EVOS Objectives

All EVOS objectives offer outstanding optical performance from visible light to near-infrared light. Choose from over 40 high-performance objective lenses ranging from 1.25–100X using the selection tool below. Before you begin, consider the following when choosing objectives.

There are three types of microscopy objectives, each corresponding to their level of correction for chromatic aberrations:

• Fluorite objectives: exceptional for fluorescence and demanding transmitted-light applications. They enable excellent resolution, resulting in bright fluorescence signal and high-contrast imaging. Fluorite objectives help reduce optical aberrations. Color and focus have a higher level of correction than with other objectives.
• Achromat objectives: excellent for general applications. Color and focus have standard correction compared to apochromat and fluorite objectives.
• Apochromat objectives: excellent for the most demanding applications, especially capturing color images in white light. They enable the highest level of resolution, fluorescence brightness, contrast, and chromatic correction compared to achromat and fluorite objectives.

With each type of microscopy objective, there are two classes:

• Long working distance (LWD) objectives: optimized for vessels with a nominal wall thickness of 0.9–1.5 mm, such as slides, cell culture dishes and flasks, and microtiter plates.
• Coverslip-corrected (CC) objectives: optimized for imaging through 1.5 coverslips (approximately 0.17 mm thick); have a higher magnification-to-numerical aperture (NA) ratio and enable higher resolution than LWD objectives.

Other general guidelines include:

• If your samples are in plastic vessels and you image samples using fluorescence, use fluorite LWD objectives.
• If your samples are in plastic vessels and you image samples using transmitted light, use either achromat or fluorite LWD objectives.
• If your samples are under glass coverslips and you want the best image quality, use apochromatic CC or fluorite CC objectives.