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HSP60 Antibody (MA3-013) in ICC/IF
Immunofluorescent analysis of Heat Shock Protein 60 (Hsp60) in U251 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 60 (Hsp60) monoclonal antibody (Product # MA3-013) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Heat Shock Protein 60 (Hsp60) staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images w... View More
产品信息
MA3-013
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
已发表种属
Human,
Rabbit
宿主/亚型
Mouse
/ IgM
分类
Monoclonal
类型
Antibody
克隆号
2E1/53
抗原
Human placental HSP60.
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
purified
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_325461
产品详细信息
MA3-013 detects HSP60 from human, monkey, mouse, and rat samples. This antibody displays no other protein reactivity.
MA3-013 has been successfully used in Western blot, immunoprecipitation, immunohistochemistry, immunofluorescence, and ELISA procedures. By Western blot, this antibody detects a single 58 kDa protein representing recombinant human HSP60.
The MA3-013 antigen is human placental HSP60. Epitope mapping studies using human HSP60 deletion mutants suggest that this antibody binds between amino acids 211-288.
Reconstitute with PBS.
靶标信息
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 60 kDa chaperonin; 60 kDa heat shock protein; 60 kDa heat shock protein, mitochondrial; Chaperonin 60; CPN60; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1; heat shock 60kDa protein 1 (chaperonin); Heat shock protein; Heat shock protein 60; heat shock protein 60 (liver); heat shock protein 65; Heat shock protein family D member 1; heat shock protein, 60 kDa; HSP; HSP-60; HSP-65; Hsp60s1; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1
基因别名: 60kDa; CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HSPD1; Hspd1-30p; HuCHA60; SPG13
UniProt ID: (Human) P10809, (Mouse) P63038, (Rat) P63039
Entrez Gene ID: (Human) 3329, (Mouse) 15510, (Rat) 63868
lipopolysaccharide binding
p53 binding
DNA replication origin binding
single-stranded DNA binding
double-stranded RNA binding
protein binding
ATP binding
ATPase activity
ubiquitin protein ligase binding
poly(A) RNA binding
unfolded protein binding
chaperone binding
nucleotide binding
protease binding
protein complex binding
insulin binding
protein heterodimerization activity
misfolded protein binding
detection of misfolded protein
B cell cytokine production
MyD88-dependent toll-like receptor signaling pathway
positive regulation of T cell mediated immune response to tumor cell
protein folding
activation of cysteine-type endopeptidase activity involved in apoptotic process
response to unfolded protein
positive regulation of interferon-alpha production
positive regulation of interferon-gamma production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-6 production
protein refolding
B cell proliferation
T cell activation
B cell activation
positive regulation of macrophage activation
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
isotype switching to IgG isotypes
positive regulation of inflammatory response
protein stabilization
positive regulation of T cell activation
chaperone mediated protein folding requiring cofactor
'de novo' protein folding
response to cold
viral process
chaperone-mediated protein complex assembly
protein maturation
response to hypoxia
response to heat
response to organic substance
response to organic cyclic compound
response to lipopolysaccharide
response to ATP
response to cocaine
response to drug
response to hydrogen peroxide
response to estrogen
