Invitrogen

CD185 (CXCR5) Monoclonal Antibody (MU5UBEE), PE, eBioscience™

CD185 (CXCR5) Antibody in Flow Cytometry (Flow)

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CD185 (CXCR5) Antibody (12-9185-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG2b K Isotype Control PE (Product # 12-4732-81) (left) or Anti-Human CD185 (CXCR5) PE (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

12-9185-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

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产品规格

种属反应

Human, Non-human primate, Rhesus monkey

已发表种属

Human, Non-human primate, Rhesus monkey

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

MU5UBEE

抗原

N-terminal extracellular domain

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偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11219877

产品详细信息

Description: The MU5UBEE monoclonal antibody reacts with human and non-human primate CD185. CD185, which is also known as C-X-C chemokine receptor 5 (CXCR5) and Burkitt lymphoma receptor 1 (BLR1), is a seven transmembrane G protein-coupled receptor originally identified in Burkitt's lymphoma. In peripheral blood, CD185 is expressed on B cells, CD4+ T cells (but not Th1 or Th2 cells), as well as on a subpopulation of memory (CD45RO+) T cells. Circulating CD185+ T cells are in a resting state and migrate to the lymph nodes due to expression of CCR7 and CD62L. In tonsil, CD185 is expressed on nearly all CD4+ cells together with CD45RO and activation markers such as CD69 and ICOS. Tonsillar CD185+ cells have been shown to induce antibody production when co-cultured with B cells, thus supporting their role in providing help to B cells. Furthermore, this chemokine receptor plays a critical role in lymphocyte trafficking, in particular T cell migration into the B cell follicles of germinal centers in response to CXCL13, making CD185 an established marker of follicular helper T cells.

The MU5UBEE monoclonal antibody also crossreacts with Rhesus macaque.

Applications Reported: This MU5UBEE antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MU5UBEE antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CXCR5 (C-X-Chemokine Receptor Type 5, CD185) also known as Burkitt lymphoma receptor 1 (BLR1) belongs to the CXC chemokine receptor family. CXCR5 is a G protein-coupled seven transmembrane receptor for chemokine BLC. CXCR5 gene is specifically expressed in Burkitt's lymphoma and lymphatic tissues, such as follicles in lymph nodes, as well as in spleen. Studies of CXCR5 suggest that it plays an essential role in B cell migration. CXCR5 expression has been reported in human blood and salivary gland and in animal lymphatic organs, blood, bone marrow, brain, liver, lymph node, skin, spleen, stomach, and tonsil. ESTs (expressed sequence tags) have been isolated from human brain, germ cell, lung, lymph, and pancreas. G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members currently predicted to exist. All GPCRs share a structural motif consisting of seven membrane-spanning helices, and exist in both active and inactive forms. An array of activating ligands participate in the conformation of GPCRs which leads to signaling via G-proteins and downstream effectors. Research studies have also shown the vast series of reactions which participate in the negative regulation of GPCRs. This "turn-off" activity in GPCRs have tremendous implications for the physiological action of the cell, and continues to drive pharmacological research for new drug candidates. Two blockbuster drugs which have been developed as GPCR-targeted pharmaceuticals are Zyprexa (Eli Lilly) and Claritin (Schering-Plough) which are used in mental health and allergy applications, respectively.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

The magnitude and timing of recalled immunity after breakthrough infection is shaped by SARS-CoV-2 variants.

Immunity

Koutsakos M,Lee WS,Reynaldi A,Tan HX,Gare G,Kinsella P,Liew KC,Taiaroa G,Williamson DA,Kent HE,Stadler E,Cromer D,Khoury DS,Wheatley AK,Juno JA,Davenport MP,Kent SJ

12-9185-42 was used in Flow cytometry/Cell sorting to define the early kinetics of spike-specific humoral and cellular immunity after vaccination of seropositive individuals and after Delta or Omicron breakthrough infection in vaccinated individuals.

Tue Jul 12 00:00:00 EDT 2022

Baricitinib treatment resolves lower-airway macrophage inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques.

Cell

Hoang TN,Pino M,Boddapati AK,Viox EG,Starke CE,Upadhyay AA,Gumber S,Nekorchuk M,Busman-Sahay K,Strongin Z,Harper JL,Tharp GK,Pellegrini KL,Kirejczyk S,Zandi K,Tao S,Horton TR,Beagle EN,Mahar EA,Lee MYH,Cohen J,Jean SM,Wood JS,Connor-Stroud F,Stammen RL,Delmas OM,Wang S,Cooney KA,Sayegh MN,Wang L,Filev PD,Weiskopf D,Silvestri G,Waggoner J,Piantadosi A,Kasturi SP,Al-Shakhshir H,Ribeiro SP,Sekaly RP,Levit RD,Estes JD,Vanderford TH,Schinazi RF,Bosinger SE,Paiardini M

12-9185 was used in Flow cytometry/Cell sorting to investigate the immunologic and virologic efficacy of baricitinib in a rhesus macaque model of SARS-CoV-2 infection.

Thu Jan 21 00:00:00 EST 2021

Protection against SARS-CoV-2 Beta Variant in mRNA-1273 Boosted Nonhuman Primates.

bioRxiv : the preprint server for biology

Corbett KS,Gagne M,Wagner DA,Connell SO,Narpala SR,Flebbe DR,Andrew SF,Davis RL,Flynn B,Johnston TS,Stringham C,Lai L,Valentin D,Van Ry A,Flinchbaugh Z,Werner AP,Moliva JI,Sriparna M,O'Dell S,Schmidt SD,Tucker C,Choi A,Koch M,Bock KW,Minai M,Nagata BM,Alvarado GS,Henry AR,Laboune F,Schramm CA,Zhang Y,Wang L,Choe M,Boyoglu-Barnum S,Shi W,Lamb E,Nurmukhambetova ST,Provost SJ,Donaldson MM,Marquez J,Todd JM,Cook A,Dodson A,Pekosz A,Boritz E,Ploquin A,Doria-Rose N,Pessaint L,Andersen H,Foulds KE,Misasi J,Wu K,Carfi A,Nason MC,Mascola J,Moore IN,Edwards DK,Lewis MG,Suthar MS,Roederer M,McDermott A,Douek DC,Sullivan NJ,Graham BS,Seder RA

12-9185-42 was used in Flow Cytometry to evaluate the immune responses in nonhuman primates that received 100 µg of mRNA-1273 vaccine at 0 and 4 weeks and were boosted at week 29 with mRNA-1273 (homologous) or mRNA-1273.β (heterologous), which encompasses the spike sequence of the B.1.351 (beta or β) variant.

Thu Aug 12 00:00:00 EDT 2021

A modified vaccinia Ankara vector-based vaccine protects macaques from SARS-CoV-2 infection, immune pathology, and dysfunction in the lungs.

Immunity

Routhu NK,Cheedarla N,Gangadhara S,Bollimpelli VS,Boddapati AK,Shiferaw A,Rahman SA,Sahoo A,Edara VV,Lai L,Floyd K,Wang S,Fischinger S,Atyeo C,Shin SA,Gumber S,Kirejczyk S,Cohen J,Jean SM,Wood JS,Connor-Stroud F,Stammen RL,Upadhyay AA,Pellegrini K,Montefiori D,Shi PY,Menachery VD,Alter G,Vanderford TH,Bosinger SE,Suthar MS,Amara RR

12-9185-42 was used in Flow cytometry/Cell sorting to demonstrate that MVA/S vaccination induces neutralizing antibodies and CD8+ T cells in the blood and lungs and is a potential vaccine candidate for SARS-CoV-2.

Tue Mar 09 00:00:00 EST 2021

CTLA-4 and PD-1 dual blockade induces SIV reactivation without control of rebound after antiretroviral therapy interruption.

Nature medicine

Harper J,Gordon S,Chan CN,Wang H,Lindemuth E,Galardi C,Falcinelli SD,Raines SLM,Read JL,Nguyen K,McGary CS,Nekorchuk M,Busman-Sahay K,Schawalder J,King C,Pino M,Micci L,Cervasi B,Jean S,Sanderson A,Johns B,Koblansky AA,Amrine-Madsen H,Lifson J,Margolis DM,Silvestri G,Bar KJ,Favre D,Estes JD,Paiardini M

12-9185 was used in Flow cytometry/Cell sorting to demonstrate that PD-1, CTLA-4 and dual CTLA-4/PD-1 immune checkpoint blockade using monoclonal antibodies is well tolerated, with evidence of bioactivity in blood and lymph nodes.

Wed Apr 01 00:00:00 EDT 2020

Humoral and circulating follicular helper T cell responses in recovered patients with COVID-19.

Nature medicine

Juno JA,Tan HX,Lee WS,Reynaldi A,Kelly HG,Wragg K,Esterbauer R,Kent HE,Batten CJ,Mordant FL,Gherardin NA,Pymm P,Dietrich MH,Scott NE,Tham WH,Godfrey DI,Subbarao K,Davenport MP,Kent SJ,Wheatley AK

12-9185 was used in Flow cytometry/Cell sorting to characterize humoral and circulating follicular helper T cell (cTFH) immunity against spike in recovered patients with coronavirus disease 2019 (COVID-19).

Tue Sep 01 00:00:00 EDT 2020

E4BP4-mediated inhibition of T follicular helper cell differentiation is compromised in autoimmune diseases.

The Journal of clinical investigation

Wang Z,Zhao M,Yin J,Liu L,Hu L,Huang Y,Liu A,Ouyang J,Min X,Rao S,Zhou W,Wu H,Yoshimura A,Lu Q

12-9185 was used in Flow cytometry/Cell sorting to study the mediatory role of the adenovirus E4BP4 within Tfh cell differentiation and study the immunological changes seen within patients with systemic lupus erythematosus.

Wed Jul 01 00:00:00 EDT 2020

Baricitinib treatment resolves lower airway inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques.

bioRxiv : the preprint server for biology

Hoang TN,Pino M,Boddapati AK,Viox EG,Starke CE,Upadhyay AA,Gumber S,Busman-Sahay K,Strongin Z,Harper JL,Tharp GK,Pellegrini KL,Kirejczyk S,Zandi K,Tao S,Horton TR,Beagle EN,Mahar EA,Lee MY,Cohen J,Jean SM,Wood JS,Connor-Stroud F,Stammen RL,Delmas OM,Wang S,Cooney KA,Sayegh MN,Wang L,Weiskopf D,Filev PD,Waggoner J,Piantadosi A,Kasturi SP,Al-Shakhshir H,Ribeiro SP,Sekaly RP,Levit RD,Estes JD,Vanderford TH,Schinazi RF,Bosinger SE,Paiardini M

12-9185 was used in Flow cytometry/Cell sorting to investigate the immunologic and virologic efficacy of baricitinib in a rhesus macaque model of SARS-CoV-2 infection.

Wed Sep 16 00:00:00 EDT 2020

Baricitinib treatment resolves lower airway inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques.

bioRxiv : the preprint server for biology

12-9185 was used in Flow cytometry/Cell sorting to investigate the immunologic and virologic efficacy of baricitinib in a rhesus macaque model of SARS-CoV-2 infection.

Wed Sep 16 00:00:00 EDT 2020

Distribution of circulating follicular helper T cells and expression of interleukin-21 and chemokine C-X-C ligand 13 in gastric cancer.

Oncology letters

Meng X,Yu X,Dong Q,Xu X,Li J,Xu Q,Ma J,Zhou C

12-9185-42 was used in Flow cytometry/Cell sorting to investigate the distribution of cTfh cells, and the expression of IL-21 and CXCL13 in patients with gastric cancer was evaluated in order to ascertain the significance and potential mechanisms of these effectors in gastric cancer.

Sat Sep 01 00:00:00 EDT 2018

Dynamics of SIV-specific CXCR5+ CD8 T cells during chronic SIV infection.

Proceedings of the National Academy of Sciences of the United States of America

Mylvaganam GH,Rios D,Abdelaal HM,Iyer S,Tharp G,Mavigner M,Hicks S,Chahroudi A,Ahmed R,Bosinger SE,Williams IR,Skinner PJ,Velu V,Amara RR

12918542 was used in flow cytometry to study CXCR5+ CD8 T cells in SIV-infected animals

Tue Feb 21 00:00:00 EST 2017

Follicular Regulatory CD8 T Cells Impair the Germinal Center Response in SIV and Ex Vivo HIV Infection.

PLoS pathogens

Miles B,Miller SM,Folkvord JM,Levy DN,Rakasz EG,Skinner PJ,Connick E

12918542 was used in flow cytometry to identify and characterize CXCR5hiCD44hiCD8 T cells in HIV-infected individuals

Sat Oct 01 00:00:00 EDT 2016

PD-L1hi B cells are critical regulators of humoral immunity.

Nature communications

Khan AR,Hams E,Floudas A,Sparwasser T,Weaver CT,Fallon PG

12-9185 was used in Flow cytometry/Cell sorting to demonstrate that elevated PD-L1 expression on B cells is an important regulator of T follicular helper-cell activity, downstream antibody responses and autoimmune disease.

Thu Jan 22 00:00:00 EST 2015

Diminished viral control during simian immunodeficiency virus infection is associated with aberrant PD-1hi CD4 T cell enrichment in the lymphoid follicles of the rectal mucosa.

Journal of immunology (Baltimore, Md. : 1950)

Mylvaganam GH,Velu V,Hong JJ,Sadagopal S,Kwa S,Basu R,Lawson B,Villinger F,Amara RR

12-9185 was used in Flow cytometry/Cell sorting to investigate the role of PD-1 in CD4 T cells, showing that decreased viral control in SIV is associated with aberrant PD-1hi CD4 T cell enrichment in the lymphoid follicles of the rectal mucosa.

Sat Nov 01 00:00:00 EDT 2014

Compartmentalization of simian immunodeficiency virus replication within secondary lymphoid tissues of rhesus macaques is linked to disease stage and inversely related to localization of virus-specific CTL.

Journal of immunology (Baltimore, Md. : 1950)

Connick E,Folkvord JM,Lind KT,Rakasz EG,Miles B,Wilson NA,Santiago ML,Schmitt K,Stephens EB,Kim HO,Wagstaff R,Li S,Abdelaal HM,Kemp N,Watkins DI,MaWhinney S,Skinner PJ

12-9185 was used in Flow cytometry/Cell sorting to quantify specific immune cells present in a number of tissues during different stages of HIV infection.

Mon Dec 01 00:00:00 EST 2014

Early lymphoid responses and germinal center formation correlate with lower viral load set points and better prognosis of simian immunodeficiency virus infection.

Journal of immunology (Baltimore, Md. : 1950)

Hong JJ,Amancha PK,Rogers KA,Courtney CL,Havenar-Daughton C,Crotty S,Ansari AA,Villinger F

12-9185 was used in Flow cytometry/Cell sorting to investigate the dynamics of germinal center formation in lymphoid tissues following acute SIV infection.

Tue Jul 15 00:00:00 EDT 2014

生物信息学

蛋白别名: Blr-1; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5); Burkitt lymphoma receptor 1, GTP-binding protein; C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 5; CC motif chemokine; CCmotif chemokine; CD185; CD185 antigen; chemokine (C-X-C motif) receptor 5; CXC; CXC motif chemokine; CXC-R5; CXCR-5; GPR9; HUMSTR; MDR-15; Monocyte-derived receptor 15

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基因别名: BLR1; CD185; CXCR5; EGK_06966; MDR15

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UniProt ID: (Human) P32302

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Entrez Gene ID: (Rhesus monkey) 701792, (Human) 643

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功能

G-protein coupled receptor activity protein binding C-X-C chemokine receptor activity

参与通路

movement of cell or subcellular component chemotaxis immune response G-protein coupled receptor signaling pathway positive regulation of cytokinesis B cell activation lymph node development chemokine-mediated signaling pathway