Invitrogen

CD11a (LFA-1alpha) Monoclonal Antibody (M17/4), eBioscience™

CD11a (LFA-1alpha) Antibody in Flow Cytometry (Flow)

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CD11a (LFA-1alpha) Antibody (14-0111-82) in Flow

Staining of mouse splenocytes with Anti-Mouse CD11a FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

CD11a (LFA-1alpha) Antibody in Immunohistochemistry (IHC)

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产品信息

14-0111-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

Assay-Dependent

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.5 µg/test

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免疫沉淀 (IP)

Assay-Dependent

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中和实验 (Neu)

Assay-Dependent

功能检测 (Functional)

Assay-Dependent

抑制试剂 (IA)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

M17/4

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_467105

产品详细信息

Description: The M17/4 monoclonal antibody reacts with mouse CD11a, the 180 kDa integrin alpha L, also known as the lymphocyte function associated antigen-1 (LFA-1) alpha chain. LFA-1, formed by non-covalent association of CD11a with CD18 (integrin beta 2), serves as an important adhesion molecule involved in lymphocyte and granulocyte function. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are ligands for LFA-1. CD11a is expressed by all leukocytes.

Applications Reported: The M17/4 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. M17/4 has also been reported in blocking of CD11a function. (Please use Functional Grade purified M17/4, Product # 16-0111, in functional assays).

Applications Tested: The M17/4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD11a (LFA-1 alpha, ITGAL) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. CD11a is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of CD11a to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. CD11a is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of CD11a function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti- CD11a antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. Two transcript variants encoding different isoforms of CD11a have been found. Diseases associated with CD11a dysfunction include benign cephalic histiocytosis and leukocyte adhesion deficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Modulating CD40 and integrin signaling in the proinflammatory nexus using a 15-amino-acid peptide, KGYY₁₅.

The Journal of biological chemistry

Vaitaitis GM,Wagner DH

Published figure using CD11a (LFA-1alpha) monoclonal antibody (Product # 14-0111-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2023

Adhesion Molecule Profile and the Effect of Anti-VLA-4 mAb Treatment in Experimental Autoimmune Encephalomyelitis, a Mouse Model of Multiple Sclerosis.

International journal of molecular sciences

Pyka-Fościak G,Lis GJ,Litwin JA

Published figure using CD11a (LFA-1alpha) monoclonal antibody (Product # 14-0111-82) in Immunohistochemistry

Fri Apr 22 00:00:00 EDT 2022

Importance of the Immunodominant CD8⁺ T Cell Epitope of Plasmodium berghei Circumsporozoite Protein in Parasite- and Vaccine-Induced Protection.

Infection and immunity

Gibbins MP,Müller K,Glover M,Liu J,Putrianti ED,Bauza K,Reyes-Sandoval A,Matuschewski K,Silvie O,Hafalla JCR

14-0111-82 was used in Flow Cytometry to characterise the importance of the immunodominant circumsporozoite protein-derived epitope SYIPSAEKI of Plasmodium berghei in both sporozoite- and vaccine-induced protection in murine infection models.

Fri Sep 18 00:00:00 EDT 2020

Early IL-6 signalling promotes IL-27 dependent maturation of regulatory T cells in the lungs and resolution of viral immunopathology.

PLoS pathogens

Pyle CJ,Uwadiae FI,Swieboda DP,Harker JA

14-0111-82 was used in Flow Cytometry to show that during respiratory virus infection the prototypic inflammatory cytokine IL-6 is a critical anti-inflammatory regulator of viral induced immunopathology in the respiratory tract through its induction of IL-27.

Fri Sep 01 00:00:00 EDT 2017

Endothelial cell-derived CD95 ligand serves as a chemokine in induction of neutrophil slow rolling and adhesion.

eLife

Gao L,Gülcüler GS,Golbach L,Block H,Zarbock A,Martin-Villalba A

14-0111 was used in Flow cytometry/Cell sorting to identify the mechanisms underlying the chemoattractant effect of endothelial cell-derived CD95L in induction of neutrophil recruitment.

Thu Oct 20 00:00:00 EDT 2016

Endothelial cell-derived CD95 ligand serves as a chemokine in induction of neutrophil slow rolling and adhesion.

eLife

Gao L,Gülcüler GS,Golbach L,Block H,Zarbock A,Martin-Villalba A

14-0111 was used in Flow cytometry/Cell sorting to identify the mechanisms underlying the chemoattractant effect of endothelial cell-derived CD95L in induction of neutrophil recruitment.

Thu Oct 20 00:00:00 EDT 2016

Toxoplasma gondii-infected natural killer cells display a hypermotility phenotype in vivo.

Immunology and cell biology

Ueno N,Lodoen MB,Hickey GL,Robey EA,Coombes JL

14-0111 was used in Flow cytometry/Cell sorting to investigate whether T.gondii spreads by inducing a hypermotility phenotype in parasitised immune cells in vivo.

Wed Feb 17 00:00:00 EST 2016

Ly6C(high) monocytes control cerebral toxoplasmosis.

Journal of immunology (Baltimore, Md. : 1950)

Biswas A,Bruder D,Wolf SA,Jeron A,Mack M,Heimesaat MM,Dunay IR

14-0111 was used in Flow cytometry/Cell sorting to indicate the critical importance of recruited Ly6C monocytes upon cerebral toxoplasmosis.

Wed Apr 01 00:00:00 EDT 2015

Regulation of asymmetric division and CD8+ T lymphocyte fate specification by protein kinase Cζ and protein kinase Cλ/ι.

Journal of immunology (Baltimore, Md. : 1950)

Metz PJ,Arsenio J,Kakaradov B,Kim SH,Remedios KA,Oakley K,Akimoto K,Ohno S,Yeo GW,Chang JT

14-0111 was used in Immunocytochemistry-immunoflourescence to show that PKCζ and PKCλ/i regulate asymmetric localization of effector fate-associated factors during the first CD8+ T cell division in vivo.

Sun Mar 01 00:00:00 EST 2015

Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression.

Shock (Augusta, Ga.)

Shindo Y,Unsinger J,Burnham CA,Green JM,Hotchkiss RS

Published figure using CD11a (LFA-1alpha) monoclonal antibody (Product # 14-0111-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Cellular size as a means of tracking mTOR activity and cell fate of CD4+ T cells upon antigen recognition.

PloS one

Pollizzi KN,Waickman AT,Patel CH,Sun IH,Powell JD

14-0111 was used in Flow cytometry/Cell sorting to characterise the metabolism and immunologic functions of distinct clonal populations of activated T cells.

Fri Mar 25 00:00:00 EDT 2016

Microsphere priming facilitates induction of potent therapeutic T-cell immune responses against autochthonous liver cancers.

European journal of immunology

Brinkhoff B,Ostroumov D,Heemcke J,Woller N,Gürlevik E,Manns MP,Longerich T,Zender L,Harty JT,Kubicka S,Kühnel F,Wirth TC

14-0111-82 was used in Flow Cytometry to develop a prime-boost vaccination protocol based on the administration of antigen conjugated to poly-lactic-co-glycolic acid microspheres followed by booster vaccination with Listeria monocytogenes vectors.

Tue Apr 01 00:00:00 EDT 2014

Polymicrobial sepsis alters antigen-dependent and -independent memory CD8 T cell functions.

Journal of immunology (Baltimore, Md. : 1950)

Duong S,Condotta SA,Rai D,Martin MD,Griffith TS,Badovinac VP

14-0111 was used in Flow cytometry/Cell sorting to study the role of sepsis in shaping the quantity and functionality of infection- or vaccine-induced memory CD8 T cells.

Tue Apr 15 00:00:00 EDT 2014

IRF4 in dendritic cells inhibits IL-12 production and controls Th1 immune responses against Leishmania major.

Journal of immunology (Baltimore, Md. : 1950)

Akbari M,Honma K,Kimura D,Miyakoda M,Kimura K,Matsuyama T,Yui K

14-0111 was used in Flow cytometry/Cell sorting to investigate the roles of the transcription factor IRF4 in dendritic cells and macrophages in vivo.

Sat Mar 01 00:00:00 EST 2014

Identification of targets of CD8⁺ T cell responses to malaria liver stages by genome-wide epitope profiling.

PLoS pathogens

Hafalla JC,Bauza K,Friesen J,Gonzalez-Aseguinolaza G,Hill AV,Matuschewski K

14-0111 was used in Flow cytometry/Cell sorting to investigate antigenic epitopes in a murine malaria immunisation model.

Wed May 01 00:00:00 EDT 2013

ADAM17 activation in circulating neutrophils following bacterial challenge impairs their recruitment.

Journal of leukocyte biology

Long C,Hosseinkhani MR,Wang Y,Sriramarao P,Walcheck B

14-0111 was used in Flow cytometry/Cell sorting to study how impaired L-selectin shedding is a key mechanism underlying early neutrophil recruitment in conditional ADAM17 knockout mice during bacterial infection.

Sat Sep 01 00:00:00 EDT 2012

Contact-dependent T cell activation and T cell stopping require talin1.

Journal of immunology (Baltimore, Md. : 1950)

Wernimont SA,Wiemer AJ,Bennin DA,Monkley SJ,Ludwig T,Critchley DR,Huttenlocher A

14-0111 was used in Inhibition experiments to investigate the role of Talin1 in contact-dependent T cell activation and T cell stopping.

Thu Dec 15 00:00:00 EST 2011

Role for E-cadherin as an inhibitory receptor on epidermal gammadelta T cells.

Journal of immunology (Baltimore, Md. : 1950)

Uchida Y,Kawai K,Ibusuki A,Kanekura T

14-0111 was used in Flow cytometry/Cell sorting to analyse functional differences between E-cadherin and α(E)β(7) integrin expressed on murine dendritic epidermal T cells.

Wed Jun 15 00:00:00 EDT 2011

PIPKI gamma 90 negatively regulates LFA-1-mediated adhesion and activation in antigen-induced CD4+ T cells.

Journal of immunology (Baltimore, Md. : 1950)

Wernimont SA,Legate KR,Simonson WT,Fassler R,Huttenlocher A

14-0111 was used in Immunofluorescence to demonstrate the activity of PIPKI gamma 90 as a novel negative regulator of antigen-induced CD4+ T cell adhesion and activation.

Fri Oct 15 00:00:00 EDT 2010

Cognate CD4 help is essential for the reactivation and expansion of CD8 memory T cells directed against the hematopoietic cell-specific dominant minor histocompatibility antigen, H60.

Blood

Ryu SJ,Jung KM,Yoo HS,Kim TW,Kim S,Chang J,Choi EY

14-0111 was used in Flow cytometry/Cell sorting to provide insight into the control of minor H antigen-specific CD8 T-cell responses, to maximize the graft-versus-leukemia response.

Thu Apr 30 00:00:00 EDT 2009

Plasmacytoid dendritic cells regulate autoreactive B cell activation via soluble factors and in a cell-to-cell contact manner.

Journal of immunology (Baltimore, Md. : 1950)

Ding C,Cai Y,Marroquin J,Ildstad ST,Yan J

14-0111 was used in Flow cytometry/Cell sorting to suggest the dynamic interplay between pDCs and B cells is required for autoreactive B cell activation upon stimulation.

Tue Dec 01 00:00:00 EST 2009

生物信息学

蛋白别名: alpha L integrin; antigen CD11A p180; CD11 antigen-like family member A; CD11a; Integrin alpha-L; integrin alphaL; Leukocyte adhesion glycoprotein LFA-1 alpha chain; Leukocyte function-associated molecule 1 alpha chain; LFA-1A; Ly-15; Lymphocyte antigen 15; lymphocyte function associated antigen 1, alpha polypeptide

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基因别名: (p180); Cd11a; Itgal; LFA-1; LFA-1A; Ly-15; Ly-21

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UniProt ID: (Mouse) P24063

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Entrez Gene ID: (Mouse) 16408

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功能

ICAM-3 receptor activity protein complex binding metal ion binding protein heterodimerization activity cell adhesion molecule binding integrin

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