Invitrogen

CD197 (CCR7) Monoclonal Antibody (3D12), FITC, eBioscience™

CD197 (CCR7) Antibody in Flow Cytometry (Flow)

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CD197 (CCR7) Antibody (11-1979-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD45RA APC (Product # 17-0458-42) and Rat IgG2a K Isotype Control FITC (Product # 11-4321-42) (left) or Anti-Human CD197 (CCR7) FITC (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

11-1979-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.5 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

3D12

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10717950

产品详细信息

Description: The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding.

Applications Reported: This 3D12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 3D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

It is recommended that the staining incubation time be increased to at least 45 minutes at 2-8°C for optimal staining.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CCR7 is a member of the G protein coupled receptor family, which is a subfamily of chemokines. CCR7 was identified to be induced by the Epstein Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. CCR7 has been reported to be expressed in blood, bone marrow, lymph node, and intestine. CCR7 is particularly expressed in lymphoid tissues and in activated B and T lymphocytes and has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. ESTs have been isolated from blood, embryo, lymph node, and thymus libraries. Receptors for the C - C chemokine family include CCR 1, CCR 2A, CCR 3, CCR 4, CCR 5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Enhancement of CAR-T cell activity against cholangiocarcinoma by simultaneous knockdown of six inhibitory membrane proteins.

Cancer communications (London, England)

Qiao Y,Chen J,Wang X,Yan S,Tan J,Xia B,Chen Y,Lin K,Zou F,Liu B,He X,Zhang Y,Zhang X,Zhang H,Wu X,Lu L

Published figure using CD197 (CCR7) monoclonal antibody (Product # 11-1979-42) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Neuroimmune Consequences of eIF4E Phosphorylation on Chemotherapy-Induced Peripheral Neuropathy.

Frontiers in immunology

Agalave NM,Mody PH,Szabo-Pardi TA,Jeong HS,Burton MD

Published figure using CD197 (CCR7) monoclonal antibody (Product # 11-1979-42) in Flow Cytometry

Mon Sep 20 00:00:00 EDT 2021

Alteration of the Immune Microenvironment in HBsAg and HBeAg Dual-Positive Pregnant Women Presenting a High HBV Viral Load.

Journal of inflammation research

Gao F,Wang H,Li X,Guo F,Yuan Y,Wang X,Zhang Y,Bai G

Published figure using CD197 (CCR7) monoclonal antibody (Product # 11-1979-42) in Flow Cytometry

Thu Apr 28 00:00:00 EDT 2022

Application of the chemokine-chemokine receptor axis increases the tumor-targeted migration ability of cytokine-induced killer cells in patients with colorectal cancer.

Oncology letters

Zou Y,Liang J,Li D,Fang J,Wang L,Wang J,Zhang J,Guo Q,Yan X,Tang H

Published figure using CD197 (CCR7) monoclonal antibody (Product # 11-1979-42) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Development of CAR-T cell therapy for B-ALL using a point-of-care approach.

Oncoimmunology

de Macedo Abdo L,Barros LRC,Saldanha Viegas M,Vieira Codeço Marques L,de Sousa Ferreira P,Chicaybam L,Bonamino MH

Published figure using CD197 (CCR7) monoclonal antibody (Product # 11-1979-42) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Undernutrition is associated with perturbations in T cell-, B cell-, monocyte- and dendritic cell- subsets in latent Mycobacterium tuberculosis infection.

PloS one

Rajamanickam A,Munisankar S,Dolla CK,Babu S

11-1979 was used in Flow cytometry/Cell sorting to demonstrate that coexistent undernutrition in LTBI is characterized by the occurrence of a significant modulation in the frequency of innate and adaptive immune cell subsets.

Mon Apr 06 00:00:00 EDT 2020

Biochanin a Enhances the Defense Against Salmonella enterica Infection Through AMPK/ULK1/mTOR-Mediated Autophagy and Extracellular Traps and Reversing SPI-1-Dependent Macrophage (MΦ) M2 Polarization.

Frontiers in cellular and infection microbiology

Zhao X,Tang X,Guo N,An Y,Chen X,Shi C,Wang C,Li Y,Li S,Xu H,Liu M,Wang Y,Yu L

11-1979 was used in Flow cytometry/Cell sorting to demonstrate that biochanin A can enhance both intra- and extracellular bactericidal activity of host cells.

Fri Aug 30 00:00:00 EDT 2019

Altered levels of memory T cell subsets and common γc cytokines in Strongyloides stercoralis infection and partial reversal following anthelmintic treatment.

PLoS neglected tropical diseases

Rajamanickam A,Munisankar S,Bhootra Y,Dolla CK,Thiruvengadam K,Nutman TB,Babu S

11-1979 was used in Flow cytometry/Cell sorting to show a significant positive correlation between the levels of IL-7 and the numbers of central and effector memory CD4+ T cells.

Tue May 01 00:00:00 EDT 2018

Oral Supplementation with Baker's Yeast Beta Glucan Is Associated with Altered Monocytes, T Cells and Cytokines following a Bout of Strenuous Exercise.

Frontiers in physiology

McFarlin BK,Venable AS,Carpenter KC,Henning AL,Ogenstad S

Published figure using CD197 (CCR7) monoclonal antibody (Product # 11-1979-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Melanoma cells homing to the brain: an in vitro model.

BioMed research international

Rizzo A,Vasco C,Girgenti V,Fugnanesi V,Calatozzolo C,Canazza A,Salmaggi A,Rivoltini L,Morbin M,Ciusani E

11-1979 was used in Flow cytometry/Cell sorting to reveal that matrix metalloproteinase proteins could be directly involved in blood brain barrier permeability and that brain invasion by melanoma cells could be related to their expression.

Tue Nov 10 00:00:00 EST 2015

CD55 costimulation induces differentiation of a discrete T regulatory type 1 cell population with a stable phenotype.

Journal of immunology (Baltimore, Md. : 1950)

Sutavani RV,Bradley RG,Ramage JM,Jackson AM,Durrant LG,Spendlove I

11-1979 was used in Flow cytometry/Cell sorting to demonstrate that CD55 is a potent costimulator and activator of human naive CD4(+) cells.

Sun Dec 15 00:00:00 EST 2013

Dendritic Cells (DC) Facilitate Detachment of Squamous Carcinoma Cells (SCC), While SCC Promote an Immature CD16(+) DC Phenotype and Control DC Migration.

Cancer microenvironment : official journal of the International Cancer Microenvironment Society

Ramanathapuram LV,Hopkin D,Kurago ZB

11-1979 was used in Flow cytometry/Cell sorting to study the relationship between dendritic cells and squamous carcinoma cells in the tumour microenvironment.

Mon Apr 01 00:00:00 EDT 2013

Circulating T regulatory cells migration and phenotype in glioblastoma patients: an in vitro study.

Journal of neuro-oncology

Vasco C,Canazza A,Rizzo A,Mossa A,Corsini E,Silvani A,Fariselli L,Salmaggi A,Ciusani E

11-1979-42 was used in Sample Preparation to provide evidence that CCL2 is implicated in Treg migration, other factors are needed as well to provide such effect.

Sun Dec 01 00:00:00 EST 2013

Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions.

PLoS pathogens

Qiu Y,Chen J,Liao H,Zhang Y,Wang H,Li S,Luo Y,Fang D,Li G,Zhou B,Shen L,Chen CY,Huang D,Cai J,Cao K,Jiang L,Zeng G,Chen ZW

11-1979 was used in Flow cytometry/Cell sorting to uncover a previously unknown mechanism for T-cell immune responses regulated by Tim-3, which may have implications for potential immune intervention in TB.

Fri Apr 19 00:00:00 EDT 2013

The PD-1/PD-L1 axis modulates the natural killer cell versus multiple myeloma effect: a therapeutic target for CT-011, a novel monoclonal anti-PD-1 antibody.

Blood

Benson DM,Bakan CE,Mishra A,Hofmeister CC,Efebera Y,Becknell B,Baiocchi RA,Zhang J,Yu J,Smith MK,Greenfield CN,Porcu P,Devine SM,Rotem-Yehudar R,Lozanski G,Byrd JC,Caligiuri MA

11-1979 was used in Flow cytometry/Cell sorting to examine the NK cell response against MM, mediated by the PD-1/PD-L1 signalling axis and CT-011.

Thu Sep 30 00:00:00 EDT 2010

Role of TLX1 in T-cell acute lymphoblastic leukaemia pathogenesis.

British journal of haematology

Riz I,Hawley TS,Johnston H,Hawley RG

11-1979 was used in Flow cytometry/Cell sorting to investigate the contribution of TLX1 to the malignant T-cell acute lymphoblastic leukemia phenotype.

Wed Apr 01 00:00:00 EDT 2009

生物信息学

蛋白别名: BLR2; Bukitt's lymphoma receptor 2; C-C chemokine receptor type 7; C-C CKR-7; CC chemokine receptor 7; CD197; CDw197; chemokine (C-C motif) receptor 7; EBI1; EBV-induced G protein-coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus-induced G-protein coupled receptor 1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor

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基因别名: BLR2; CC-CKR-7; CCR-7; CCR7; CD197; CDw197; CMKBR7; EBI1; EVI1

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UniProt ID: (Human) P32248

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Entrez Gene ID: (Human) 1236

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功能

G-protein coupled receptor activity C-C chemokine receptor activity chemokine (C-C motif) ligand 19 binding chemokine (C-C motif) ligand 21 binding C-C motif chemokine 19 receptor activity C-C motif chemokine 21 receptor activity

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