This calculator generates a pipetting table for setting up qPCR reactions with Thermo Scientific DyNAmo qPCR Kits.

  1. Fill in the volume of template DNA you want to use, the number of qPCR reactions you intend to run, and the individual reaction volume.
  2. Define if you would like to add some extra volume for pipetting. You can choose between an absolute volume, a number of extra reactions, or a percentage of the final volume.
  3. Fill in your primer stock concentrations and the desired final primer concentrations.
  4. If your reaction includes ROX passive reference dye or a probe, define the respective concentrations.

The application will automatically calculate the pipetting volumes required for a premix and the volume of the premix needed for the final reaction.

Volume of DNA template/reaction µL  
 Extra volume
 Extra reaction
 % of volume
Number of reactions
Reaction volumeµL
Total volumeµL Extra volume%


 
Concentration
Volume (µL)
 
stock
 
final
 
per reaction
 
for premix
2X master mix X X  
Forward primer µM µM  
Reverse primer µM µM  
Other components
ROX X X  
Probe µM µM  

H2O  
Premix  
Template µL

仅供科研使用，不可用于诊断目的。