Invitrogen

SIGLEC H Monoclonal Antibody (eBio440c), PerCP-eFluor™ 710, eBioscience™

SIGLEC H Antibody in Flow Cytometry (Flow)

图: 1 / 3

SIGLEC H Antibody (46-0333-82) in Flow

Staining of SJL splenocytes with Anti-Human/Mouse CD45R (B220) PE (Product # 12-0452-82) and 0.06 µg of Rat IgG2b K Isotype Control PerCP-eFluor® 710 (Product # 46-4031-82) (left) or 0.06 µg of Anti-Mouse Siglec H PerCP-eFluor® 710 (right). Total viable cells were used for analysis.

Product Image Gallery

产品信息

46-0333-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

查看8篇已发表文章 8篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBio440c

偶联物

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

激发/发射光谱

482/708 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1834443

产品详细信息

Description: The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse. mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction.

The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG).

It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.

Applications Reported: This eBio440c antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio440c antibody has been tested by flow cytometric analysis of SJL spleen cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction. The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG). It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

A lncRNA identifies Irf8 enhancer element in negative feedback control of dendritic cell differentiation.

eLife

Xu H,Li Z,Kuo CC,Götz K,Look T,de Toledo MAS,Seré K,Costa IG,Zenke M

Published figure using SIGLEC H monoclonal antibody (Product # 46-0333-82) in Flow Cytometry

Tue Mar 14 00:00:00 EDT 2023

Characterization of Resident Corneal Plasmacytoid Dendritic Cells and Their Pivotal Role in Herpes Simplex Keratitis.

Cell reports

Jamali A,Hu K,Sendra VG,Blanco T,Lopez MJ,Ortiz G,Qazi Y,Zheng L,Turhan A,Harris DL,Hamrah P

Published figure using SIGLEC H monoclonal antibody (Product # 46-0333-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

An Nfil3-Zeb2-Id2 pathway imposes Irf8 enhancer switching during cDC1 development.

Nature immunology

Bagadia P,Huang X,Liu TT,Durai V,Grajales-Reyes GE,Nitschké M,Modrusan Z,Granja JM,Satpathy AT,Briseño CG,Gargaro M,Iwata A,Kim S,Chang HY,Shaw AS,Murphy TL,Murphy KM

46-0333-82 was used in Flow cytometry/Cell sorting to understand the switch from an E-protein-dependent enhancer to the Batf3-dependent Irf8 enhancer in maturation of classical type 1 dendritic cells (cDC1s).

Sun Sep 01 00:00:00 EDT 2019

Characterization of immune cell subtypes in three commonly used mouse strains reveals gender and strain-specific variations.

Laboratory investigation; a journal of technical methods and pathology

Hensel JA,Khattar V,Ashton R,Ponnazhagan S

Published figure using SIGLEC H monoclonal antibody (Product # 46-0333-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

Batf3 maintains autoactivation of Irf8 for commitment of a CD8α(+) conventional DC clonogenic progenitor.

Nature immunology

Grajales-Reyes GE,Iwata A,Albring J,Wu X,Tussiwand R,Kc W,Kretzer NM,Briseño CG,Durai V,Bagadia P,Haldar M,Schönheit J,Rosenbauer F,Murphy TL,Murphy KM

46-0333 was used in Flow cytometry/Cell sorting to investigate the role of the transcription factors Batf3 and IRF8 in the development of CD8α(+) conventional dendritic cells (cDCs)..

Wed Jul 01 00:00:00 EDT 2015

Notch2-dependent classical dendritic cells orchestrate intestinal immunity to attaching-and-effacing bacterial pathogens.

Nature immunology

Satpathy AT,Briseño CG,Lee JS,Ng D,Manieri NA,Kc W,Wu X,Thomas SR,Lee WL,Turkoz M,McDonald KG,Meredith MM,Song C,Guidos CJ,Newberry RD,Ouyang W,Murphy TL,Stappenbeck TS,Gommerman JL,Nussenzweig MC,Colonna M,Kopan R,Murphy KM

46-0333 was used in Flow cytometry/Cell sorting to elucidate the cellular source of essential IL-23 production required for the mucosal response to attaching-and-effacing bacterial infection.

Sun Sep 01 00:00:00 EDT 2013

A type I IFN-Flt3 ligand axis augments plasmacytoid dendritic cell development from common lymphoid progenitors.

The Journal of experimental medicine

Chen YL,Chen TT,Pai LM,Wesoly J,Bluyssen HA,Lee CK

46-0333 was used in Flow cytometry/Cell sorting to define a critical role for the FL/IFN-I/Flt3 axis in plasmacytoid dendritic cell differentiation from common lymphoid progenitors.

Mon Nov 18 00:00:00 EST 2013

Zbtb46 expression distinguishes classical dendritic cells and their committed progenitors from other immune lineages.

The Journal of experimental medicine

Satpathy AT,KC W,Albring JC,Edelson BT,Kretzer NM,Bhattacharya D,Murphy TL,Murphy KM

46-0333 was used in Flow cytometry/Cell sorting to identify Zbtb46 as a transcription factor selectively expressed by classical DCs and their committed progenitors.