Invitrogen

CD197 (CCR7) Monoclonal Antibody (3D12), APC-eFluor™ 780, eBioscience™

CD197 (CCR7) Antibody in Flow Cytometry (Flow)

图: 1 / 10

CD197 (CCR7) Antibody (47-1979-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD45RA FITC (Product # 11-0458-42) and Rat IgG2a K Isotype Control APC-eFluor® 780 (Product # 47-4321-82) (left) or Anti-Human CD197 (CCR7) APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

产品信息

47-1979-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (1 µg)/test

查看16篇已发表文章 16篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Rhesus monkey

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

3D12

偶联物

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

激发/发射光谱

756/785 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1518794

产品详细信息

Description: The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding.

Applications Reported: This 3D12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 3D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

It is recommended that the staining incubation time be increased to at least 45 minutes at 2-8°C.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CCR7 is a member of the G protein coupled receptor family, which is a subfamily of chemokines. CCR7 was identified to be induced by the Epstein Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. CCR7 has been reported to be expressed in blood, bone marrow, lymph node, and intestine. CCR7 is particularly expressed in lymphoid tissues and in activated B and T lymphocytes and has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. ESTs have been isolated from blood, embryo, lymph node, and thymus libraries. Receptors for the C - C chemokine family include CCR 1, CCR 2A, CCR 3, CCR 4, CCR 5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Enhancement of CAR-T cell activity against cholangiocarcinoma by simultaneous knockdown of six inhibitory membrane proteins.

Cancer communications (London, England)

Qiao Y,Chen J,Wang X,Yan S,Tan J,Xia B,Chen Y,Lin K,Zou F,Liu B,He X,Zhang Y,Zhang X,Zhang H,Wu X,Lu L

Published figure using CD197 (CCR7) monoclonal antibody (Product # 47-1979-42) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Neuroimmune Consequences of eIF4E Phosphorylation on Chemotherapy-Induced Peripheral Neuropathy.

Frontiers in immunology

Agalave NM,Mody PH,Szabo-Pardi TA,Jeong HS,Burton MD

Published figure using CD197 (CCR7) monoclonal antibody (Product # 47-1979-42) in Flow Cytometry

Mon Sep 20 00:00:00 EDT 2021

Alteration of the Immune Microenvironment in HBsAg and HBeAg Dual-Positive Pregnant Women Presenting a High HBV Viral Load.

Journal of inflammation research

Gao F,Wang H,Li X,Guo F,Yuan Y,Wang X,Zhang Y,Bai G

Published figure using CD197 (CCR7) monoclonal antibody (Product # 47-1979-42) in Flow Cytometry

Thu Apr 28 00:00:00 EDT 2022

Targets of T Cell Responses to SARS-CoV-2 Coronavirus in Humans with COVID-19 Disease and Unexposed Individuals.

Cell

Grifoni A,Weiskopf D,Ramirez SI,Mateus J,Dan JM,Moderbacher CR,Rawlings SA,Sutherland A,Premkumar L,Jadi RS,Marrama D,de Silva AM,Frazier A,Carlin AF,Greenbaum JA,Peters B,Krammer F,Smith DM,Crotty S,Sette A

47-1979 was used in Flow cytometry/Cell sorting to analyze the immune cell responses to SARS-CoV-2 of recovered patients to identify regions of the virus that are targeted and reveal cross-reactivity with other common circulating coronaviruses.

Thu Jun 25 00:00:00 EDT 2020

Application of the chemokine-chemokine receptor axis increases the tumor-targeted migration ability of cytokine-induced killer cells in patients with colorectal cancer.

Oncology letters

Zou Y,Liang J,Li D,Fang J,Wang L,Wang J,Zhang J,Guo Q,Yan X,Tang H

Published figure using CD197 (CCR7) monoclonal antibody (Product # 47-1979-42) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Development of CAR-T cell therapy for B-ALL using a point-of-care approach.

Oncoimmunology

de Macedo Abdo L,Barros LRC,Saldanha Viegas M,Vieira Codeço Marques L,de Sousa Ferreira P,Chicaybam L,Bonamino MH

Published figure using CD197 (CCR7) monoclonal antibody (Product # 47-1979-42) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Intracellular delivery of mRNA to human primary T cells with microfluidic vortex shedding.

Scientific reports

Jarrell JA,Twite AA,Lau KHWJ,Kashani MN,Lievano AA,Acevedo J,Priest C,Nieva J,Gottlieb D,Pawell RS

47-1979 was used in Flow cytometry/Cell sorting to show microfluidic vortex shedding mediates intracellular delivery of mRNA into primary human T lymphocytes.

Fri Mar 01 00:00:00 EST 2019

Biochanin a Enhances the Defense Against Salmonella enterica Infection Through AMPK/ULK1/mTOR-Mediated Autophagy and Extracellular Traps and Reversing SPI-1-Dependent Macrophage (MΦ) M2 Polarization.

Frontiers in cellular and infection microbiology

Zhao X,Tang X,Guo N,An Y,Chen X,Shi C,Wang C,Li Y,Li S,Xu H,Liu M,Wang Y,Yu L

Published figure using CD197 (CCR7) monoclonal antibody (Product # 47-1979-42) in Flow Cytometry

Fri Aug 30 00:00:00 EDT 2019

Oral Supplementation with Baker's Yeast Beta Glucan Is Associated with Altered Monocytes, T Cells and Cytokines following a Bout of Strenuous Exercise.

Frontiers in physiology

McFarlin BK,Venable AS,Carpenter KC,Henning AL,Ogenstad S

Published figure using CD197 (CCR7) monoclonal antibody (Product # 47-1979-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Temporal Dynamics of CD8+ T Cell Effector Responses during Primary HIV Infection.

PLoS pathogens

Demers KR,Makedonas G,Buggert M,Eller MA,Ratcliffe SJ,Goonetilleke N,Li CK,Eller LA,Rono K,Maganga L,Nitayaphan S,Kibuuka H,Routy JP,Slifka MK,Haynes BF,McMichael AJ,Bernard NF,Robb ML,Betts MR

47-1979 was used in Flow cytometry/Cell sorting to assess the CD8+ T cell functional evolution from primary to chronic HIV infection, demonstrating that progressive transcriptional dysregulation leads to the reduced CD8+ T cell perforin expression and loss of cytolytic potential characteristic of chronic HIV infection.

Mon Aug 01 00:00:00 EDT 2016

Vaccine Induction of Lymph Node-Resident Simian Immunodeficiency Virus Env-Specific T Follicular Helper Cells in Rhesus Macaques.

Journal of immunology (Baltimore, Md. : 1950)

Vargas-Inchaustegui DA,Demers A,Shaw JM,Kang G,Ball D,Tuero I,Musich T,Mohanram V,Demberg T,Karpova TS,Li Q,Robert-Guroff M

47-1979 was used in Flow cytometry/Cell sorting to quantify SIV Env-specific IL-21-producing T follicular helper cells in response to vaccination.

Mon Feb 15 00:00:00 EST 2016

PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer.

Cancer immunology research

Severson JJ,Serracino HS,Mateescu V,Raeburn CD,McIntyre RC,Sams SB,Haugen BR,French JD

47-1979 was used in Flow cytometry/Cell sorting to determine that the release of PD-1 and Tim-3, and the inhibition of Tregs, may encourage tumour elimination in metastatic differentiated thyroid cancer.

Mon Jun 01 00:00:00 EDT 2015

Select host restriction factors are associated with HIV persistence during antiretroviral therapy.

AIDS (London, England)

Abdel-Mohsen M,Wang C,Strain MC,Lada SM,Deng X,Cockerham LR,Pilcher CD,Hecht FM,Liegler T,Richman DD,Deeks SG,Pillai SK

47-1979 was used in Flow cytometry/Cell sorting to determine the impact of cell-intrinsic immunity on the HIV latent reservoir.

Fri Feb 20 00:00:00 EST 2015

Comprehensive analysis of dengue virus-specific responses supports an HLA-linked protective role for CD8+ T cells.

Proceedings of the National Academy of Sciences of the United States of America

Weiskopf D,Angelo MA,de Azeredo EL,Sidney J,Greenbaum JA,Fernando AN,Broadwater A,Kolla RV,De Silva AD,de Silva AM,Mattia KA,Doranz BJ,Grey HM,Shresta S,Peters B,Sette A

47-1979 was used in Flow cytometry/Cell sorting to analyse dengue virus-specific responses, supporting an HLA-linked protective role for CD8+ T cells.

Tue May 28 00:00:00 EDT 2013

Differential localization of T-bet and Eomes in CD8 T cell memory populations.

Journal of immunology (Baltimore, Md. : 1950)

McLane LM,Banerjee PP,Cosma GL,Makedonas G,Wherry EJ,Orange JS,Betts MR

47-1979 was used in Flow cytometry/Cell sorting to further understand the role of the T-box transcription factors T-bet and Eomes in human CD8 T cell differentiation.

Mon Apr 01 00:00:00 EDT 2013

Role of TLX1 in T-cell acute lymphoblastic leukaemia pathogenesis.

British journal of haematology

Riz I,Hawley TS,Johnston H,Hawley RG

Published figure using CD197 (CCR7) monoclonal antibody (Product # 47-1979-42) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2009

生物信息学

蛋白别名: BLR2; Bukitt's lymphoma receptor 2; C-C chemokine receptor type 7; C-C CKR-7; CC chemokine receptor 7; CD197; CDw197; chemokine (C-C motif) receptor 7; EBI1; EBV-induced G protein-coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus-induced G-protein coupled receptor 1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor

查看更多收起

基因别名: BLR2; CC-CKR-7; CCR-7; CCR7; CD197; CDw197; CMKBR7; EBI1; EVI1

查看更多收起

UniProt ID: (Human) P32248

查看更多收起

Entrez Gene ID: (Human) 1236

查看更多收起

功能

G-protein coupled receptor activity C-C chemokine receptor activity chemokine (C-C motif) ligand 19 binding chemokine (C-C motif) ligand 21 binding C-C motif chemokine 19 receptor activity C-C motif chemokine 21 receptor activity

参与通路