liotti F,Kumar N,Prevete N,Marotta M,Sorriento D,Ieranò C,Ronchi A,Marino FZ,Moretti S,Colella R,Puxeddu E,Paladino S,Kano Y,Ohh M,Scala S,Melillo RM

Published figure using Pan Ras monoclonal antibody (Product # MA1-012) in Immunoprecipitation

Thu Jan 07 00:00:00 EST 2021

PD-1 blockade delays tumor growth by inhibiting an intrinsic SHP2/Ras/MAPK signalling in thyroid cancer cells.

Journal of experimental & clinical cancer research : CR

Liotti F,Kumar N,Prevete N,Marotta M,Sorriento D,Ieranò C,Ronchi A,Marino FZ,Moretti S,Colella R,Puxeddu E,Paladino S,Kano Y,Ohh M,Scala S,Melillo RM

Published figure using Pan Ras monoclonal antibody (Product # MA1-012) in Immunoprecipitation

Thu Jan 07 00:00:00 EST 2021

Evaluating the Epithelial-Mesenchymal Program in Human Breast Epithelial Cells Cultured in Soft Agar Using a Novel Macromolecule Extraction Protocol.

Cancers

Lau HY,Tang J,Casey PJ,Wang M

MA1-012 was used in Western Blotting to investigate the role of epithelial-mesenchymal transition in human mammary epithelial cell colony formation inagar.

Mon Feb 15 00:00:00 EST 2021

Evaluating the Epithelial-Mesenchymal Program in Human Breast Epithelial Cells Cultured in Soft Agar Using a Novel Macromolecule Extraction Protocol.

Cancers

Lau HY,Tang J,Casey PJ,Wang M

MA1-012 was used in Western Blotting to investigate the role of epithelial-mesenchymal transition in human mammary epithelial cell colony formation inagar.

Mon Feb 15 00:00:00 EST 2021

Pharmacokinetic and Pharmacodynamic Factors Contribute to Synergism between Let-7c-5p and 5-Fluorouracil in Inhibiting Hepatocellular Carcinoma Cell Viability.

Drug metabolism and disposition: the biological fate of chemicals

Jilek JL,Tu MJ,Zhang C,Yu AM

MA1-012 was used in Western Blot to identify small-molecule drugs that may synergistically act with the bioengineered microRNA let-7c against hepatocellular carcinoma.

Tue Dec 01 00:00:00 EST 2020

Lipophilic statins limit cancer cell growth and survival, via involvement of Akt signaling.

PloS one

Beckwitt CH,Shiraha K,Wells A

Mon Nov 26 00:00:00 EST 2018

PLCε1 regulates SDF-1α-induced lymphocyte adhesion and migration to sites of inflammation.

Proceedings of the National Academy of Sciences of the United States of America

Strazza M,Azoulay-Alfaguter I,Peled M,Smrcka AV,Skolnik EY,Srivastava S,Mor A

MA1012 was used in western blot to discover new regulatory proteins involved in Rap1-dependent T-cell adhesion and migration.

Tue Mar 07 00:00:00 EST 2017

Isoprenylcysteine carboxylmethyltransferase is critical for malignant transformation and tumor maintenance by all RAS isoforms.

Oncogene

Lau HY,Tang J,Casey PJ,Wang M

Published figure using Pan Ras monoclonal antibody (Product # MA1-012) in Western Blot

Thu Jul 06 00:00:00 EDT 2017

Pallidin protein in neurodevelopment and its relation to the pathogenesis of schizophrenia.

Molecular medicine reports

Shi Q,Li C,Li K,Liu Q

Published figure using Pan Ras monoclonal antibody (Product # MA1-012) in Western Blot

Wed Feb 01 00:00:00 EST 2017

Calcium-dependent binding of Myc to calmodulin.

Oncotarget

Raffeiner P,Schraffl A,Schwarz T,Röck R,Ledolter K,Hartl M,Konrat R,Stefan E,Bister K

MA1012 was used in western blot to propose that increasing calmodulin levels enhance Myc transcriptional and oncogenic activities

Tue Jan 10 00:00:00 EST 2017

Aurora kinase A interacts with H-Ras and potentiates Ras-MAPK signaling.

Oncotarget

Umstead M,Xiong J,Qi Q,Du Y,Fu H

Published figure using Pan Ras monoclonal antibody (Product # MA1-012) in Immunoprecipitation

Tue Apr 25 00:00:00 EDT 2017

Bipartite Role of Heat Shock Protein 90 (Hsp90) Keeps CRAF Kinase Poised for Activation.

The Journal of biological chemistry

Mitra S,Ghosh B,Gayen N,Roy J,Mandal AK

MA1012 was used in western blot to demonstrate the bipartite role of Hsp90 in chaperoning CRAF kinase

Fri Nov 18 00:00:00 EST 2016

Inhibition of RAS activation due to a homozygous ezrin variant in patients with profound intellectual disability.

Human mutation

Riecken LB,Tawamie H,Dornblut C,Buchert R,Ismayel A,Schulz A,Schumacher J,Sticht H,Pohl KJ,Cui Y,Reis A,Morrison H,Abou Jamra R

MA1-012 was used in western blot to report that ezrin is required for Ras activation.

Sun Feb 01 00:00:00 EST 2015

An improved isoprenylcysteine carboxylmethyltransferase inhibitor induces cancer cell death and attenuates tumor growth in vivo.

Cancer biology & therapy

Lau HY,Ramanujulu PM,Guo D,Yang T,Wirawan M,Casey PJ,Go ML,Wang M

MA1-012 was used in western blot to show that 8.12 is a pharmacological inhibitor of Icmt

Mon Sep 01 00:00:00 EDT 2014

Production and characterization of anti-RAS p21 monoclonal antibodies.

Hybridoma

Hamer PJ,Trimpe KL,Pullano T,Ng S,LaVecchio JA,Petit DA,DeLellis R,Wolfe H,Carney WP

MA1-012 was used in [flow cytometry] to characterize the antibody's reactivity to normal p21, activated p21, and p21 from each of the Harvey, Kirsten and N-ras families.

Sat Dec 01 00:00:00 EST 1990

生物信息学

蛋白别名: c-H-ras; c-Ha-ras p21 protein; c-Ha-ras transgene; c-has/bas p21 protein; c-K-ras; c-Ki-ras; c-Kirsten-ras protein; c-ras-Ki-2 activated oncogene; cellular c-Ki-ras2; cellular c-Ki-ras2 proto-oncogene; GTP- and GDP-binding peptide B; GTPase HRas; GTPase Hras1; GTPase KRas; GTPase NRas; H-ras 1 protein; H-Ras-1; Ha-Ras; Ha-Ras1 proto-oncoprotein; Harvey rat sarcoma viral oncogene homolog; Harvey rat sarcoma viral oncoprotein; Harvey rat sarcoma virus oncogene 1; K RAS; K-Ras 2; K-ras p21 protein; Ki-Ras; Kirsten rat sarcoma oncogene 2, expressed; Kirsten rat sarcoma viral oncogene homolog; N-ras protein part 4; Neuroblastoma RAS; neuroblastoma RAS viral (v-ras) oncogene homolog; oncogene KRAS2; p19 H-RasIDX protein; p21 protein; p21ras; PR310 c-K-ras oncogene; Ras family small GTP binding protein H-Ras; RASK; RASN; transformation gene: oncogene HAMSV; Transforming protein N-Ras; Transforming protein p21; v-Ha-ras Harvey rat sarcoma viral oncogene homolog; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; v-ras neuroblastoma RAS viral oncogene homolog

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基因别名: AI929937; ALPS4; AV095280; C-BAS/HAS; C-H-RAS; c-Ha-ras; C-HA-RAS1; C-K-RAS; c-rasHa; CFC2; CMNS; CTLO; H-ras; H-RASIDX; Ha-ras; HAMSV; Harvey-ras; HRAS; Hras-1; HRAS1; K-ras; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS; Kras-2; KRAS1; KRAS2; N-ras; NCMS; NRAS; NRAS1; NS; NS3; NS6; p21B; p21ras; RALD; ras; RASH1; RASK2

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功能

GTPase activity protein binding GTP binding protein C-terminus binding nucleotide binding GMP binding GDP binding LRR domain binding protein complex binding small GTPase

参与通路

Pan Ras Monoclonal Antibody (Ras10)

Pan Ras Antibody (MA1-012) in ICC/IF

产品信息

MA1-012

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

免疫沉淀 (IP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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