Invitrogen

CD11a (LFA-1alpha) Monoclonal Antibody (M17/4), Functional Grade, eBioscience™

CD11a (LFA-1alpha) Antibody in Flow Cytometry (Flow)

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CD11a (LFA-1alpha) Antibody (16-0111-82) in Flow

Staining of C57Bl/6 splenocytes with 0.25 µg of Rat IgG2a K Isotype Control Functional Grade Purified (Product # 16-4321-82) (open histogram) or 0.25 µg of Anti-Mouse CD11a Functional Grade Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85).Cells in the lymphocyte gate were used for analysis.

CD11a (LFA-1alpha) Antibody in Immunohistochemistry (IHC)

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产品信息

16-0111-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.5 µg/test

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中和实验 (Neu)

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功能检测 (Functional)

Assay-Dependent

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对照试剂 (Ctrl)

Assay-Dependent

抑制试剂 (IA)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

M17/4

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_468881

产品详细信息

Description: The M17/4 monoclonal antibody reacts with mouse CD11a, the 180 kDa integrin alpha L, also known as the lymphocyte function associated antigen-1 (LFA-1) alpha chain. LFA-1, formed by non-covalent association of CD11a with CD18 (integrin beta 2), serves as an important adhesion molecule involved in lymphocyte and granulocyte function. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are ligands for LFA-1. CD11a is expressed by all leukocytes.

Applications Reported: The M17/4 antibody has been reported for use in flow cytometric analysis. M17/4 has also been reported in blocking of CD11a function.

Applications Tested: The M17/4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

CD11a (LFA-1 alpha, ITGAL) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. CD11a is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of CD11a to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. CD11a is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of CD11a function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti- CD11a antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. Two transcript variants encoding different isoforms of CD11a have been found. Diseases associated with CD11a dysfunction include benign cephalic histiocytosis and leukocyte adhesion deficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Modulating CD40 and integrin signaling in the proinflammatory nexus using a 15-amino-acid peptide, KGYY₁₅.

The Journal of biological chemistry

Vaitaitis GM,Wagner DH

Published figure using CD11a (LFA-1alpha) monoclonal antibody (Product # 16-0111-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2023

Adhesion Molecule Profile and the Effect of Anti-VLA-4 mAb Treatment in Experimental Autoimmune Encephalomyelitis, a Mouse Model of Multiple Sclerosis.

International journal of molecular sciences

Pyka-Fościak G,Lis GJ,Litwin JA

Published figure using CD11a (LFA-1alpha) monoclonal antibody (Product # 16-0111-82) in Immunohistochemistry

Fri Apr 22 00:00:00 EDT 2022

The opposing forces of shear flow and sphingosine-1-phosphate control marginal zone B cell shuttling.

Nature communications

Tedford K,Steiner M,Koshutin S,Richter K,Tech L,Eggers Y,Jansing I,Schilling K,Hauser AE,Korthals M,Fischer KD

16-0111-82 was used in Functional assay to establish shear flow as a directional cue for marginal zone B cell migration to the follicle, and define sphingosine-1-phosphate receptor 3 and VCAM-1 as counteracting forces that inhibit this migration.

Fri Dec 22 00:00:00 EST 2017

Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration.

Nature communications

Brinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JS

16-0111 was used in Blocking experiments to studying Treg cell engagement with the lymphotoxin beta receptor in transendothelial migration.

Tue Jun 21 00:00:00 EDT 2016

Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration.

Nature communications

Brinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JS

16-0111 was used in Blocking experiments to studying Treg cell engagement with the lymphotoxin beta receptor in transendothelial migration.

Tue Jun 21 00:00:00 EDT 2016

A Novel Model of Asymptomatic Plasmodium Parasitemia That Recapitulates Elements of the Human Immune Response to Chronic Infection.

PloS one

Fontana MF,Baccarella A,Craft JF,Boyle MJ,McIntyre TI,Wood MD,Thorn KS,Anidi C,Bayat A,Chung MR,Hamburger R,Kim CY,Pearman E,Pham J,Tang JJ,Boon L,Kamya MR,Dorsey G,Feeney ME,Kim CC

16-0111 was used in Flow cytometry/Cell sorting to investigate aspects of the human immune response to asymptomatic persistent Plasmodium infection using a novel animal model.

Wed Aug 02 00:00:00 EDT 2017

CD8+ T cell exhaustion, suppressed gamma interferon production, and delayed memory response induced by chronic Brucella melitensis infection.

Infection and immunity

Durward-Diioia M,Harms J,Khan M,Hall C,Smith JA,Splitter GA

16-0111 was used in Flow cytometry/Cell sorting to assess the populations of immune cells which respond to chronic infection of Brucella melitensis.

Tue Dec 01 00:00:00 EST 2015

Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression.

Shock (Augusta, Ga.)

Shindo Y,Unsinger J,Burnham CA,Green JM,Hotchkiss RS

Published figure using CD11a (LFA-1alpha) monoclonal antibody (Product # 16-0111-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Neutrophil crawling in capillaries; a novel immune response to Staphylococcus aureus.

PLoS pathogens

Harding MG,Zhang K,Conly J,Kubes P

16-0111 was used in Blocking experiments to elucidate the importance of neutrophils in protection against MRSA-induced soft tissue infections.

Wed Oct 01 00:00:00 EDT 2014

Treatment of experimental stroke with IL-10-producing B-cells reduces infarct size and peripheral and CNS inflammation in wild-type B-cell-sufficient mice.

Metabolic brain disease

Bodhankar S,Chen Y,Vandenbark AA,Murphy SJ,Offner H

16-0111 was used in Flow cytometry/Cell sorting to investigate the beneficial immunoregulatory effects of IL-10-producing B-cell stroke treatment in mice.

Sat Mar 01 00:00:00 EST 2014

Blockade of LFA-1 augments in vitro differentiation of antigen-induced Foxp3⁺ Treg cells.

Journal of immunological methods

Verhagen J,Wraith DC

16-0111 was used in Functional assays to identify factors that allow the generation of antigen-specific iTreg cells at higher purities.

Mon Dec 01 00:00:00 EST 2014

Homotypic NK cell-to-cell communication controls cytokine responsiveness of innate immune NK cells.

Scientific reports

Kim TJ,Kim M,Kim HM,Lim SA,Kim EO,Kim K,Song KH,Kim J,Kumar V,Yee C,Doh J,Lee KM

16-0111 was used in Flow cytometry/Cell sorting to demonstrate that innate immune natural killer (NK) cells can engage in homotypic NK-to-NK cell interactions.

Fri Dec 05 00:00:00 EST 2014

ICAM-1-dependent homotypic aggregates regulate CD8 T cell effector function and differentiation during T cell activation.

Journal of immunology (Baltimore, Md. : 1950)

Zumwalde NA,Domae E,Mescher MF,Shimizu Y

16-0111 was used in Flow cytometry/Cell sorting to demonstrate that during CD8 T cell activation, the T cell clustering mechanism allows for continued proliferation of T cells, and regulates CD8 T cell effector function and differentiation.

Tue Oct 01 00:00:00 EDT 2013

Marginal zone B cells regulate antigen-specific T cell responses during infection.

Journal of immunology (Baltimore, Md. : 1950)

Bankoti R,Gupta K,Levchenko A,Stäger S

16-0111 was used in Functional assays to investigate the role of B cells in the regulation of antigen-specific CD8+ T cell responses in a murine model of visceral leishmaniasis.

Sun Apr 15 00:00:00 EDT 2012

Multiple CD4+ T cell subsets produce immunomodulatory IL-10 during respiratory syncytial virus infection.

Journal of immunology (Baltimore, Md. : 1950)

Weiss KA,Christiaansen AF,Fulton RB,Meyerholz DK,Varga SM

16-0111 was used in Flow cytometry/Cell sorting to evaluate the role of IL-10 in the modulation of the respiratory syncytial virus-specific immune response.

Thu Sep 15 00:00:00 EDT 2011

Extreme CD8 T cell requirements for anti-malarial liver-stage immunity following immunization with radiation attenuated sporozoites.

PLoS pathogens

Schmidt NW,Butler NS,Badovinac VP,Harty JT

16-0111 was used in Flow cytometry/Cell sorting to identify and track whole-parasite, RAS-vaccine-induced effector and memory CD8 T cell responses.

Thu Jul 15 00:00:00 EDT 2010

Distinct roles of complement receptor 3, Dectin-1, and sialic acids in murine macrophage interaction with Histoplasma yeast.

Journal of leukocyte biology

Lin JS,Huang JH,Hung LY,Wu SY,Wu-Hsieh BA

16-0111 was used in Blocking experiments to delineate the distinct roles of CR3, Dectin-1, and sialic acids in the interaction with Histoplasma.

Thu Jul 01 00:00:00 EDT 2010

Calpain 4 is not necessary for LFA-1-mediated function in CD4+ T cells.

PloS one

Wernimont SA,Simonson WT,Greer PA,Seroogy CM,Huttenlocher A

16-0111 was used in Immunofluorescence to study calpain regulation of LFA-1 function.

Fri May 07 00:00:00 EDT 2010

Neuropilin-1 expression on regulatory T cells enhances their interactions with dendritic cells during antigen recognition.

Immunity

Sarris M,Andersen KG,Randow F,Mayr L,Betz AG

16-0111 was used in Blocking experiments to investigate the role of neutopilin-1 in interactions between Tregs and dendritic cells.

Sat Mar 01 00:00:00 EST 2008

Enumeration and functional evaluation of virus-specific CD4+ and CD8+ T cells in lymphoid and peripheral sites of coxsackievirus B3 infection.

Journal of virology

Kemball CC,Harkins S,Whitton JL

16-0111 was used in Flow cytometry/Cell sorting to evaluate CVB3 epitope-specific CD8(+) and CD4(+) T-cell responses in the spleen, heart and pancreas of mice in vivo.

Thu May 01 00:00:00 EDT 2008

Stable masking by H-2Dd cis ligand limits Ly49A relocalization to the site of NK cell/target cell contact.

Proceedings of the National Academy of Sciences of the United States of America

Back J,Chalifour A,Scarpellino L,Held W

16-0111 was used in Flow cytometry/Cell sorting to investigate whether masked Ly49A remains constitutively associated with cis D(d) during target cell interaction.

Tue Mar 06 00:00:00 EST 2007

生物信息学

蛋白别名: alpha L integrin; antigen CD11A p180; CD11 antigen-like family member A; CD11a; Integrin alpha-L; integrin alphaL; Leukocyte adhesion glycoprotein LFA-1 alpha chain; Leukocyte function-associated molecule 1 alpha chain; LFA-1A; Ly-15; Lymphocyte antigen 15; lymphocyte function associated antigen 1, alpha polypeptide

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基因别名: (p180); Cd11a; Itgal; LFA-1; LFA-1A; Ly-15; Ly-21

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UniProt ID: (Mouse) P24063

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Entrez Gene ID: (Mouse) 16408

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功能

ICAM-3 receptor activity protein complex binding metal ion binding protein heterodimerization activity cell adhesion molecule binding integrin

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