Invitrogen

CD69 Monoclonal Antibody (H1.2F3), APC, eBioscience™

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CD69 Antibody (17-0691-82) in Flow

Staining of 1-day ConA-stimulated BALB/c splenocytes with 0.125 µg of Armenian Hamster IgG Isotype Control APC (Product # 17-4888-82) (blue histogram) or 0.125 µg of Anti-Mouse CD69 APC (purple histogram). Total viable cells were used for analysis.

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产品信息

17-0691-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

查看84篇已发表文章 84篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

H1.2F3

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1210795

产品详细信息

Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.

Applications Reported: This H1.2F3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This H1.2F3 antibody has been tested by flow cytometric analysis of stimulated mouse splenic cell cultures. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Fasudil prevents liver fibrosis via activating natural killer cells and suppressing hepatic stellate cells.

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TRPM8 channel augments T-cell activation and proliferation.

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STING Activated Tumor-Intrinsic Type I Interferon Signaling Promotes CXCR3 Dependent Antitumor Immunity in Pancreatic Cancer.

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GPR120 induces regulatory dendritic cells by inhibiting HK2-dependent glycolysis to alleviate fulminant hepatic failure.

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Robust induction of T_RMs by combinatorial nanoshells confers cross-strain sterilizing immunity against lethal influenza viruses.

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17-0691 was used in Flow cytometry/Cell sorting to identify the control of Treg activation and immune tolerance maintenance is due to Noc4L mediated ribosome biogenesis.

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A Pck1-directed glycogen metabolic program regulates formation and maintenance of memory CD8⁺ T cells.

Nature cell biology

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Intranasal delivery of adjuvant-free peptide nanofibers elicits resident CD8⁺ T cell responses.

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MicroRNA-126 deficiency enhanced the activation and function of CD4⁺ T cells by elevating IRS-1 pathway.

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MAIT cells contribute to protection against lethal influenza infection in vivo.

Nature communications

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Homoharringtonine induced immune alteration for an Efficient Anti-tumor Response in Mouse Models of Non-small Cell Lung Adenocarcinoma Expressing Kras Mutation.

Scientific reports

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Development of a screening strategy for new modulators of T cell receptor signaling and T cell activation.

Scientific reports

Chen EW,Brzostek J,Gascoigne NRJ,Rybakin V

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A method for manual and automated multiplex RNAscope in situ hybridization and immunocytochemistry on cytospin samples.

PloS one

Chan S,Filézac de L'Etang A,Rangell L,Caplazi P,Lowe JB,Romeo V

17-0691 was used in Flow cytometry/Cell sorting to develop a method for manual and automated multiplex RNAscope in situ hybridisation and immunocytochemistry on cytospin samples.

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The Cyclopeptide Astin C Specifically Inhibits the Innate Immune CDN Sensor STING.

Cell reports

Li S,Hong Z,Wang Z,Li F,Mei J,Huang L,Lou X,Zhao S,Song L,Chen W,Wang Q,Liu H,Cai Y,Yu H,Xu H,Zeng G,Wang Q,Zhu J,Liu X,Tan N,Wang C

17-0691 was used in Flow cytometry/Cell sorting to characterise a STING-specific small-molecular inhibitor that may be applied for potentially manipulating the STING-mediated clinical diseases.

Tue Dec 18 00:00:00 EST 2018

Adipose tissue B2 cells promote insulin resistance through leukotriene LTB4/LTB4R1 signaling.

The Journal of clinical investigation

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A type 2 cytokine axis for thymus emigration.

The Journal of experimental medicine

White AJ,Baik S,Parnell SM,Holland AM,Brombacher F,Jenkinson WE,Anderson G

17-0691 was used in Flow cytometry/Cell sorting to show the thymic microenvironment expresses the type 2 IL-4R complex and is functionally responsive to its known ligands, IL-4 and IL-13.

Mon Aug 07 00:00:00 EDT 2017

IL-36 Signaling Facilitates Activation of the NLRP3 Inflammasome and IL-23/IL-17 Axis in Renal Inflammation and Fibrosis.

Journal of the American Society of Nephrology : JASN

Chi HH,Hua KF,Lin YC,Chu CL,Hsieh CY,Hsu YJ,Ka SM,Tsai YL,Liu FC,Chen A

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Sat Jul 01 00:00:00 EDT 2017

FHL2 Regulates Natural Killer Cell Development and Activation during Streptococcus pneumoniae Infection.

Frontiers in immunology

Baranek T,Morello E,Valayer A,Aimar RF,Bréa D,Henry C,Besnard AG,Dalloneau E,Guillon A,Dequin PF,Narni-Mancinelli E,Vivier E,Laurent F,Wei Y,Paget C,Si-Tahar M

17-0691 was used in Flow cytometry/Cell sorting to determine the expression and role of FHL2 in natural killer cell biology.

Wed Nov 20 00:00:00 EST 2019

Betulin from Hedyotis hedyotidea ameliorates concanavalin A-induced and T cell-mediated autoimmune hepatitis in mice.

Acta pharmacologica Sinica

Zhou YQ,Weng XF,Dou R,Tan XS,Zhang TT,Fang JB,Wu XW

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

14-3-3 Proteins regulate K_2P 5.1 surface expression on T lymphocytes.

Traffic (Copenhagen, Denmark)

Fernández-Orth J,Ehling P,Ruck T,Pankratz S,Hofmann MS,Landgraf P,Dieterich DC,Smalla KH,Kähne T,Seebohm G,Budde T,Wiendl H,Bittner S,Meuth SG

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Selectivity of Human TLR9 for Double CpG Motifs and Implications for the Recognition of Genomic DNA.

Journal of immunology (Baltimore, Md. : 1950)

Pohar J,Yamamoto C,Fukui R,Cajnko MM,Miyake K,Jerala R,Benčina M

17-0691 was used in Flow cytometry/Cell sorting to study the selectivity of human TLR9 for double CpG motifs.

Wed Mar 01 00:00:00 EST 2017

IRF6 Regulates Alternative Activation by Suppressing PPARγ in Male Murine Macrophages.

Endocrinology

Li C,Ying W,Huang Z,Brehm T,Morin A,Vella AT,Zhou B

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses.

Nature communications

Wang Q,Ning H,Peng H,Wei L,Hou R,Hoft DF,Liu J

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Wed Oct 11 00:00:00 EDT 2017

Serum Gp96 is a chaperone of complement-C3 during graft-versus-host disease.

JCI insight

Seignez A,Joly AL,Chaumonnot K,Hazoumé A,Sanka M,Marcion G,Boudesco C,Hammann A,Seigneuric R,Jégo G,Ducoroy P,Delarue P,Senet P,Castilla-Llorente C,Solary E,Durey MA,Rubio MT,Hermine O,Kohli E,Garrido C

17-0691 was used in Flow cytometry/Cell sorting to identify glycoprotein 96 and complement C3 as partner graft-versus-host disease-induced tissue damage-associated molecular pattern molecules.

Thu Mar 23 00:00:00 EDT 2017

The efficacy of oral and subcutaneous antigen-specific immunotherapy in murine cow's milk- and peanut allergy models.

Clinical and translational allergy

Vonk MM,Wagenaar L,Pieters RHH,Knippels LMJ,Willemsen LEM,Smit JJ,van Esch BCAM,Garssen J

17-0691 was used in Flow cytometry/Cell sorting to compare oral immunotherapy and subcutaneous immunotherapy models for cows milk and peanut allergy.

Fri Apr 08 00:00:00 EDT 2022

MyD88-dependent pro-interleukin-1β induction in dendritic cells exposed to food-grade synthetic amorphous silica.

Particle and fibre toxicology

Winkler HC,Kornprobst J,Wick P,von Moos LM,Trantakis I,Schraner EM,Bathke B,Hochrein H,Suter M,Naegeli H

Fri Jun 23 00:00:00 EDT 2017

Regulatory T cell frequencies and phenotypes following anti-viral vaccination.

PloS one

de Wolf ACMT,van Aalst S,Ludwig IS,Bodinham CL,Lewis DJ,van der Zee R,van Eden W,Broere F

17-0691 was used in Flow cytometry/Cell sorting to determine the impact of anti-viral vaccination on Treg homeostasis.

Fri Sep 22 00:00:00 EDT 2017

MicroRNA-7 Deficiency Ameliorates the Pathologies of Acute Lung Injury through Elevating KLF4.

Frontiers in immunology

Zhao J,Chen C,Guo M,Tao Y,Cui P,Zhou Y,Qin N,Zheng J,Zhang J,Xu L

17-0691 was used in Flow cytometry/Cell sorting to study the role of microRNA-7 in acute lung injury, allowing the development of new therapeutic strategies against inflammatory lung disease.

Wed Mar 16 00:00:00 EDT 2022

Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.

Journal of immunology (Baltimore, Md. : 1950)

Menendez CM,Jinkins JK,Carr DJ

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Mon Aug 15 00:00:00 EDT 2016

Affinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells.

eLife

Allison KA,Sajti E,Collier JG,Gosselin D,Troutman TD,Stone EL,Hedrick SM,Glass CK

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Mon Jul 04 00:00:00 EDT 2016

The HSP90 inhibitor, 17AAG, protects the intestinal stem cell niche and inhibits graft versus host disease development.

Oncogene

Joly AL,Deepti A,Seignez A,Goloudina A,Hebrard S,Schmitt E,Richaud S,Fourmaux E,Hammann A,Collura A,Svrcek M,Jego G,Robinet E,Solary E,Demidov O,Kohli E,Garrido C

17-0691-82 was used in Flow cytometry/Cell sorting to investigate HSP90 inhibitors in graft versus host disease development in intestinal stem cell niches.

Thu Jun 02 00:00:00 EDT 2016

Alternative splicing of MALT1 controls signalling and activation of CD4(+) T cells.

Nature communications

Meininger I,Griesbach RA,Hu D,Gehring T,Seeholzer T,Bertossi A,Kranich J,Oeckinghaus A,Eitelhuber AC,Greczmiel U,Gewies A,Schmidt-Supprian M,Ruland J,Brocker T,Heissmeyer V,Heyd F,Krappmann D

17-0691 was used in Flow cytometry/Cell sorting to study how TCR-induced alternative splicing augments MALT1 scaffolding to enhance downstream signalling and to promote optimal T-cell activation.

Tue Apr 12 00:00:00 EDT 2016

MicroRNA-223 is a crucial mediator of PPARγ-regulated alternative macrophage activation.

The Journal of clinical investigation

Ying W,Tseng A,Chang RC,Morin A,Brehm T,Triff K,Nair V,Zhuang G,Song H,Kanameni S,Wang H,Golding MC,Bazer FW,Chapkin RS,Safe S,Zhou B

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Mon Nov 02 00:00:00 EST 2015

Single-Dose CpG Immunization Protects Against a Heterosubtypic Challenge and Generates Antigen-Specific Memory T Cells.

Frontiers in immunology

Vogel AJ,Brown DM

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Fri Jul 10 00:00:00 EDT 2015

MFG-E8 Is Critical for Embryonic Stem Cell-Mediated T Cell Immunomodulation.

Stem cell reports

Tan Y,AlKhamees B,Jia D,Li L,Couture JF,Figeys D,Jinushi M,Wang L

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Tue Nov 10 00:00:00 EST 2015

Sunitinib depletes myeloid-derived suppressor cells and synergizes with a cancer vaccine to enhance antigen-specific immune responses and tumor eradication.

Oncoimmunology

Draghiciu O,Nijman HW,Hoogeboom BN,Meijerhof T,Daemen T

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2015

Immunoglobulin E plays an immunoregulatory role in lupus.

The Journal of experimental medicine

Dema B,Charles N,Pellefigues C,Ricks TK,Suzuki R,Jiang C,Scheffel J,Hasni S,Hoffman V,Jablonski M,Sacré K,Gobert D,Papo T,Daugas E,Crampton S,Bolland S,Rivera J

17-0691 was used in Flow cytometry/Cell sorting to explore the effect of IgE deficiency on the inflammatory response in FcγRIIB-deficient mice, which hastens and results in a more aggressive lupuslike disease.

Mon Oct 20 00:00:00 EDT 2014

Peptidylarginine deiminase 4 contributes to tumor necrosis factor α-induced inflammatory arthritis.

Arthritis & rheumatology (Hoboken, N.J.)

Shelef MA,Sokolove J,Lahey LJ,Wagner CA,Sackmann EK,Warner TF,Wang Y,Beebe DJ,Robinson WH,Huttenlocher A

17-0691 was used in Flow cytometry/Cell sorting to investigate whether TNFα and PAD4 may synergistically exacerbate autoantibody production and inflammatory arthritis, showing that PAD4 contributes to TNFα-induced inflammatory arthritis.

Sun Jun 01 00:00:00 EDT 2014

Mechanisms of the immunosuppressive effects of mouse adipose tissue-derived mesenchymal stromal cells on mouse alloreactively stimulated spleen cells.

Experimental and therapeutic medicine

Nagaya R,Mizuno-Kamiya M,Takayama E,Kawaki H,Onoe I,Tanabe T,Nagahara K,Kondoh N

17-0691 was used in Flow cytometry/Cell sorting to demonstrate that adipose tissue-derived mesenchymal stromal cells have significant immune modulatory effects on alloreactively stimulated spleen cells.

Wed Jan 01 00:00:00 EST 2014

Tim-3 negatively mediates natural killer cell function in LPS-induced endotoxic shock.

PloS one

Hou H,Liu W,Wu S,Lu Y,Peng J,Zhu Y,Lu Y,Wang F,Sun Z

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2015

Establishment of a murine graft-versus-myeloma model using allogeneic stem cell transplantation.

PloS one

Binsfeld M,Beguin Y,Belle L,Otjacques E,Hannon M,Briquet A,Heusschen R,Drion P,Zilberberg J,Bogen B,Baron F,Caers J

17-0691 was used in Flow cytometry/Cell sorting to investigate the anti-myeloma effects of allo-SCT from B10.D2 mice into MHC-matched myeloma-bearing Balb/cJ mice.

Tue Dec 15 00:00:00 EST 2015

The thymic medulla is required for Foxp3+ regulatory but not conventional CD4+ thymocyte development.

The Journal of experimental medicine

Cowan JE,Parnell SM,Nakamura K,Caamano JH,Lane PJ,Jenkinson EJ,Jenkinson WE,Anderson G

17-0691 was used in Flow cytometry/Cell sorting to demonstrate a differential requirement for the thymic medulla in relation to CD4 conventional and Foxp3(+) thymocyte lineages.

Mon Apr 08 00:00:00 EDT 2013

Alterations in regulatory T cells induced by specific oligosaccharides improve vaccine responsiveness in mice.

PloS one

Schijf MA,Kerperien J,Bastiaans J,Szklany K,Meerding J,Hofman G,Boon L,van Wijk F,Garssen J,Van't Land B

17-0691 was used in Flow cytometry/Cell sorting to identify factors affecting the quality of the immune response to vaccination.

Tue May 27 00:00:00 EDT 2014

Angiotensin II is a new component involved in splenic T lymphocyte responses during Plasmodium berghei ANKA infection.

PloS one

Silva-Filho JL,Souza MC,Ferreira-Dasilva CT,Silva LS,Costa MF,Padua TA,Henriques Md,Morrot A,Savino W,Caruso-Neves C,Pinheiro AA

17-0691 was used in Flow cytometry/Cell sorting to provide evidence for the potential role of Ang II in regulating the splenic immune response.

Tue Nov 26 00:00:00 EST 2013

Microparticles bearing encephalitogenic peptides induce T-cell tolerance and ameliorate experimental autoimmune encephalomyelitis.

Nature biotechnology

Getts DR,Martin AJ,McCarthy DP,Terry RL,Hunter ZN,Yap WT,Getts MT,Pleiss M,Luo X,King NJ,Shea LD,Miller SD

17-0691 was used in Flow cytometry/Cell sorting to highlight the potential for using microparticles to target natural apoptotic clearance pathways to inactivate pathogenic T cells in autoimmunity.

Sat Dec 01 00:00:00 EST 2012

Dynamic in situ cytometry uncovers T cell receptor signaling during immunological synapses and kinapses in vivo.

Immunity

Moreau HD,Lemaître F,Terriac E,Azar G,Piel M,Lennon-Dumenil AM,Bousso P

17-0691 was used in Flow cytometry/Cell sorting to examine how T cells sense TCR ligands of varying affinities in vivo.

Fri Aug 24 00:00:00 EDT 2012

Anti-HIV B Cell lines as candidate vaccine biosensors.

Journal of immunology (Baltimore, Md. : 1950)

Ota T,Doyle-Cooper C,Cooper AB,Huber M,Falkowska E,Doores KJ,Hangartner L,Le K,Sok D,Jardine J,Lifson J,Wu X,Mascola JR,Poignard P,Binley JM,Chakrabarti BK,Schief WR,Wyatt RT,Burton DR,Nemazee D

17-0691 was used in Flow cytometry/Cell sorting to suggest that HIV Env antigen-expressing, infection-competent virions are poorly recognized by high affinity bNAb-expressing cells.

Thu Nov 15 00:00:00 EST 2012

Commitment to the regulatory T cell lineage requires CARMA1 in the thymus but not in the periphery.

PLoS biology

Barnes MJ,Krebs P,Harris N,Eidenschenk C,Gonzalez-Quintial R,Arnold CN,Crozat K,Sovath S,Moresco EM,Theofilopoulos AN,Beutler B,Hoebe K

17-0691 was used in Flow cytometry/Cell sorting to investigate methods to distinguish between Tregs of thymic or peripheral origin, showing that commitment requires CARMA1 in the thymus but not in the periphery.