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SIGLEC H Antibody (14-0333-82) in Flow
Cell reports 2020 -
Figure 1. Presence of pDCs in Naive Corneas and Alterations in Their Density upon Sterile Inflammation and Acute HSV-1 Keratitis (A and B) Flow cytometric analysis of pooled corneal single-cell suspensions of naive mice (n = 15-20 corneas) and 3 days post-thermal cautery (n = 4-8 corneas). (A) Flow cytometry density plots illustrating PDCA-1 + CD45R/B220 + cells among CD45 + cells in naive and inflamed corn... View More
产品信息
14-0333-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rat
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
eBio440c
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1107009
产品详细信息
Description: The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse. mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction.
The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG).
It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.
Applications Tested: This eBio440c antibody has been tested by blocking staining of the direct conjugate of eBio440c. This can be used at less than or equal to 2 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
It is recommended that direct conjugates such as Product # 11-0333-82 be used rather than purified eBio440c antibody when performing flow cytometric analysis.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction. The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG). It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: sialic acid binding Ig-like lectin H; Siglec; SIGLEC-like protein
基因别名: 6430529G09Rik; Siglec-H
Entrez Gene ID: (Mouse) 233274
