Invitrogen

CD185 (CXCR5) Monoclonal Antibody (MU5UBEE), PE-eFluor™ 610, eBioscience™

CD185 (CXCR5) Antibody in Flow Cytometry (Flow)

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CD185 (CXCR5) Antibody (61-9185-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG2b K Isotype Control PE-eFluor® 610 (Product # 61-4732-82) (left) or Anti-Human CD185 (CXCR5) PE-eFluor® 610 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

61-9185-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

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产品规格

种属反应

Human, Non-human primate, Rhesus monkey

已发表种属

Human, Non-human primate, Rhesus monkey

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

MU5UBEE

抗原

N-terminal extracellular domain

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偶联物

PE-eFluor™ 610 PE-eFluor™ 610 PE-eFluor™ 610

激发/发射光谱

565/606 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2574677

产品详细信息

Description: The MU5UBEE monoclonal antibody reacts with human and non-human primate CD185. CD185, which is also known as C-X-C chemokine receptor 5 (CXCR5) and Burkitt lymphoma receptor 1 (BLR1), is a seven transmembrane G protein-coupled receptor originally identified in Burkitt's lymphoma. In peripheral blood, CD185 is expressed on B cells, CD4+ T cells (but not Th1 or Th2 cells), as well as on a subpopulation of memory (CD45RO+) T cells. Circulating CD185+ T cells are in a resting state and migrate to the lymph nodes due to expression of CCR7 and CD62L. In tonsil, CD185 is expressed on nearly all CD4+ cells together with CD45RO and activation markers such as CD69 and ICOS. Tonsillar CD185+ cells have been shown to induce antibody production when co-cultured with B cells, thus supporting their role in providing help to B cells. Furthermore, this chemokine receptor plays a critical role in lymphocyte trafficking, in particular T cell migration into the B cell follicles of germinal centers in response to CXCL13, making CD185 an established marker of follicular helper T cells.

The MU5UBEE monoclonal antibody also crossreacts with Rhesus macaque.

Applications Reported: This MU5UBEE antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MU5UBEE antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CXCR5 (C-X-Chemokine Receptor Type 5, CD185) also known as Burkitt lymphoma receptor 1 (BLR1) belongs to the CXC chemokine receptor family. CXCR5 is a G protein-coupled seven transmembrane receptor for chemokine BLC. CXCR5 gene is specifically expressed in Burkitt's lymphoma and lymphatic tissues, such as follicles in lymph nodes, as well as in spleen. Studies of CXCR5 suggest that it plays an essential role in B cell migration. CXCR5 expression has been reported in human blood and salivary gland and in animal lymphatic organs, blood, bone marrow, brain, liver, lymph node, skin, spleen, stomach, and tonsil. ESTs (expressed sequence tags) have been isolated from human brain, germ cell, lung, lymph, and pancreas. G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members currently predicted to exist. All GPCRs share a structural motif consisting of seven membrane-spanning helices, and exist in both active and inactive forms. An array of activating ligands participate in the conformation of GPCRs which leads to signaling via G-proteins and downstream effectors. Research studies have also shown the vast series of reactions which participate in the negative regulation of GPCRs. This "turn-off" activity in GPCRs have tremendous implications for the physiological action of the cell, and continues to drive pharmacological research for new drug candidates. Two blockbuster drugs which have been developed as GPCR-targeted pharmaceuticals are Zyprexa (Eli Lilly) and Claritin (Schering-Plough) which are used in mental health and allergy applications, respectively.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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61-9185-42 was used in Flow cytometry/Cell sorting to show that, by increasing zinc availability, SAMT-247 increases natural killer cytotoxicity and monocyte efferocytosis, and decreases T-cell activation to augment vaccine-induced protection.

Mon May 01 00:00:00 EDT 2023

Phenotypic and functional characterization of pharmacologically expanded Vγ9Vδ2 T cells in pigtail macaques.

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Barber-Axthelm IM,Wragg KM,Esterbauer R,Amarasena TH,Barber-Axthelm VRB,Wheatley AK,Gibbon AM,Kent SJ,Juno JA

61-9185-42 was used in Flow cytometry/Cell sorting to show the tissue distribution and phenotype of in vivo pharmacologically expanded Vγ9Vδ2 T cells can be altered based on the antigen administration route, with implications for tissue trafficking and the clinical efficacy of Vγ9Vδ2 T cell immunotherapeutics.

Fri Mar 17 00:00:00 EDT 2023

Spike-specific circulating T follicular helper cell and cross-neutralizing antibody responses in COVID-19-convalescent individuals.

Nature microbiology

Zhang J,Wu Q,Liu Z,Wang Q,Wu J,Hu Y,Bai T,Xie T,Huang M,Wu T,Peng D,Huang W,Jin K,Niu L,Guo W,Luo D,Lei D,Wu Z,Li G,Huang R,Lin Y,Xie X,He S,Deng Y,Liu J,Li W,Lu Z,Chen H,Zeng T,Luo Q,Li YP,Wang Y,Liu W,Qu X

61-9185 was used in Flow cytometry/Cell sorting to provide insight into neutralizing antibody responses in COVID-19-convalescent individuals and facilitate the treatment and vaccine development for SARS-CoV-2 infection.

Fri Jan 01 00:00:00 EST 2021

HIV vaccine candidate activation of hypoxia and the inflammasome in CD14⁺ monocytes is associated with a decreased risk of SIV_mac251 acquisition.

Nature medicine

Vaccari M,Fourati S,Gordon SN,Brown DR,Bissa M,Schifanella L,Silva de Castro I,Doster MN,Galli V,Omsland M,Fujikawa D,Gorini G,Liyanage NPM,Trinh HV,McKinnon KM,Foulds KE,Keele BF,Roederer M,Koup RA,Shen X,Tomaras GD,Wong MP,Munoz KJ,Gach JS,Forthal DN,Montefiori DC,Venzon DJ,Felber BK,Rosati M,Pavlakis GN,Rao M,Sekaly RP,Franchini G

61-9185 was used in Flow cytometry/Cell sorting to examine the ability of several vaccine prime modalities together with two ALVAC-SIV + gp120 protein boosts to reduce the risk of SIVmac251 acquisition in Rhesus macaques.

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Perturbed CD8⁺ T cell TIGIT/CD226/PVR axis despite early initiation of antiretroviral treatment in HIV infected individuals.

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61918542 was used in flow cytometry to show T cell immunoglobulin and ITIM domain expression increases over time despite early initiation of antiretroviral treatment

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Regulatory and Helper Follicular T Cells and Antibody Avidity to Simian Immunodeficiency Virus Glycoprotein 120.

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Blackburn MJ,Zhong-Min M,Caccuri F,McKinnon K,Schifanella L,Guan Y,Gorini G,Venzon D,Fenizia C,Binello N,Gordon SN,Miller CJ,Franchini G,Vaccari M

61-9185 was used in Flow cytometry/Cell sorting to demonstrate changes in the TFH/TFR ratio during chronic infection and suggest possible mechanisms for the unchecked expansion of TFH cells in HIV/SIV infection.

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生物信息学

蛋白别名: Blr-1; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5); Burkitt lymphoma receptor 1, GTP-binding protein; C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 5; CC motif chemokine; CCmotif chemokine; CD185; CD185 antigen; chemokine (C-X-C motif) receptor 5; CXC; CXC motif chemokine; CXC-R5; CXCR-5; GPR9; HUMSTR; MDR-15; Monocyte-derived receptor 15

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基因别名: BLR1; CD185; CXCR5; EGK_06966; MDR15

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UniProt ID: (Human) P32302

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Entrez Gene ID: (Rhesus monkey) 701792, (Human) 643

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功能

G-protein coupled receptor activity protein binding C-X-C chemokine receptor activity

参与通路

movement of cell or subcellular component chemotaxis immune response G-protein coupled receptor signaling pathway positive regulation of cytokinesis B cell activation lymph node development chemokine-mediated signaling pathway